| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,439,589 | G→C | 8.5% | intergenic (‑80/+27) | yhdN ← / ← rplQ | conserved protein YhdN/50S ribosomal subunit protein L17 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,439,589 | 0 | G | C | 8.5% | 111.4 / 3.2 | 44 | intergenic (‑80/+27) | yhdN/rplQ | conserved protein YhdN/50S ribosomal subunit protein L17 |
| Reads supporting (aligned to +/- strand): ref base G (20/20); new base C (2/2); total (22/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01 | |||||||||||
CACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCAC > NC_000913/3439452‑3439731 | cACCTTTCGCTTGCCCTTCTGCTATATTGCGCCCTGACCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCgg < 2:84127/143‑1 (MQ=255)cACCTCTCGCTGGCGTTTCTTCTATATGGCGCTCTGCCCACGGGTGAAGTAACCACATAAATCCTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATAATACGGGTATAAAAAAACCCGCCGGGGCgg < 2:699882/143‑1 (MQ=255) tttCGCTTGCGTTTCTGCTATATGACGGTCTGCCCACTGGTCAAGTAACTACATGAAGAAGGCAGGGGGGGTAGACGAAGAGTACAGATCTTGTAGATAAGGGGGGATAGTACGGGTATAAAAAAAGCCGCCGGGGCgggatg > 2:790043/1‑140 (MQ=255) tttCGCTTGCGCTTCGGCGATATGGCGATCTGCCCACTGGTCAAGTAGCCACATAAATACGCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGttt < 2:378570/143‑1 (MQ=255) ttgcGCTTGCGCTTCTGCGATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACGGCGGTGGTAGGTGAGTGGGGATACTACGGGTATAAAAAAACCCGCCCCGGCGGGttt < 1:83230/140‑1 (MQ=255) gCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCCCGGCGGGttttttt < 1:89400/143‑1 (MQ=255) ccTTGCGCTTCTGCTATATGGCGCCCTGCCCACGGGTCAAGTAACCACATAAACACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGttttttt < 1:769097/142‑1 (MQ=255) gcTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTg > 1:420680/1‑143 (MQ=255) ggCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCAGCCGGGGCGGGTTTGTTTACGTTGATTCAGATGActg > 1:333476/1‑142 (MQ=255) tGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgcagca > 1:235712/1‑143 (MQ=255) tGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGATATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTGTGTACGTTGCTTCAGATTACTCTgcagca > 1:81203/1‑143 (MQ=255) cccACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATCAGTGGGGATACTACGGGTATAAAAACACCCGCCGGGGCGGGCTTTCTTCCGTTGCCTCAGATTACTCCGCggccgc > 2:762623/1‑143 (MQ=255) ggTCAAGTACCCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGc < 1:601566/143‑1 (MQ=255) aGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTtct < 2:257900/143‑1 (MQ=255) gTAACCACATCAGGACTCCAGGGGATGAACAAAAAGAGTACCGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAATCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTtctc < 1:708217/143‑1 (MQ=255) cacaTAATTGCTCCAGGGGATGAACAAAAAGAGTACCGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAAc < 2:806738/143‑1 (MQ=255) cacaTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAAc < 1:180132/143‑1 (MQ=255) acaTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATACGTGGGGATACGACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACg > 1:842033/1‑143 (MQ=255) caTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGa > 1:847462/1‑143 (MQ=255) aTAAATACTCCAGGGGATTAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGCTACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGAt < 2:228271/143‑1 (MQ=255) aCTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCa > 1:684728/1‑143 (MQ=255) tCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGc > 2:86170/1‑143 (MQ=255) cAGGGGATGAACAAAAAGAGTAGAGCTAGTGTAGATAAGTGGCGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTc < 1:366480/143‑1 (MQ=255) cAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGccc > 2:434356/1‑141 (MQ=255) tggggATGAACAAAAAGTGTACAGCTATTGTAGAGAGGGGGGGATACTACGGGTATAAAAACACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCg < 2:87783/142‑1 (MQ=255) tGACCAAAAATAGTACAGCTATTGTAGATAAGTGGGGATACTACCGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTaa < 1:651665/143‑1 (MQ=255) aaCAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGACCAACCAGCTCGATGTAAGc > 2:356040/1‑143 (MQ=255) cAAAAAGAGTACAGCTATTGTAGGTAAGTGGGGATACTACGGGTATAAAACAACCCGCCGGGGCGGGTTTTTGTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTGTCTCTGAACGATCAACCAGCGCGACGTAAGCCa > 2:707158/1‑143 (MQ=255) aaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTGTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTGAGCCAtag > 2:271614/1‑141 (MQ=255) ttGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGCTGTAACCCAACGGCTCGTTGTAGCCt > 1:832457/1‑143 (MQ=255) gATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACg < 2:837552/143‑1 (MQ=255) agggggggCTGCTGCGGGTAGAAACAACCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCGGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGaa < 2:162233/139‑1 (MQ=255) tGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTCAGCGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGTAGcc > 1:862503/1‑143 (MQ=255) gggATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTGGCTTCAGATTACTCGGCAGCAGCTCCTGCTTTCTCTGAACGATCAACCAGCTCGCTGTAACCCATCGGCGCGGGGTCTCCCGAATGGAAGCcac > 2:466649/1‑143 (MQ=255) tgATACTCCGGGTATAGAAGTCCCCGCCGGGGCGGGTTGTTTTACGGTGCTCCAGATTCCTCTCCAGCAGCTTCTGCTCTCTCTCACCGAGCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCcaca < 1:581904/142‑1 (MQ=255) tactacGGGTATAAAAACTCCGGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCAGCGGCGCGTTGTCGCCTGCACGGAAGCCACACtt < 2:293460/143‑1 (MQ=255) tacGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGAGGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGGCACACTTCAg > 1:438444/1‑143 (MQ=255) acgtaAAAAAACCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGACCGATCGACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACGCTTCAGactcc > 2:190234/4‑139 (MQ=255) tataAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGATTCTGCTCTCTCTGAACGATCAACCAGCGCGATGTCAGCCATCGGCGCGTGGTCGCCTGCACGGCAGCCACCCTTCCGCATACg > 2:630077/1‑143 (MQ=255) aaaCCCGCCGGGGCTGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACTATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTaa < 1:583092/143‑1 (MQ=255) agcccGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAac < 1:771264/141‑1 (MQ=255) aaCCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCGCGGAAGCCACACTTCAGCACACGCGGGTAac > 1:474812/1‑143 (MQ=255) cgccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACAAACAACCAACTCAATGTAAGCCATCGGCGCGTTGTCGCCTGCACACCACCCACACTTCAGAATACGATTGTAaccacc > 1:237098/1‑143 (MQ=255) cgGGGCGGGTTTTTTTACGTTGCGTCAGATTACTCTGCAGCAGCTGCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGc < 1:852484/143‑1 (MQ=255) ggggCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCa > 1:296048/1‑143 (MQ=255) gggCGGGTGTGTGGACGTGGCTTCAGAGTACGCTGCAGCAGCTTCTGCTTGCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCAc < 1:173244/143‑1 (MQ=255) | CACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCAC > NC_000913/3439452‑3439731 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |