| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 781,670 | A→T | 9.1% | intergenic (+18/‑415) | lysQ → / → nadA | tRNA‑Lys/quinolinate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 781,670 | 0 | A | T | 9.1% | 110.8 / 5.0 | 53 | intergenic (+18/‑415) | lysQ/nadA | tRNA‑Lys/quinolinate synthase |
| Reads supporting (aligned to +/- strand): ref base A (24/22); new base T (2/3); total (28/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01 | |||||||||||
GCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATG > NC_000913/781531‑781811 | gCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAg < 1:299737/143‑1 (MQ=255) gACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgcgc > 1:382267/1‑143 (MQ=255) aCCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTACAACAGCGCCCTCAACGCGCt > 2:886169/1‑143 (MQ=255) cccACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATGCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtt > 2:544455/1‑143 (MQ=255) ccaccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAACAAGCGCCCTCAAGGCGCttt > 1:501644/1‑143 (MQ=255) ccaccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCttt < 1:753381/143‑1 (MQ=255) ccaacaTCATAATTGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCttt < 1:290542/143‑1 (MQ=255) tCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCt > 1:609079/1‑143 (MQ=255) cgcaaTTATGGCCGCCTAACCCCGGAAGGGGTCGGTAGCTCAGTTGGTAGCGCAGTTGACTTTCAATCAATCGGTCGCAGGTTCGCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAGAGGCGCTTTTTTGCTATCTg < 2:461732/139‑1 (MQ=14) gATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGa < 1:426739/143‑1 (MQ=255) tAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATAc > 2:230668/1‑143 (MQ=255) cagcaTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCaaa > 1:416458/1‑143 (MQ=255) cagcaTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCaaa < 2:336972/143‑1 (MQ=255) tatcaccGCAAGGGGCCGCTAGCTCAGTTGGTAGAGCAGTAGACTTTTAATCAATGGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTc < 2:458234/138‑1 (MQ=11) tAATCCCGCGCGGGGCCGTGAGCTTAGTTTGTAGCGCAGTTGACCCTTTCTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTc < 2:628968/143‑1 (MQ=11) tAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTc < 1:414283/143‑1 (MQ=255) aaTCCCGCGATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCg < 2:157977/143‑1 (MQ=255) cccGCAAGGGGCCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAACGGCGCTTTTTTGCTATCTGCGATACTCACAGCTTCGGAc > 2:432794/1‑143 (MQ=14) gCAGGGGGTCGTGAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGGACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTg < 2:95037/143‑1 (MQ=255) ggggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCCAAGATTCGCACCTgaagc > 2:810986/1‑143 (MQ=12) ggggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGAAACCCAAAGATGCGAACGTgaagc > 2:114520/1‑143 (MQ=11) tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATATAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGt < 1:556788/143‑1 (MQ=17) cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGtt > 1:155682/1‑143 (MQ=32) tAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttga < 1:801227/143‑1 (MQ=32) gCAGTGGACTTTTAATCCATTGGCCGCAGGTTCGAATCCTGCACGACNCACCAATGTAAAAAAGCGCCCTTAAGGCGCTGTTTTGCGATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGaaa < 2:85154/143‑1 (MQ=11) gTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAaacaa < 1:49913/143‑1 (MQ=32) tGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCCGCAGGTTTGAGTTGAGCGCAGCGAAACAACg > 1:83273/1‑143 (MQ=17) ttAATCATTTGGCGGCAGGTCGAAATCCGGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCg < 1:751526/143‑1 (MQ=11) tgggccccAGGTTCGAACCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcggc < 1:728433/136‑1 (MQ=12) gTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTCTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGAc > 1:870452/1‑143 (MQ=17) aGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGccc > 1:139610/1‑143 (MQ=32) ttCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGaa < 1:523757/143‑1 (MQ=32) gAATCCTTCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAggg < 2:410635/143‑1 (MQ=17) gAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTTAAAGCTCCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACACCGGAGCCGCTCGCGGCGACGGCCCGAAcgg > 1:81809/1‑143 (MQ=11) caTCCGGCCCGACCCCGCGATGTAACAAAGCGCCCTAACGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTGGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgc < 2:603371/142‑1 (MQ=11) aaTCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGCAACTCCAAGATTCGAACCTGCAGCAGGTTTGAGCTGACCACAGCGAAACAACGGCGACGCTAGCGGCGCCGGCCCCAACGgc > 2:162019/1‑143 (MQ=11) aaTCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACCCCAAGATCCGAACCTGCCGCCGGTTTGAGTGAAGCACAGAGAAGCCACGGAGCCCCGCGCGGCGAGGGCCCGAGGGgc > 2:159426/1‑143 (MQ=11) aaTCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACCCAAAGATACGATCCTGCAGCACACTTGAGTTGCGCCGAGCACTACAACAGAGCCACTACGGGCGACCGCCCGAAcgga > 2:343928/1‑142 (MQ=2) cACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaa < 1:58500/143‑1 (MQ=32) gACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGCCGACGGCGCGAAGGccgagcgactcg > 1:639505/1‑139 (MQ=255) aCCCACCAATGTACAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagcga < 1:298690/143‑1 (MQ=17) ccaccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACTTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACCACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGt > 1:325284/1‑143 (MQ=17) ccaccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGGAGGGCGAGCGAAGCGAGt > 1:821500/1‑143 (MQ=17) gTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGACGGGCGAGCGAAGCGGGTCATCCTGc > 1:173052/1‑143 (MQ=17) tAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCATGCa > 1:627405/1‑143 (MQ=17) aaaaaaGCGCCTTTAGGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCt > 2:292683/1‑99 (MQ=11) aaaaaaGCGCCTTTAGGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCt > 2:755371/1‑99 (MQ=11) aaaaaaGCGCCTTTAGGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCt < 1:292683/99‑1 (MQ=11) aaaaaaGCGCCTTTAGGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCt < 1:755371/99‑1 (MQ=11) aaaaaGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGACGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACg > 2:840303/1‑143 (MQ=17) aaaaGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGACTCGCACCTGCAGCAGGGCTGAGTTGAGCGCAGCGAAACCACGGAGCCGCTCGCGGCGACGGCACGAAGGGAGCACGAAGCAAGTTGACCTGCACGa > 2:35424/1‑143 (MQ=11) aaGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGAcc > 1:746302/1‑143 (MQ=32) tAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATg < 1:651451/143‑1 (MQ=32) | GCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATG > NC_000913/781531‑781811 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |