| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | 100% | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 100.0% | 88.3 / ‑6.7 | 29 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (8/20); total (9/20) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGG > NC_000913/2132652‑2132913 | tGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAGTTTGTCTTTGTGCTTGCGGTAAAAGTTGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGt < 1:753756/143‑1 (MQ=255) gggaaaCAGCCGATATTTTTTGCTGGCGCCGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCTTTTCTCCGGGATTGATATGCAGGCTCTGCGTCGCCtctt < 1:456258/140‑1 (MQ=255) gTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttc < 1:155613/143‑1 (MQ=255) gTAAACAGCTGATATTTTTTGCTGGCGCAGTAGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCAACGAGATTGATAGGCAGGCTCGGCGTCGCctcata > 2:737098/1‑140 (MQ=255) aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATGGATATGCAGGCCCTGCGTCGCCTCTTTTACt > 1:851883/1‑143 (MQ=255) gttatTTTTTGCTGCTGCGGTCGAATTTGTCTTTTTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCAc < 2:144956/141‑1 (MQ=255) tttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTc < 2:747907/143‑1 (MQ=255) ggCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTAGCCTCTTCTACTACCCACGGTTCGCCGTACt > 2:524321/1‑143 (MQ=255) gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTCCTCCTTTCCCCGGTTGGCCGTACTccacaacca > 1:238293/1‑143 (MQ=255) aTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCAGGGTTCGCCGTAGTCCACCGCCATc > 1:749982/1‑143 (MQ=255) tGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGc < 2:830351/143‑1 (MQ=255) tttGTTCTTGCGGTAAAACTGGAAGTACCCGGAGAATTTTTTCGTTGACGACGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCCCCAACACTCAACAATTTCACGTACTCCACCACCATCCGCAGGa < 1:334151/143‑1 (MQ=255) gCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCTGGAAGATg > 1:108094/1‑143 (MQ=255) tggCGGTACCAGTGGGAGGGCGCGGCGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc < 1:643314/141‑1 (MQ=255) tgaggTAAACGTGGAAGTGTCCGGACTGTTTCTACCGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCg < 1:790957/140‑1 (MQ=255) ggTAAGAGCGGAAGTACGCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTaa < 1:8236/143‑1 (MQ=255) ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCGACGGTTCGCCGTACTCCACCACCATCCGCAGGGAGGTGTCGtcg > 1:351282/1‑141 (MQ=255) ggtggccgTACCTGGAGCGTTTTTGCGGTGACGAGGTGATCTGCATTTCACTGTGATTGATATGCCGGCTCTGCGTCGCCTCTTCTACTTTCCGCGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt < 2:469017/136‑1 (MQ=255) aggactcGGAGAATTTTTTCGGTGACGAGGTGATCTCCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCGCGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCt < 2:133667/137‑1 (MQ=255) agaATGTTTTCGGAGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg < 1:162081/143‑1 (MQ=255) taaTTTTTTCGGTGACGAGGTGATCTGCCTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTGCTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGTCGCTTTCAGTTCgg < 2:190500/142‑1 (MQ=255) aggttttgCGGTGGCGGGGTGAGCTGCATTGCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCGCGGTGCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGt < 2:425895/140‑1 (MQ=255) ttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa < 1:136012/143‑1 (MQ=255) ttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa < 1:847412/143‑1 (MQ=255) tCACCGTGGTTGAGATGCGGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGc < 2:327647/143‑1 (MQ=255) ccGTGATTGATATGCATGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCa < 2:556963/143‑1 (MQ=255) cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAGCGCCATGCCTAg > 1:30902/1‑143 (MQ=255) cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg > 2:91070/1‑143 (MQ=255) gggattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAgg < 1:276300/141‑1 (MQ=255) | TGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGG > NC_000913/2132652‑2132913 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |