| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 76,805 | T→A | 100% | E165D (GAA→GAT) | sgrR ← | DNA‑binding transcriptional dual regulator SgrR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 76,805 | 0 | T | A | 100.0% | 87.1 / ‑6.7 | 35 | E165D (GAA→GAT) | sgrR | DNA‑binding transcriptional dual regulator SgrR |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (18/16); minor base C (0/1); total (18/17) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGC > NC_000913/76665‑76943 | ggCAGCGTATTGATTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCCGTGGTGGGCGATGTCTGCATc > 1:632880/1‑143 (MQ=255)ggCAACGTATTGATTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATc < 1:362185/143‑1 (MQ=255) cGTATTGATTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGAGATGTCTGCATCCAGtt > 1:417043/1‑143 (MQ=255) tATTGAGTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTcc < 1:768345/143‑1 (MQ=255) ttCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCAttt > 1:720664/1‑143 (MQ=255) ggCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATgc < 1:803912/143‑1 (MQ=255) gATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCCAAAAGACACGCCAGTCAAGCGGTGAAATTTGCTGCCAGCGGTGGGCGATGTCTGCATCCAGTCCCCGCTTTCCCTCACCTATCcgcg > 2:398717/1‑143 (MQ=255) caTCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGACATCTCTTGCAAGCGGTGAAATTTGCCGCCAGTGATGGCCCATGTACGCATCCCGTACCCAACCTTCCTCATTCATCCGGGTTCGc > 2:147301/1‑143 (MQ=255) caTCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGc > 1:130183/1‑143 (MQ=255) caTCGTCCAGTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGc < 2:685718/143‑1 (MQ=255) cAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCACCCTTTTCCTCATTTATGCGCGTTAGCGAACTGAAGAttt > 1:836895/1‑143 (MQ=255) cGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGc < 1:604926/143‑1 (MQ=255) acgctGGTGAAAATGGACTCCTGGACGCAAAAAGGAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGa < 2:514020/139‑1 (MQ=255) cATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGGACTGCAGATTTGCCGGGCGata > 1:504483/1‑143 (MQ=255) aaTGGACTCCTGGACGCAAACAGCAACGCTGGTGAAAAAGTGCATCGTGGGGCTAGTGGTGGGCGCTGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCg < 1:641471/143‑1 (MQ=255) aCTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACg > 2:420234/1‑143 (MQ=255) cTGGACGCAAAAAGGAACGCCAGTGAAGCGGTGATATTTGTTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCAGTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGgcgc < 1:771147/143‑1 (MQ=255) tGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCa > 1:656970/1‑143 (MQ=255) ggaaagaGAAGCGCCGGTGAGGCGGTGAAATTTGCTGCGAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATgcg < 2:677610/141‑1 (MQ=255) aaaaaGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAAGTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAGTGCGCt > 2:409839/1‑143 (MQ=255) aaaaaGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGCTTTGCCGGGCGATATGGGTTTCGGAACGGCGCCATGCGCt > 1:305228/1‑143 (MQ=255) aaaaaGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGAATACGGAAAGAAGCAAAgcgat > 1:259650/1‑141 (MQ=255) aaaaaGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGACTGCATCCAATTCCCCATTCTCCGCATTTATGCGCGTTAGCGACCTGCCGATTTGCCGGGCGACGGGGGTGCCGGACCGCCGCAACgcgcg > 2:862801/1‑142 (MQ=255) aaaCGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCGCCATTTTCTTCATTTATGCGCGTTAGCGAACTGAAGATCTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAgg > 2:36107/1‑143 (MQ=255) ccAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTGAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCATGTAGCa < 2:676205/143‑1 (MQ=255) catgtcaGCGGTGGAGCTTGCTGCCAGGGGTGGGCGATGTCTGCCTCCAGTTCCCCTTTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAg < 2:302220/137‑1 (MQ=255) gAAGGGGTGAGGTTTGCTGCGAGTGGTGGGCGAGGGCTGCATCCAGTTCCCCATTTTCCTCATGTATGCGCGTTAGCGAACCGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAAGGCGCTGCCAGGTAGCAGATTa < 2:669819/143‑1 (MQ=255) tgcCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGAtagta > 1:82478/1‑143 (MQ=255) tagttttggGCGATGTATGTATCAAGTTACCAATTTGCCGCAAGAAGGCGCGGGAGGGAACTGAAGATTGGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTagag < 1:23808/137‑1 (MQ=255) cAGGGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTagag < 1:437122/143‑1 (MQ=255) cAGCGGTGGGCGATGCCTGCATCCAGTTCCCCATTTTCCTCATTCATGCACGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTagag < 2:720664/143‑1 (MQ=255) cGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAgg < 1:420234/143‑1 (MQ=255) cGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAgg < 1:360500/143‑1 (MQ=255) cTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAGGATTTGCCGCGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCCGGTAGCAGATTACGCACCGGACGAGGGTAGCACACGCGCACCATGTgc > 2:62852/1‑143 (MQ=255) cTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCCAACTGACGATTTGCCGGGCGATATCGGTTTCGGAACAGCGCAATGCGCTGCCAGGTAGCAGATCACGCAACGGACGATAGCAGAGCACGCGCAGGATGTgc > 2:717051/1‑143 (MQ=255) | GGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGC > NC_000913/76665‑76943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |