| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,291,109 | A→T | 11.5% | K766* (AAA→TAA) ‡ | yraJ → | putative fimbrial usher protein YraJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,291,109 | 0 | A | T | 11.5% | 79.1 / 5.2 | 35 | K766* (AAA→TAA) ‡ | yraJ | putative fimbrial usher protein YraJ |
| Reads supporting (aligned to +/- strand): ref base A (10/21); new base T (2/2); total (12/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-02 | |||||||||||
GCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCA > NC_000913/3290976‑3291251 | gCAGCTTCACGATCACGGCGATCTTGAACGACCCACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGc < 1:281683/143‑1 (MQ=255) aGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcga < 2:334987/143‑3 (MQ=255) aaCCTCGATCTTGACCAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCTATCGCAACGGTAAACCGTTGCCCTTCGGCGctct < 2:600436/143‑1 (MQ=255) tttaacaatcaTCTCAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAAc < 1:744747/140‑1 (MQ=255) gAACAAGCAGCACAGTTTGTGGTGCCCGGCAGTGGTGCAATGGTAGCGGCGCGTTTCAGCGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACga < 2:792511/143‑1 (MQ=255) aacagacaACACACGGTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCACTATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgat < 1:343698/143‑1 (MQ=255) acaaacaaCACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACCGTGTAGGGTCCCAACCGTAACCCGACTCCCCTCGGCGCTCTTGCCAGCAACgatg > 2:142625/1‑143 (MQ=255) agacaaccTAGTTTGTGGTGCGCAACAGAGGTGCATTGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCATCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTTTTGCCAGCAACgatgat < 2:24784/141‑1 (MQ=255) cacaGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTGAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgggg < 2:182895/143‑1 (MQ=255) ttGTGGTGCCCAACAGAGGTGTAATGGTAGCGGCGCGTTTCATCTCCAATATTGGTTATCGTGTATTTGTTACAGTCAGCGAGCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGcaacaa < 1:69636/143‑1 (MQ=255) tgtgGTGGCCAGTAGGGGTGCAGGGGGAGCGGCGCGGTTCAACGCCAATATCGGTTGTCGCGTACTTGTTACAGTCAGCGATCGCGAGGGTAAACCGTTGCCCTTTGGCGCGCTTGCCAGCAACGATGATACGGGGCAACaaa < 2:327691/143‑1 (MQ=255) tgtgGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACaaa < 1:113649/143‑1 (MQ=255) tgCCCAGCAGCGGGGCAACGGTAGCGGCGCGTTTCAACGGCAATATCCGTTATCGCGTACTTGTTACAGTCAGCTAGCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAtc < 1:584503/143‑1 (MQ=255) cccAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTCGCCCTTTGGCGCTCTTGCCAGCAACGATCATACGGGCCCACAAAGTaaccg > 2:134539/1‑139 (MQ=255) aaCAGAGGTGCAATGGTAGCGGCGCGTTTAAACGGCAAGATCGGTTATGGCGTACTGGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGa > 2:485749/1‑143 (MQ=255) aCAAAGGTGCAATGGTAGCGGGGCGTTTCAACGCCAATATCGGTTATCGCGGACTTGTTACAGCCAGCGAGCGCAACGCTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGAt < 2:246531/143‑1 (MQ=255) ggagGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGa < 1:774809/142‑1 (MQ=255) cAATGGTAGCGGCGCGTTTCAACGCCAATATTGGTTATCGCGTACTTGTTACAGTCCGCGATAGCATCGGTAAGCCGTTGCCATTTGGTGCTCTTGCCAGCAAGAACGCTACGGGGCACCACAATCTCGTGCATGAGggcggc > 2:818839/1‑143 (MQ=255) cAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCCGCAACGATGATACGGGGCAACACAGTACCGTCGCTGAGggcgga > 2:641269/1‑142 (MQ=255) cAATGGTAGCGGCGCGATTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATGGCAACGGTAAACCGTCGCCGTTTGGCGCTCTTGCCAGCGACGATGAGACGGGGCACCAAGGTCTCGCCGGTGAGgccggg > 2:564770/1‑142 (MQ=255) gcggcgCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtata < 1:809847/143‑1 (MQ=255) ttCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTgg < 1:86234/143‑1 (MQ=255) tccAACGCAAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGTCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTgg < 1:520579/141‑1 (MQ=255) atatCGGTTATCGCGCACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGCCGCTCTTGCCATCAATGATGCTACGGGGCAACAAAGTATCGTCGATGAGCGCGGCATACTATTTCTCGCAGGGTTATCCAGt > 2:822327/1‑143 (MQ=255) gTTATCGCGTACCTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATATGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATca < 1:453140/143‑1 (MQ=255) gTTATCGCGTACCTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGACGATACGGGGCAACACAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGTATATCGAGTAAATca < 1:459961/143‑1 (MQ=255) aCTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGa < 1:338068/143‑1 (MQ=255) ttggtgaCAGTCAGCGATCGCAACGGTAACCCGTTGCGCTTTGGCGCTCTGGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGAc < 1:55784/137‑1 (MQ=255) gcgcTCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGa < 1:512680/143‑1 (MQ=255) gCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAGGCTGGACTGTACGCTGGGGaagtagg > 2:43817/1‑138 (MQ=255) gCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAg > 2:164886/1‑143 (MQ=255) gCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAg < 1:477652/143‑1 (MQ=255) gCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGGAAAGCACAAAGCTGGACTGTACGCTGGGGAAAGCAg > 2:691095/1‑143 (MQ=255) cAACGGTAAACCGTTGCCCTTTGGCGCTCTTGACAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGGGGGCGGCATACTATATCTCTCTGGGATACCGAGTAAATCACAAAGCTGGACTGGACTCGAGGGaaaaaagg > 2:463654/1‑138 (MQ=255) aaCGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGCCCACAAAGTATCGTCGATGAGGGCGGCATACTATAACTCACTGGGATATCGAGTAAAACACAAAGCGGGACAGTACGCGGGGGGCATCACGc > 2:270738/1‑143 (MQ=255) aaaCCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGCTATCGAGTAAATCACAAAGCTGACCTGCACGCTGGCGGAATCAAGCAGATca > 2:123341/1‑143 (MQ=255) | GCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCA > NC_000913/3290976‑3291251 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |