| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 87,481 | C→A | 100% | T42K (ACA→AAA) | ilvH → | acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 87,481 | 0 | C | A | 96.7% | 81.8 / ‑3.8 | 35 | T42K (ACA→AAA) | ilvH | acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base A (13/19); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCGGGAAATCATGCTG > NC_000913/87349‑87620 | agcTGATTAGGCGCCGGATGTTACCCGTCTTACTCGAGAATGAATCAGGCGCGGTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGt < 2:246681/141‑1 (MQ=255) ccgggttATGCGCCGGATATTATCAGTCGTGCTCGAGAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTGTGTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTa < 2:309540/138‑1 (MQ=255) tGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTAAAGCATTGAAAGCCTGACCGTTGCGCCAACCGACGATCAGAAATTTCCGCGTagg > 2:256959/1‑141 (MQ=255) gATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGa > 1:435831/1‑143 (MQ=255) tGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGCTCCGAAATTATCGCGTATGACCCTc > 1:719352/1‑143 (MQ=255) aGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGCCCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATg < 1:43829/143‑1 (MQ=255) gTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTCCAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGCGGGCGATGa > 1:817264/1‑143 (MQ=255) cTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTTACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGaaa < 1:701117/143‑1 (MQ=255) tACTGGAAAATGAAGCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGaaaaa < 1:863025/143‑1 (MQ=255) tACTCCAAAATGAACCAGGCGCGTTATCCCGCCTGATTGGCCTTTTTTCCCAGCGTGGCTACACCATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGaaaaa < 1:712826/143‑1 (MQ=255) tCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCCGGCGACGAGTCGAAGTTATCGCGTATGACGATCCAGACCGCGGGCGATGAAAACGtg > 2:307134/1‑142 (MQ=255) gAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGCCCATCCAGACCGTGGGCGATGACAACGTCCt > 1:524771/1‑143 (MQ=255) gAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACt > 1:442912/1‑143 (MQ=255) aaaTGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAACGCTTGATCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGCGGGCGATGAAACAGAGCTTg > 2:773654/1‑143 (MQ=255) gcgTTATCCCGCGTGATTGGCCTTTTTGCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGaaaa < 1:507832/143‑1 (MQ=255) aTCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTGGAGCAGATAGAAAAGCAAt > 1:567389/1‑143 (MQ=255) ccGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTac < 2:313489/143‑1 (MQ=255) ttctCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGt < 1:667517/140‑1 (MQ=255) tCCCAGCGTGGCCACAACATTGAACGTCTGACCGTTGCGCCCACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCtt < 2:144248/143‑1 (MQ=255) ccAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAACAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTgc > 1:192551/1‑143 (MQ=255) aacaTTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTAGTAGCGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGtgagtgaggt > 1:501935/1‑141 (MQ=255) caTTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTgg < 1:575279/143‑1 (MQ=255) ggcaGCCGGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGCGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCa < 1:24304/140‑1 (MQ=255) ccTGACCGTTGCGCTAACCGACGATGCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATAGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGgcg < 2:438605/143‑1 (MQ=255) gACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACATGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGgcgcgc > 1:506557/1‑143 (MQ=255) gACCGTTGCGCCAACCGACGAACCGAAATTATCGCGTATGACCATCCAGTGGGTGGGCGAACAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGgcgcgc < 1:405683/143‑1 (MQ=255) ccGTCGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCAt < 2:46990/143‑1 (MQ=255) cGTTGCGCCGACCCAGCATGCGAAATTAGCGCGTATGACCATCCAGACCGTGGCTGATGAAAAAGTAGTTGCGCTGATCGCAAAGCAATTACACATACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATg < 2:770838/143‑1 (MQ=255) gTTGCGCCAGCCGACGATCGGAAAGTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGt < 2:212834/143‑1 (MQ=255) cgtGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGACGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGt < 2:451168/141‑1 (MQ=255) gcCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGAACGAAAAGCAATTACACAAACTGGTCGATGTCTTGGGCGTGAGTGAGTTGGGGCACGGCGCGCAGGTTGAGc > 2:157885/1‑143 (MQ=255) cgacgaTCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGAGAGTTGGGGCAGGGCGCGCATGTTGAGCGGGaaa > 1:752001/1‑143 (MQ=255) aTCCGAGATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGCGTGAGTTGGGGCAGGGCGCGCATGTTGAGCGGGACATCATg > 1:272065/1‑143 (MQ=255) aTCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCGGGAAATCATg < 1:773654/143‑1 (MQ=255) cGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGGCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTCGAGATTGAAATCATGCTg > 2:3279/1‑143 (MQ=255) | ACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCGGGAAATCATGCTG > NC_000913/87349‑87620 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |