Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	pseudogene (151/758 nt)	glpR ←	DNA‑binding transcriptional repressor GlpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	31.1 / NA	13	pseudogene (151/758 nt)	glpR	DNA‑binding transcriptional repressor GlpR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (9/4);  total (9/4)

CGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGAT  >  NC_000913/3560315‑3560575                                                                                                                                              |                                                                                                                         cGATAGTCATCGTCGGGCGTTTGGGGATTTGCTCCGTGATTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcgc                                                                                                                         <  2:14843/143‑3 (MQ=255)  gagaaaCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGACCTGGAAGGCAGCGCCGCACCGCCATGATGGcgca                                                                                                                        >  2:562481/4‑140 (MQ=255)  gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGACCTTGCGATCGTGCCACGGCGTGTTAACCGAAGTGAAAGGCAGCGCCGCACCGCCATGATGGcgca                                                                                                                        >  2:66677/1‑140 (MQ=255)                        ggggTTTGCTCCGCCACGTTGCGGGCGATGCGCTCGTTTTCTTGGGTCTGGGTGGCCTTGCGATCGCGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAg                                                                                                  <  1:70192/143‑1 (MQ=255)                                   cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGCTCGTGCCACGGCGTGTTAACCGACCTGGGAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTCTGCTCCGCCAGCTCATTGAGGt                                                                                       >  1:405595/1‑143 (MQ=255)                                             cGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt                                                                             >  1:169498/1‑143 (MQ=255)                                                    tGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc                                                                      >  1:520656/1‑143 (MQ=255)                                                      cgcCCTTTTTCTTCGGTCTGGGTGGCCTTGAGATCGCGCCACCTCGTGTTAACCGGACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg                                                                    <  1:243839/143‑1 (MQ=255)                                                                 ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGGGGTCGCGGCGAATAGTCTGCGGGCTGACGgaga                                                         >  2:426019/1‑143 (MQ=255)                                                                     gTCTGGATGGTCTTGCGATCGTGCCACGGCGTGGTAACAGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg                                                     <  1:193973/143‑1 (MQ=255)                                                                                 ttGCGATCGTGTCACGGCGTGTTAACCGAACTGGGAGGCAGCGCCGCCCCGCCATGATGGCGGCGGATCAGGCTCTGCTCCGCCAGCTCACTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGACACGCTCTACCAGcgc                                         >  2:38989/1‑141 (MQ=255)                                                                                     gATCGTGCCACGGCGTGTTAACCGAACTGGAAAGCAGCGCCGCAGCGAGATGATGGCGCAGGATGAGGTTTTGCTCCGCCAGCTCCTTGCCGAAGCCGCGAATAGTGTGCGGGCTGACGGGCCCATGCACTACCAgactgtgc                                     >  2:231833/1‑136 (MQ=255)                                                                                          tGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGACGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATCGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTCCGGTGCt                                >  2:357436/1‑143 (MQ=255)                                                                                                               cggcaaGGCAGTGCAGCACCGCCTGCATGGCGCAGGATCACGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc           <  2:434177/139‑1 (MQ=255)                                                                                                                        aGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTCCTGACATAACCCTGCTGTTTAACCAGTTCGat  >  1:197959/1‑143 (MQ=255)                                                                                                                        aGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTATAGCAGCTCTTGGGTACTGACATAACCCTGCTGTTTAACCAGTTCGat  >  1:62695/1‑143 (MQ=255)                                                                                                                                              |                                                                                                                         CGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGAT  >  NC_000913/3560315‑3560575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑