Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	100%	intergenic (‑271/‑264)	insA‑5 ← / → uspC	IS1 protein InsA/universal stress protein C

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	34 (0.750)	27/264	NT	100%	intergenic (‑271/‑267)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	32 (0.700)	28/264	NT	100%	intergenic (‑274/‑264)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

TCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1979621‑1979486 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  >  NC_000913/2101749‑2101890                                                                                                                                                                                                                                                                                         TCCCGGTGATCATATAATTTCAAATGCAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAACTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGT                                                                                                                                          >  2:845819/1‑143            ATATCATTTCAAATCTAATTATAAGAAAATAGTGGACAGAATTGCCTGGTTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCG                                                                                                                               <  1:197836/143‑1                           TAATGATAAGAAAATAGTGTACGGATACTTCGTATTAAACTAGTGGTTATTATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCG                                                                                                               <  2:834646/143‑1                              TTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCT                                                                                                            >  2:219624/1‑143                              TTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCT                                                                                                            >  1:403528/1‑143                                    GAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCA                                                                                                      <  2:880511/143‑1                                                     TCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCA                                                                                     <  1:528202/143‑1                                                        CCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGT                                                                                  <  1:623287/143‑1                                                         CGCATTAAACAAGTGGTTAATATATGTAAAACGTTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCCTTTCTTCATGTT                                                                                 >  2:461655/1‑143                                                         CGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AGATTCTTCTCGGCTGACGCAGTCATTTCATTACTTCATGTT                                                                                 >  2:717636/1‑143                                                         CGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTT                                                                                 >  2:193908/1‑143                                                          GCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTT                                                                                >  2:361451/1‑143                                                           CATTAAACAAGTGGTTAATATATGTAAAACATTTCTTTCAAATGAATTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTG                                                                               <  2:181961/143‑1                                                            ATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTATTTGTATTTGGTGAGGGAAGGTGCGAATAAGCGTGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGA                                                                              <  1:288346/143‑1                                                                      tgaTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTT                                                                    <  2:448739/140‑1                                                                                    AAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGC                                                      >  2:733947/1‑143                                                                                        CATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTGGTAATTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTTTTCTCGGCTGACTCAGTCAACTCATTTATTAATGTTTGAGCCGATTTGTGCTCCCGTGACTGCCTTa                                                  >  2:695504/1‑142                                                                                         ATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGA                                                 <  1:438204/143‑1                                                                                              ATTCACATTAATTGGTCTGTGGCCTTTCTTTGTATTTAATTAGGGAAGGTGCTAATAAGCGGGG‑AAATTCTTCTCGGCTGTCTCCGTCATTTCATTTCTTCGTGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAGCAG                                            <  2:425653/143‑1                                                                                              ATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAG                                            <  2:757533/143‑1                                                                                               TTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAAATTCTTCTCGGCTGACTGAGTTGTTCCACACCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAG                                            >  1:405028/1‑143                                                                                                    ATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGGGCCGATTTTTTCTCCCGTAAATGTCTTGAATCAGCCTATT                                      >  1:386216/1‑143                                                                                                      TAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AACTTCTTCTCGGCTGACTCAGTCATGTTATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGTCTTGATTCAGCCTATTTA                                    >  2:769221/1‑143                                                                                                             ATTGTTCCCTTTCTTTGTATTTAATTAGGGAAGGGGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTT                             <  1:382946/143‑1                                                                                                              TTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTT                            <  1:514129/143‑1                                                                                                                     CTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGATTGAGCCGATCTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCC                     >  2:239722/1‑143                                                                                                                      TTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCATATTTAGACCGTTACCTCGACA                    >  2:26296/1‑143                                                                                                                          TTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTA                >  2:391932/1‑143                                                                                                                           TTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTGGCCATTTAc               >  2:751327/1‑142                                                                                                                           TTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAA               <  1:93656/143‑1                                                                                                                           TTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAA               <  1:90502/143‑1                                                                                                                              TATTTAATTAGGGAAGGTGCGAATAACCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTCGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGC            <  1:642068/143‑1                                                                                                                                   AATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTCAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTAAT       >  1:338706/1‑143                                                                                                                                   AATTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTCCTCCCGTAAATGCCTTGAATCAGCCGATTTCGACCGTTCCTTCGCCATTCAAGGCGTTAT       >  2:81925/1‑143                                                                                                                                     TTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCCTTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATCCCTTGAATTCACCTAGTTAGACCGTTTCTTCCCGATTTACGGCatccgcg     >  2:337240/1‑136                                                                                                                                     TTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCC     >  1:553718/1‑143                                                                                                                                     TTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATCCCTTGAAGCAGCCTATTTAGACCGGTTTTTCGCCCTTGAAGGCGGTATCC     >  1:502740/1‑143                                                                                                                                      tgGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGTGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCC    <  2:105355/141‑1                                                                                                                                      TAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCC    >  1:240071/1‑143                                                                                                                                      TAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCC    >  2:679640/1‑143                                                                                                                                       AGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAACTGCCTTGAATCAGCCTATTTAGACCGTCTCTTCGCCATTTAAGGCGTTATCCCa   >  2:393030/1‑142                                                                                                                                       AGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCC   >  2:560861/1‑143                                                                                                                                       AGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATGTAGACCGTTTCTTCGCCATTTAAGGCGTTACCCCC   >  2:751376/1‑143                                                                                                                                        GGGAAGGTGCGAATAAGCGGGG‑AAGTTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAACCTATTTAGACCGTTTCTTCGCCATTTGAGGCGTTATCCCCA  >  2:776842/1‑143                                                                                                                                        GGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  >  1:270680/1‑143                                                                                                                                        GGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  >  1:146103/1‑143                                                                                                                                        GGGAAGGGGCGAATAAGCGGGG‑AGATGCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  <  2:561554/143‑1                                                                                                                                                                                                                                                                                         TCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1979621‑1979486 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  >  NC_000913/2101749‑2101890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.