Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,815,859	Δ82 bp		[rph]	[rph]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3815859	3815940	82	48 [0]	[0] 48	[rph]	[rph]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3815858	0 (0.000)	45 (1.490)	42/264	0.0	100%	intergenic (‑90/+5)	pyrE/rph	orotate phosphoribosyltransferase/truncated RNase PH
	?	NC_000913	3815941 =	0 (0.000)					coding (609/687 nt)	rph	truncated RNase PH

CCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT  >  NC_000913/3815720‑3815997                                                                                                                                           |                                                                                                                                            ccTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTATTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtc                                                                                                                                         <  2:576472‑M1/143‑5 (MQ=255)   tgcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtccc                                                                                                                                       <  2:129401‑M1/143‑7 (MQ=255)        gctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTACAAATCAGTTTGCCAGCGCCGCCCTCTGCcgtccccagcc                                                                                                                                  >  2:450886‑M1/1‑132 (MQ=255)        gctAAGCGCAAATCCAATAACCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGCCTCCTAAGTTGCCTGTAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgca                                                                                                                                  <  1:558027‑M1/143‑12 (MQ=255)         ctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAGAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGCTTGCCAGCGCCGCCTCCTACcgtcccctgcac                                                                                                                                 >  1:435296‑M1/1‑131 (MQ=255)           aaGCGCAAATTCAACAACCTGGCGCTGATATGGTTTCACGCCCTCGCTCCTCATCTTACTTTTCTACCGACAAAAAAAAGGCGACTCATCAGTCGCCTCAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcactt                                                                                                                               <  1:141005‑M1/143‑15 (MQ=255)            aGCGCAAACTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttc                                                                                                                              <  1:83645‑M1/143‑16 (MQ=255)              cgcACAGTCAATAAACTGGCGCTGAGATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaa                                                                                                                            <  1:161307‑M1/143‑18 (MQ=255)               gcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaac                                                                                                                           >  2:327850‑M1/1‑125 (MQ=255)                   aTTCAACAAACTGGCGCTGATATGGTTTCATTCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatg                                                                                                                       <  2:174683‑M1/143‑23 (MQ=255)                     tCAATCGACTGGCGCTGATGTGGTTTCATGCCTTCGCTCCTCATCTTACTTCTCTACAGACAAAAAAAAGGCGACTCCTCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcg                                                                                                                     <  2:285978‑M1/143‑25 (MQ=255)                     tCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcactgatgcg                                                                                                                     >  1:38618‑M1/1‑119 (MQ=255)                              tGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttc                                                                                                            >  1:404269‑M1/1‑110 (MQ=255)                               ggCGCTGATATGGTTTCATGCCTACGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGACTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcg                                                                                                           <  2:503291‑M1/143‑35 (MQ=255)                                           gTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcacgacg                                                                                               >  2:52029‑M1/1‑97 (MQ=255)                                           gTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTGTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacg                                                                                               <  2:492259‑M1/143‑47 (MQ=255)                                                aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcat                                                                                          <  1:196911‑M1/143‑52 (MQ=255)                                                    cTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgaccgtcttcggtaatcactacgttcatgtcg                                                                                      >  1:530425‑M1/1‑88 (MQ=255)                                                     ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttccatgatacgcccgtcttcggtcatcactacgtccctgtcgg                                                                                     >  1:454729‑M1/1‑87 (MQ=255)                                                     ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcgg                                                                                     >  2:384326‑M1/1‑87 (MQ=255)                                                         ctcctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttccatgatgcccccgtcttcggtcatcactacgatcctgtccgtctc                                                                                 >  1:369799‑M1/1‑83 (MQ=255)                                                            ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgc                                                                              <  1:395189‑M1/143‑64 (MQ=255)                                                                tCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggca                                                                          >  2:557230‑M1/1‑76 (MQ=255)                                                                 cTTAGTTTTCTACATACAAAAACAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcag                                                                         <  2:585415‑M1/143‑69 (MQ=255)                                                                 cTTACTTTTCTACAGACAAAAAAAAGGCTACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcag                                                                         <  2:519499‑M1/143‑69 (MQ=255)                                                                  ttACTCTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCGGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcaga                                                                        <  2:472708‑M1/143‑70 (MQ=255)                                                                      ttttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtct                                                                    >  2:104289‑M1/1‑70 (MQ=255)                                                                        ttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgacgctctctgcgccagaggcgtc                                                                  >  2:244044‑M1/1‑68 (MQ=255)                                                                                aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcgctacgttcatgtcggtctctgcggcagaggcttcaacgtatc                                                          >  1:132406‑M1/1‑60 (MQ=255)                                                                                    aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtcttccag                                                      >  1:100802‑M1/1‑56 (MQ=255)                                                                                     aaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgccttccagc                                                     >  2:270095‑M1/1‑55 (MQ=255)                                                                                      aaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagat                                                    <  1:543600‑M1/143‑90 (MQ=255)                                                                                        aaGGCGACTGATGAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcggagtcttcaacgtattcccgatcg                                                  >  1:542148‑M1/1‑52 (MQ=255)                                                                                         aGGCGACTAATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacgtcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtgtctgcgccagaggcgtcaacgtattcgagaaccc                                                 >  2:63617‑M1/1‑51 (MQ=255)                                                                                                  atcaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgcaaaccgctt                                        >  1:312622‑M1/1‑42 (MQ=255)                                                                                                   tcaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatccctacgttcatgtcggtctctgcggcagagtcttcaacgtcttccagatcgcacaccgctcc                                       >  1:138994‑M1/1‑41 (MQ=255)                                                                                                          ccTTAAAAATCAGTGTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgcaaaccgcttcgccgttc                                <  2:518624‑M1/143‑110 (MQ=255)                                                                                                            ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgcaaaccgcccagccgttcac                              >  1:361266‑M1/1‑32 (MQ=255)                                                                                                              aaaaaTCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacggattccagatcgcaaaccgcttcgccgctcacaa                            >  2:213299‑M1/1‑30 (MQ=255)                                                                                                                   tCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgcaaaccgcttcgccgttcacaattccg                       >  1:289880‑M1/1‑25 (MQ=255)                                                                                                                   tCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacggagtccggatcgcaaaccccctcgccgttcacaattccg                       >  2:531858‑M1/1‑25 (MQ=255)                                                                                                                    ccgttGGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgcaaaccgcttcgccgttcacaattccga                      <  1:185246‑M1/141‑120 (MQ=255)                                                                                                                       tttGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgcacaccgcttcgccgttcgcaattccgacag                   >  2:339339‑M1/1‑21 (MQ=255)                                                                                                                            ccgccccgccttctgCcgtcccccgcacttcaatgatgcgcccgtcttcggtcatcaccacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgcaaaccgcttcgccgttcacaattccgacagaaact              <  1:468819‑M1/138‑128 (MQ=255)                                                                                                                               cgccgcCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgcaaaccgcttcgccgttcacaattccgacagaaactgcg           >  1:443524‑M1/1‑13 (MQ=255)                                                                                                                                      ttCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgccaaccgcttcgccgttcacaattccgccagaaactgcggctacca    >  1:376332‑M1/1‑6 (MQ=255)                                                                                                                                      ttCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgcaaaccgcttcgccgttcacaatcccgacagacactgcggctaccc    >  2:417219‑M1/1‑6 (MQ=255)                                                                                                                                        cTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctggggcagagtcttcaacgtattccagatcgcaaaccgcttcgccgttcacaattccgacagaaactgcggctaccatc  <  2:38618‑M1/143‑140 (MQ=255)                                                                                                                                           |                                                                                                                                            CCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT  >  NC_000913/3815720‑3815997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑