Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 125,085 Δ1 bp 100% coding (2069/2664 nt) aceE → pyruvate dehydrogenase E1 component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913125,0850T.100.0% 191.7 / NA 43coding (2069/2664 nt)aceEpyruvate dehydrogenase E1 component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (17/26);  total (17/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTC  >  NC_000913/124954‑125211
                                                                                                                                   |                                                                                                                              
ccACATTCAGTCGCTGCCTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGaa                                                                                                                    <  2:113535/143‑1 (MQ=255)
acacaTTCAGTCGCAGCCTATGCTGCACTTTATCTCTTACTGCCCGGCTTACGCGAACGAAGTTGCTGGCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGaa                                                                                                                    <  1:158304/142‑1 (MQ=255)
  acaTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTGTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGGACg                                                                                                                  >  1:167331/1‑143 (MQ=255)
   caTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGa                                                                                                                 <  1:492569/143‑1 (MQ=255)
      tCAGTGGCTGGCTATCCCGAACTGCATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CCCTACGCTGAACGaaaa                                                                                                              <  2:375573/143‑1 (MQ=255)
      tCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGaaaa                                                                                                              <  1:703010/143‑1 (MQ=255)
      tCAGTCGCTGACTATCCCGAACCGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGaaaa                                                                                                              <  1:303468/143‑1 (MQ=255)
                aCTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTGCcacagg                                                                                                    >  1:180529/1‑141 (MQ=255)
                  tATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAGAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAGCGAACACTACCACCTGcc                                                                                                  >  1:10847/1‑143 (MQ=255)
                       cGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCaa                                                                                             >  2:294937/1‑143 (MQ=255)
                       cGAACTGTATCTCTTACCACCCGGCCTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCaa                                                                                             <  1:713518/143‑1 (MQ=255)
                       cGAACTGTATCGCTTACGACCCGGCTTACGCTTACGAAGTTGCGGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCaa                                                                                             <  1:792277/143‑1 (MQ=255)
                               aTCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGaa                                                                                     <  2:578791/143‑1 (MQ=255)
                                  tctTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGt                                                                                  >  1:82551/1‑143 (MQ=255)
                                        gACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCCGGCACAATGGCACGTGGATACACCAGGGAACGGTTACTACTACA‑CACTACGCTGAACGACAACTACCACATGCCCTCAATGCCCGCAGGTGCTGAg                                                                            >  2:391589/1‑143 (MQ=255)
                                          cccGGCTTACGCTTACGAAGTGGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGa                                                                          <  1:753854/143‑1 (MQ=255)
                                           cctgtttacgcttatgAAGTTGCTGTCATCATGCATGACGGTCTGGAGAGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGaa                                                                         <  1:566171/138‑1 (MQ=255)
                                            cGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAg                                                                        <  1:427978/143‑1 (MQ=255)
                                                ttacgcttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTAt                                                                    <  2:231098/143‑1 (MQ=255)
                                                ttacgcgtacgAAGTTGCGGGCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTAt                                                                    <  2:230332/143‑1 (MQ=255)
                                                  tcgcttatgATGTTTCTGTCATCATGCATGACGGCCTGGAGCGAATGGACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATcc                                                                  <  1:829136/142‑1 (MQ=255)
                                                    gctgacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGt                                                                <  1:732048/143‑1 (MQ=255)
                                                        gcgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAg                                                            <  2:94927/142‑1 (MQ=255)
                                                          gAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGGTGGTGCTGTGGAAGGTATCCGTCAAGGt                                                          >  1:259526/1‑143 (MQ=255)
                                                           aaGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTa                                                         <  1:45822/143‑1 (MQ=255)
                                                                     catcatGCATGACTGTCTGGATCGTATGTACCGTGAAAACCAAGAGAACGGTTACTACGACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACt                                               <  1:48496/143‑1 (MQ=255)
                                                                      atcatGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTc                                              >  1:347317/1‑143 (MQ=255)
                                                                          tgcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGaaa                                          <  1:294937/143‑1 (MQ=255)
                                                                          tgcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCAGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGaaa                                          <  1:132181/143‑1 (MQ=255)
                                                                           gcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACGACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACGCGAAAc                                         >  2:715558/1‑143 (MQ=255)
                                                                                 cGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACACGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTTTCCGTAAAGGTATCTACAAACTCGAAACTATTGa                                   <  1:816877/143‑1 (MQ=255)
                                                                                     cTGGAGCGCATCGACGGTGAAAAACAAAAGAACGTTTACTACTACA‑CACTACGCCGAACGAAAACTACCACATGCCGGCAATGCCGGAATGGGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGt                               <  1:799773/143‑1 (MQ=255)
                                                                                      tGGAGCGTAGGTACGGGGAAAGACAAGAGAACGTTTCATGCTACA‑CTCTAGGCGGAACGAAAACTACCACATGCCGGCTATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTa                              <  2:817757/143‑1 (MQ=255)
                                                                                         aGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTCAAGCTACCTACAAACTCGACACGATTGAAGGTAGCa                           >  2:37692/1‑143 (MQ=255)
                                                                                           cGTATGTACGGTGAAAAGCAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTAACCCATGCCGGCAATGCCGGAAGCTGCTGAGGAAGGTATCCGTAAAGCTATCTACAAACTCGAAACCATTGAAGGTAGCaaa                         <  1:189204/143‑1 (MQ=255)
                                                                                           cGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTACAGGTCTCTACACACTCGAAACTATTGAAGGTAGCaaa                         >  1:378191/1‑143 (MQ=255)
                                                                                           cGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAATGTATCTACAAACTCGAAACTATTGAAGGTAGCaaa                         >  1:572177/1‑143 (MQ=255)
                                                                                             tATGTACGGTGAAAAACAAGACAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAgg                       <  1:328352/143‑1 (MQ=255)
                                                                                                          aaaCAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAgctg          >  2:156716/1‑143 (MQ=255)
                                                                                                          aaaCAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAgctg          >  1:597050/1‑143 (MQ=255)
                                                                                                                gagaACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCTGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGc    >  1:442117/1‑143 (MQ=255)
                                                                                                                gagaACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAACGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGc    >  2:735974/1‑143 (MQ=255)
                                                                                                                  gaACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACGATTGAAGCTAGCAAAGGGAAAGATCAGCTGCACGGCTc  >  2:75290/1‑143 (MQ=255)
                                                                                                                                   |                                                                                                                              
CCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTC  >  NC_000913/124954‑125211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑