| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,176,209 | C→A | 100% | D209Y (GAT→TAT) | cpdA ← | cAMP phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,176,209 | 0 | C | A | 100.0% | 79.3 / ‑6.7 | 28 | D209Y (GAT→TAT) | cpdA | cAMP phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (11/16); minor base G (0/1); total (11/17) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGAT > NC_000913/3176071‑3176343 | tCAGCGGGCCAGCAGCATGTAACTCGAGCGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGc < 1:661250/143‑1 (MQ=255) tCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGGACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGAGGAAGATGACCGCACAg > 1:828622/1‑143 (MQ=255) ctgCCAGTCGGCCGCGATGCCATCCAGCGTAAAGTTGTAACAGCGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTAtt < 2:594835/141‑1 (MQ=255) cAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGAATTTGAc > 1:227729/1‑143 (MQ=255) cgATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGCTGAATATGCCCGCACAGCAAGCATTTGACGTGCGGAAAc > 1:278315/1‑143 (MQ=255) gTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGc < 2:162838/143‑1 (MQ=255) gAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGCTACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTc > 1:474956/1‑143 (MQ=255) gAACAGTGCGGCGCAATCTGCACACAGGTGGACGGCGTTGCCAGCAGGCGGCGACCATGCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTc < 2:30742/143‑1 (MQ=255) agggCGGCTGAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGccc < 1:558384/140‑1 (MQ=255) tGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCcgc < 1:254496/143‑1 (MQ=255) tGCGGCATAAACGGCACACAGGCCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCcgc < 1:669716/143‑1 (MQ=255) cggcTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGGCCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCa < 2:728675/140‑1 (MQ=255) aaaCTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCa < 1:183649/143‑1 (MQ=255) acaGGTTGACGGCGGTGCCAGCTGGCGGCGACCATTCCAGTCGAGATACAGCGCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGtt < 2:732898/143‑1 (MQ=255) acaGGTCGACGGCGTTGCCAGCAGGCTGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGtt < 1:887047/143‑1 (MQ=255) tgtggccggcggTGCGCGCAGGCGGCGACCATGCCAGTCGAGCTACAGCTCCTGATGAAGATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACTGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGAt < 2:257963/137‑1 (MQ=255) gTCGACGGCGTTGCCAGCAGGCGGCGTAGCTGGTAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCCGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGacc > 2:727634/1‑141 (MQ=255) gTCGACGGCGTTGCCAGCAGGCGGCGAGCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATc < 1:113669/143‑1 (MQ=255) cGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGa < 1:252945/143‑1 (MQ=255) aCGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGc > 1:140765/1‑143 (MQ=255) cGTTGCCAGCGGGCGGCGACCATTCCAGTCGAGATGCAGCTCCTGATGAATATGACCGCACAGCAAGTAGTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAAc < 2:278315/143‑1 (MQ=255) cGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAAc < 1:845893/143‑1 (MQ=255) ccAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACaa > 1:296921/1‑143 (MQ=255) cagcagGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGCGCCAACTACAAc > 1:581544/1‑143 (MQ=255) gcagGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTCGATCGAGCCAACTACAAccc > 1:569042/1‑143 (MQ=255) tgtgctaCCATGCCTGTCGAGTTATAGATCATGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAg < 2:39496/137‑1 (MQ=255) aGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCCAGCCAACTACAACCCGCAGGTAGCGgctgatga > 1:875074/1‑143 (MQ=255) gTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTATAACCCGCAGGTAGCGgatgatgat > 1:858150/1‑143 (MQ=255) | TCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGAT > NC_000913/3176071‑3176343 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |