| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 125,085 | 2 bp→CA | coding (2069‑2070/2664 nt) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,085 | 0 | T | C | 94.1% | 87.8 / ‑6.2 | 34 | I690T (ATC→ACA) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (15/17); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 125,086 | 0 | C | A | 97.0% | 79.3 / ‑4.7 | 34 | I690T (ATC→ACA) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (14/18); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01 | |||||||||||
TCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATC > NC_000913/124948‑125221 || tCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTGACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTa > 1:658443/1‑143 (MQ=255) cacaTTCAGTCGCGTATTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGaa < 2:270603/143‑1 (MQ=255) aTTCAGTCGCTGACTATCCCGAACTGTATCTAATCGGCTGCAATGAACGCTTACGAAGTTGCTGTCACCATGTATTCCCGTCCGCTCCGTAGTTGCGGTTAATATCATTATCACGTTTACTACTACACAACTACGCGTAACGa > 2:439235/1‑143 (MQ=255) gCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTAcc > 1:272359/1‑143 (MQ=255) cGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATCCCCGCa > 2:650483/1‑143 (MQ=255) gACCCGGCTTAAGCGTTCAAATTGGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGa < 2:470273/143‑1 (MQ=255) cccGGCTTACGCTGACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAACGCCGGAAGGTGCCGAgg > 2:678590/1‑143 (MQ=255) cGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGaa < 2:642820/143‑1 (MQ=255) cGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGGATGTACGGAGAAAAACAAGAGAACGCTTACTACTACACGACTACGCTGAACGAAAAGTACCACATGCCAGCCATGCAGGAAGgcgcaacggaa > 2:359798/1‑136 (MQ=255) ttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGACGGTACCCgca > 2:27361/1‑141 (MQ=255) cgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAAAACGTTCACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAg < 1:369797/143‑1 (MQ=255) cgAAGTTGCTGGCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTGCGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAg < 2:138266/143‑1 (MQ=255) gAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTGCACAACTACGCTGAACGAAAACTACCACATGCCGGCGACGCCGGAAGGAGCTGAGGAAGGCATCCGCaaacg > 2:521677/1‑141 (MQ=255) tcatcaTGCATGACGGTCTGGAGCGTATGGACCGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGCCGGAGATCAGCAAGGTATCCGTAAAGGTATCTACaaa < 2:441662/143‑1 (MQ=255) ggTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGTAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGa < 1:391302/143‑1 (MQ=255) ggTCTGGACCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGa < 1:43584/143‑1 (MQ=255) gTCTGGGGCGTAGGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGaa < 2:505308/143‑1 (MQ=255) tCTGGATCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAg < 2:199121/143‑1 (MQ=255) cTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAggg > 1:402396/1‑143 (MQ=255) tGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGt > 1:245073/1‑143 (MQ=255) tGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACGACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGt < 2:92674/143‑1 (MQ=255) tACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGAGCTGAGGACGGTATCCGTAAAGGTATCTAAAAAGTCGACACTATTGAAGGTCGCAACGGTaa > 2:474295/1‑143 (MQ=255) aCGGTTAAAACGAAGAGAACGCGTACTACTACACAACTACGCTGAAGGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTaaa < 2:206500/143‑1 (MQ=255) aCGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTaaa < 1:439235/143‑1 (MQ=255) aCGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTaaa < 2:84955/143‑1 (MQ=255) aCGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACAAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAGAGGTATCTACAAACTCCAAACAATTGAAGGGAACAAAAGTaaa > 2:444996/1‑143 (MQ=255) tGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGCGGAAGCTATCCGCAAAGGTATCTACAAACTCGACACTATTCAAGGTAGCAAAGGtcagctcc > 2:193475/1‑136 (MQ=255) tGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGCAAAGGTATCTACAAACACGAAACAATTGAAGGTAGCAAAGGTAAACTTc > 2:644113/1‑143 (MQ=255) cAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAgctgct > 2:298612/1‑143 (MQ=255) agagAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGTCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCg > 2:367577/1‑143 (MQ=255) gagaACGTATACAACTACATAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCgg < 1:96925/143‑1 (MQ=255) cGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCg < 1:542814/143‑1 (MQ=255) cGTTTACTACTACACAACTACGCCGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCg < 1:309806/143‑1 (MQ=255) actacACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATc < 1:566265/143‑1 (MQ=255) || TCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATC > NC_000913/124948‑125221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |