BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,041,398	T→G	100%	L29R (CTT→CGT)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,398	0	T	G	100.0%	101.6 / NA	34	L29R (CTT→CGT)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (18/16); total (18/16)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACG  >  NC_000913/3041260‑3041533

cgCTTTTTTTCTATTAGTCATTAGTACGCCTTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGcc                                                                                                                                     <  1:705951/142‑1 (MQ=255)

     ttttACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCCGATGACGATTGGTGACCGGGCCCGTGccaccct                                                                                                                                >  1:786717/1‑141 (MQ=255)

         accgcgtgTCATCAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTGTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACt                                                                                                                            <  1:24979/136‑1 (MQ=255)

          cccttAGTCATTGATACGCCGTCACGCAACTCAGGACTCTATTATGGCTTTTACACCTTTCCCTCCCCTTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTg                                                                                                                           <  2:757800/140‑1 (MQ=255)

               aGTCATTAATACGCCGGTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGcgcg                                                                                                                      <  2:465106/143‑1 (MQ=255)

                 tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCCTCTGCCCGGTTACCGCTGACGCTGATGCCGCTTGCTGACTGGGCGCGTGCCACCATTACTGGCGCggc                                                                                                                    >  2:560319/1‑142 (MQ=255)

                   aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACa                                                                                                                  >  1:374766/1‑143 (MQ=255)

                          cGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACAGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGaaaa                                                                                                           <  2:90412/143‑1 (MQ=255)

                                            taCTCTATTATGGCTTTTTCACCTTTTCCTCCCCGTCATCCTACTGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGt                                                                                         <  1:306159/142‑1 (MQ=255)

                                                 tattatGGCTTTTGCACCTGTGCCTGCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCGGACGCTGATGACGCTGGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcag                                                                                    <  1:173978/143‑1 (MQ=255)

                                                 tattatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGCCGCTTGATGACTGGGCGCGTGCCACCATTACTGGTGCGGACAGCGAAAAATATATGCAGGGTCAGGTGCcag                                                                                    >  2:72213/1‑143 (MQ=255)

                                                 tattatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCACGATTACCGCTGTGGCTGATGACGCTTGATGACTGGGCGCGTGCCACCGTTACTGGCGCGGACAGCGGGAAATAGAGGCGGGGTCAGGTGagag                                                                                    >  1:241948/1‑143 (MQ=255)

                                                        gCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGAACACGCTTGATGACTGGGCGCGTGCCACCCTTACTGGCGCGGGCAGCGAGCAATCTGTGCCGGGTCAGGGGAGAACAGGTgc                                                                             >  2:275860/1‑142 (MQ=255)

                                                                    tttCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCCTTACTGGCACGGACAGCTAAAAATATATGCAGGGTCCGGTGACAGGAGGTGTCAGCCAGAggga                                                                 >  2:768399/1‑142 (MQ=255)

                                                                     gtcctccGCGTCAGCCTGCGGCTTCTGCCCGTTTGCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCa                                                                <  2:579688/142‑1 (MQ=255)

                                                                      tcctccCCGTCGGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGACGGCag                                                               <  2:21543/143‑1 (MQ=255)

                                                                         tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCACTACTGGCGCGGACAGCGAAAAACATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaag                                                            >  2:410697/1‑143 (MQ=255)

                                                                           cccGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCCGCCAGATGGCcgaagct                                                          >  2:452292/1‑141 (MQ=255)

                                                                           cccGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGAt                                                          >  1:749992/1‑143 (MQ=255)

                                                                             cGTCAGCCTAGGTCTTCTGCCCGTGTACCGCGGGCGCGGATGACGCGTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGGCAGCCAGATGGCAGAAGATCa                                                        <  2:701436/143‑1 (MQ=255)

                                                                             cGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACGCGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCCGCTAGATGGCAGAAGGTcc                                                        >  2:165703/1‑142 (MQ=255)

                                                                               tCAGCTTACTGCTTCTTCCCGTTTACCTCTGACGCTGATGACGCTTGAGGACTGGGCGCGTGCCACCATTACGGGCGCGGACAGCGAACAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGACGGCAGAAGATCAGc                                                      <  1:605930/143‑1 (MQ=255)

                                                                                    ccaCGGCTTCGGCCCGCTTACCGCTGACGCGGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTg                                                 <  1:889574/141‑1 (MQ=255)

                                                                                     tACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGCCAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGc                                                >  1:536256/1‑143 (MQ=255)

                                                                                           ttCTGCCCGTTTCCCGCCGACGCTGATGACGCTTGATGCCTGGCAACGAAAGCGCATTTCTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgccg                                          <  1:404449/143‑1 (MQ=255)

                                                                                             cTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCCTTACTGGCGCGGACAGCGAACAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCACATGGCAGAAGATCAGCACCTGCTCgccgcc                                        >  1:504291/1‑143 (MQ=255)

                                                                                                            tgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCaaa                         >  1:745993/1‑143 (MQ=255)

                                                                                                             gacgctgaTGACGCTTGATGACTGGTCTCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTTAGGTGACAGCAGATGTCAGCTAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAg                        <  2:488362/143‑1 (MQ=255)

                                                                                                                   gacgaggCTAGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAGTATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaaaa                  <  2:521499/137‑1 (MQ=255)

                                                                                                                         gCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAGGGTAAAAAGTGGa            >  1:576323/1‑143 (MQ=255)

                                                                                                                         gCTGGAGGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGAGAGGTGTTATCATTGTTCGGCCAGATACCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGa            <  1:649251/143‑1 (MQ=255)

                                                                                                                                   tGGGCGCGTGCCACCATTACTGGCGCGGACAGCGACAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCCCCCATTGCGACGCCAAAGGTACAATGTCGTGCAATTTACg  >  1:281832/1‑143 (MQ=255)

                                                                                                                                   tGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGATCAATTTACg  >  2:556407/1‑143 (MQ=255)

                                                                                                                                   tGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCCGCTCGCCGCCCCTTGCGACGACCAAGGGAACACGTGTAGCAATTTACg  >  2:840968/1‑143 (MQ=255)

AGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACG  >  NC_000913/3041260‑3041533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`