| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 76,805 | T→A | E165D (GAA→GAT) | sgrR ← | DNA‑binding transcriptional dual regulator SgrR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 76,805 | 0 | T | A | 95.7% | 63.1 / NA | 23 | E165D (GAA→GAT) | sgrR | DNA‑binding transcriptional dual regulator SgrR |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (8/14); minor base G (1/0); total (9/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCGTATTGATTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACG > NC_000913/76667‑76918 | cAGCGTATTGATTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCa < 1:419137/143‑1 (MQ=255) aGCGTATTGACTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAg < 2:582099/143‑1 (MQ=255) ttCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCAttt < 2:364441/143‑1 (MQ=255) gTTTTAAAGAGGCGAGCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTcc < 1:237122/143‑1 (MQ=255) tgaaGAGGCGATCCCATCGGCCAGTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCAt < 2:454555/141‑1 (MQ=255) agagGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAACAGAAACGCGAGTGAAGCGGTGAACTTTGCTGCCAGCGGTGGGCGATGTCTGCATCCAGTGCCACATTTTCCTcctcta > 2:162795/1‑138 (MQ=255) gagGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGGGGAGGGCGATGTCTGCATCCAGTTCCCCATTTGCATCATGTAt > 2:19742/1‑143 (MQ=255) aTCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCCGTGAAGCGGTGAAATTTACTGCCAGTGGTGGGCGATGTCTGCGTCCAGTTCCCCGTCTTCCTCATCTGTGCGCGt > 1:466704/1‑143 (MQ=255) aTCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGACACGCCAGTGAAGCGGCGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGGTCCCCCTTTTCCTCCTTTGTGCGCGCGAGCg > 2:222459/1‑143 (MQ=255) aTCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTGCTTTATGCGCGTTcgcg > 2:321852/1‑143 (MQ=255) ccgccATTTCCACTTCACGACCATGGTGGAAATGGTCTCCTGGACGCAAAAGGAAACGCCAGTGAAGCGGTGAAAGTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGaa < 2:59915/140‑1 (MQ=255) cGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGaa < 2:168470/143‑1 (MQ=255) tCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGGGGTGGGCGATGTGTGCATCCAGTTGCCCATTTTCCTCCTCTAGGAGCGTTAGCGAACt > 2:200185/1‑143 (MQ=255) aTTGCCAGGTCTCGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGaa < 1:222065/143‑1 (MQ=255) ttatgACCATGGTGAAATTCAATTCCTCTATGCAAAAAGAGCCGCCAGTGAAGCTGTGAAATGTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCg < 1:293088/139‑1 (MQ=255) aaaaCGGACTGCTGGACGGAAAGAGCAACGCGAGTGCAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGCCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGttt < 2:272024/143‑1 (MQ=255) gACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAAc > 1:81207/1‑143 (MQ=255) tcTCCTGGACGCAAACAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACg < 1:232834/142‑1 (MQ=255) aactcAAAATGGAACGCCAGTGATGCTGTTAATTGTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCTTTTTCCTCCTTTAGGCGCGTTAGCGAACTGAAGATTTGCCGTGTGATATGTGTTTCGGAACGGCGCAAt < 2:558092/139‑1 (MQ=255) aaaaaGAAACGCCAGTGAAGCGTGTATATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCt < 2:3020/143‑1 (MQ=255) cAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAg > 2:348981/1‑143 (MQ=255) aGCTGTGAAATTTGCTGCCAGGGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACg < 1:157953/143‑1 (MQ=255) aTTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTCGCTATCTCTAGTTTTGCCGGGCGATATGGGTTTCGTTACAGCGCAATGCCGTGCCAGATAGCAGGTTAAGCCACGGAca > 1:40975/1‑142 (MQ=255) | CAGCGTATTGATTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACG > NC_000913/76667‑76918 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |