| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,416,622 | C→G | Q626E (CAG→GAG) | pta → | phosphate acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,416,622 | 0 | C | G | 92.0% | 67.9 / ‑6.2 | 25 | Q626E (CAG→GAG) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base G (14/9); total (15/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCG > NC_000913/2416487‑2416760 | ggTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTacga < 1:447242/143‑1 (MQ=255) tATCGAACCGCGCGGTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTacgacg < 1:46483/143‑1 (MQ=255) cggaCCGCGCGTTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGCAGCCACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTg < 1:526633/140‑1 (MQ=255) gcgcgGGGCTATGCGCTCCTACACCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTaa < 1:148240/143‑1 (MQ=255) cgcgTGGCTATGCTCTCCTACTCCACCGGGACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAAt < 1:524473/143‑1 (MQ=255) gCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCGACGGTAATGGCTGa > 2:213550/1‑143 (MQ=255) tGCTCTCCTACTCCACCGGTGCTTCTGGTGCAGGTAGCGACGTAGAAACAGCTCGCGAAGCGACTCGTCTGGCGCAGGAACAACGTCCTGACCCGACGCTCGACGGGCCGCTAGCGTGCCACGCTCCGGCGATGGCTGCCgcc > 2:576128/1‑141 (MQ=255) ggTAGCGACGTAGAACAGGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGc < 2:165482/143‑1 (MQ=255) tAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGccg > 2:126271/1‑141 (MQ=255) gACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTcg > 1:199500/1‑143 (MQ=255) gTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGCCGTTGCGCCATCCACCGCGCCGCCCTCTCCGGTTGCCGGTCgcgc > 1:66990/1‑143 (MQ=255) gAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCATGACCTGATGATAGAAGGTCCGATGGAGTACGACGCTGCGGTCATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTAc > 1:545013/1‑143 (MQ=255) aaaaaGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGAACTGATACTATTCGGTCCGCACCACAACAATGTTGTGTTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGGCGCGCTAcc > 1:135394/1‑143 (MQ=255) ttCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGCTTGCAGGTCGCGCTCCCGTGTTc > 2:247443/1‑143 (MQ=255) cgAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCt > 1:316899/1‑143 (MQ=255) cgAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCt > 1:64587/1‑143 (MQ=255) aaGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCCCCGGCTGCCGGTCTCTCTACCGTGTTCATCTTc > 2:228901/1‑143 (MQ=255) aaCTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCgg > 2:247625/1‑143 (MQ=255) cGTCTGGCGCAGGAACAGCGTCCTGCCCTGATGTTCGACGGTCCGCTGGAGTACGACGCTGCGGTAGTGGCTGACGTGGCGAAATCCAACGCGCCGTACTCTCCGGTTGCGGGTCGCGCTACCGTGTTCATCTTCCCGGATCt < 2:12646/143‑1 (MQ=255) catgAAATACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCgg < 2:303813/140‑1 (MQ=255) gAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGCACACCGGTaa > 1:384922/1‑143 (MQ=255) aaaaCGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTCCCGTGTTCATCTTCCCGGATCTGAACACCGGTcaca > 1:32289/1‑143 (MQ=255) tcggcctgagctgaTGATCGACGGTCCGCTGGAGTACGACGCTGCGGTACTGGGTGTCGTTGCGAACTCCAGAGCGCCGAACTCTCCGGTTGCACATCGCGTTTAAGTGTTCTTCTTCCCGGATCTGAACACCGGTAAcacca < 2:545013/139‑1 (MQ=255) tgatgaTCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGCACACCGGTACCACCACCTCCAAcgcg > 1:549642/1‑143 (MQ=255) gCTTCATTACGACGCTGCGGTAATGGCGGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCg < 1:578577/143‑1 (MQ=255) | GGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCG > NC_000913/2416487‑2416760 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |