| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,638,039 | C→T | 10.3% | G24E (GGA→GAA) | ynfN ← | Qin prophage; protein YnfN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,638,039 | 0 | C | T | 10.3% | 103.2 / 2.3 | 46 | G24E (GGA→GAA) | ynfN | Qin prophage; protein YnfN |
| Reads supporting (aligned to +/- strand): ref base C (22/19); new base T (2/3); total (24/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
TTTACACAAACAGACAAAGAGAATTTTCCTGAATTATCAATGCAATAGCATCAAATCAACTCAAGAGCCTTATTGCTGCTTCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTT > NC_000913/1637899‑1638180 | ttgacacAGACAGACAAAGAGAATTTTCCTGAATTACCAATGCAATAGCATCAAATCAACTCAAGAGCCTTATTGCTGCTTCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCg < 1:542825/140‑1 (MQ=255) aaaCAGACAAAGAGAATTTTCCTGAATTATCAATGCAATAGCATCAAATCAACTCAAGAGCCTTATTGCTGCTTCCAGAATTTCTTCTGAAGTAACATGTCGATCCGAGGCTACATAAATGACTTAATGATCCCCGGTCcgag > 2:291624/1‑143 (MQ=255) gACAAAGAGAATTTTCCTGAATTATCAATGCAATAGCATCAAATCAACTCAAGAGCCTTATTGCTGCTTCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGa > 1:631891/1‑143 (MQ=255) aaGAGAATTTTCCTGAATTATCAATGCAATAGCATCAAATCAACTCAAGAGCCTTATTGCTGCTTCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATAAATGACTCTAGGACTTCCGTTCAGAGATGCAAAcc > 2:7776/1‑143 (MQ=255) aaTTTTCCTGAATTATCAATGCAATAGCATCAAATCAACTCAAGAGCCTTATTGCTGCTTCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTAATGATCTCCGGTCAGAGATGGAAACCCTGCg > 1:535250/1‑143 (MQ=255) aaTTTTCCTGAATGATCAATGCAATAGCATCCAATCACCCCAAGAGCCTTATTGCTGCTTCCAGAATTTCTTCTGAAGTAACATATCGATCCCCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCg < 1:606185/143‑1 (MQ=255) cccTGAATGATCAATGCAATAGCATCAAATCACCGCAAGAGCCTTATTGCTGCTTCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCAt < 1:216302/142‑1 (MQ=255) cTGAATTATCAATGCAATAGCATCAAATCCACTCAAGAGCCTTATTGCTGCTTCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTa < 2:273739/143‑1 (MQ=255) gAATTATCAATGCAATAGCATCAAATCAACTCAAGAGCCTTATTGCTGCGTCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACa < 1:738252/143‑1 (MQ=255) tCAATGCAATAGCATCAAATCAACTCAAGAGCCTTATTGCTGCTTCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAgg > 2:48551/1‑143 (MQ=255) aaTGCAATAGCATCAAATCAACTCAAGAGCCTTATTGCTGCTTCCAGAATTTCTTCTGAAGTAACGTGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTGCAggagggcg > 1:114176/1‑136 (MQ=255) gaaTAGCATCAAATCAACTCAAGAGCCTTATTGCTGCTGCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGtt < 1:654972/142‑1 (MQ=255) aaTAGCATCAAATCAACTCAAGAGCCTTATTGCTGCTTCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGgtttgttt > 2:655935/1‑143 (MQ=255) tAGCATCAAATCAACTCAAGAGCCTTATTGCTGCTTCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATACATGACTTTATGATCTCCGGTCAGCGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGttttt > 2:279729/1‑143 (MQ=255) gCATCAAATCAACTCGAGAGTCGTATTGCTGCTTCTATAATTTCTTCTGAAGTAACATGTCGATCTGCGGCTACATAAATGACTTTATGATCTCCGGTCATAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTc < 2:132349/143‑1 (MQ=255) gCATCAAATCAACTCAAGAGCCTTATTGCTGCTTCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATAAATGTCTTTATGATTTTCGGTAAGAGATCGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTc < 1:393775/143‑1 (MQ=255) atcaaCTCAAGAGCCTTATTGCTGCTTCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCGTTACAGTAAGGTGTGTTTTTTCGCCAttt > 1:789438/1‑143 (MQ=255) tCAAGAGCCTTATTGCTGCTTCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGatat > 1:478771/1‑143 (MQ=255) gagCCTTGCTGCTGCGTCCAGAATTGCTTCTGAAGTAACGTGCCGATCCGCGGCTACATAAATGACTGTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCAc < 2:46978/143‑1 (MQ=255) ttATTGCTGCTTCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGAAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCatg > 2:593119/1‑143 (MQ=255) gctgctTCCAGAGTTTCTGATGAAGTAACAGGTCGATCCGCCGCTACATAAATTGCTGTATCGTCTCCACCCACCTCCGGATACCCTGCGGCCATCACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGgt < 2:220495/143‑1 (MQ=255) ctCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTaa < 1:529745/142‑1 (MQ=255) ttctGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCCTCg > 1:724811/1‑143 (MQ=255) ttctGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCg > 1:267593/1‑143 (MQ=255) ttctGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATCAAATCCGGACAAGGATGGATACAATGAGCAGAGAACAGTAAGGTGTGTTATTTCGCCATTTGGAAATTCACGCATGATGGTGTTAACTCCAGTCATCg > 1:408448/1‑143 (MQ=255) gAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTgg > 1:489965/1‑143 (MQ=255) aaGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGc < 1:863239/143‑1 (MQ=255) aaGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGc > 1:671441/1‑143 (MQ=255) aTGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGACAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGGGTTTGTTCGCCATTTGGATAGTCACGCATGCTGGTGGTACCTCCGGTCATCGCTGGCACTAGCa > 1:360961/1‑143 (MQ=255) aTGGCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGTCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCa < 1:460295/143‑1 (MQ=255) ctgtCCGCGGCTACATAACTGACTTTATTGTCGCCGGTCTGAGATGGAAACCCTGCGGCCATTACAGTAAGTTGTGTTTTTTCGCCATTTGGATATACACGCATCCCGCCTTCTACTCCAGTCATCGCTGGCACTACCActgc < 2:272092/140‑1 (MQ=255) aTCCCCGGCTACATAAATGACTTTATGCTCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCActgctg < 2:476400/143‑1 (MQ=255) tCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTgg > 2:351012/1‑143 (MQ=255) tCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTgg < 2:200417/143‑1 (MQ=255) ccgGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTc < 1:419086/142‑1 (MQ=255) cgGCTACATAAATGACTTTATGATCTTCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTAc < 1:112108/130‑1 (MQ=255) cgGCTACATAAATGACTTTATGATCTTCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTAc > 2:112108/1‑130 (MQ=255) cgGCTACATAAATGACTTTATGATCTTCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTAc > 2:106287/1‑130 (MQ=255) cgGCTACATAAATGACTTTATGATCTTCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTAc < 1:106287/130‑1 (MQ=255) gttACATTAATTACTTTATGATCTCCGGTCATAGATGGAAACCCGGCGTCCATTACAGTAACGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCCCTGGCACTACCACTGCTGGTTCaga < 2:133184/141‑1 (MQ=255) gCTACATAAATCACTTTATGATCTCCGGTCAGAGCTGGAAACCCTGTGGCCATTACAGTAAGGTGTGTTTGTTCGCCATTTGGATATTCACGCATGATGGTGTTAAGTGCAGTGATCGCTGGCACTACCACTGCTGGTTCaga < 2:106269/143‑1 (MQ=255) cATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTa > 1:215445/1‑143 (MQ=255) aTGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGGTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTaaaaaa > 1:751811/1‑143 (MQ=255) tGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTaaaaaaa < 1:48551/143‑1 (MQ=255) tttATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAACAACACTAt > 1:310324/1‑143 (MQ=255) ttATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCTCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATg > 1:481299/1‑143 (MQ=255) tcCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGAttttttt < 1:183749/143‑1 (MQ=255) tcCGGTCAGAGATGAAAACCCTGCGACCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTATTAACTCCAGTCATCGCTGGCACTACTACTGCTGGTTCAGAGTTAAAAAAACTATGAttttttt > 2:61101/1‑143 (MQ=255) tcCGGACAGAGATGCAGGCTCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGAttttttt < 1:323157/143‑1 (MQ=255) | TTTACACAAACAGACAAAGAGAATTTTCCTGAATTATCAATGCAATAGCATCAAATCAACTCAAGAGCCTTATTGCTGCTTCCAGAATTTCTTCTGAAGTAACATGTCGATCCGCGGCTACATAAATGACTTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTT > NC_000913/1637899‑1638180 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |