| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,041,398 | T→G | L29R (CTT→CGT) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,398 | 0 | T | G | 95.0% | 51.2 / ‑6.7 | 20 | L29R (CTT→CGT) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (9/10); minor base A (1/0); total (10/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGT > NC_000913/3041265‑3041534 | ttttACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCAt > 2:280189/1‑143 (MQ=255) aCCGTTAGTCATTAGTTTGCGGTTAAGCAACTCAGGACTGTATTCTCGCTTGTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACt < 2:536461/143‑1 (MQ=255) cATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTAATATGGCTTTTACACCTCTTCCTCCCCGTCCGCCTACTGCTTCTGCCCGTTTACCACTGACGCTGACCACGCTCGATGACCGCCCGCACGCCACCactactcg > 2:417504/1‑141 (MQ=255) ttAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGTCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCTTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGgcg < 1:177969/143‑1 (MQ=255) ttAGTCATTAAGACGCCGTTAAGCAACTCAGGACTCTCTTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCCACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGgcg < 1:479135/143‑1 (MQ=255) tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGa < 1:325758/143‑1 (MQ=255) tAATGCGCCGTTAGGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGCCGCTTGATGACTGGGCGCGTGCCACCATGAATGGCGCGGACAGc > 2:13997/1‑143 (MQ=255) aaTACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCg < 2:400396/143‑1 (MQ=255) aaTACGCCGTGACGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCg < 2:247058/143‑1 (MQ=255) cGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGaaaa > 2:297410/1‑143 (MQ=255) aGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCCCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAGTCTATGc > 2:342278/1‑143 (MQ=255) cAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAg < 1:280189/143‑1 (MQ=255) aCTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGCCGCTTGATGACTGGGCGCGTGCCGCCATTACTGGCGCGGACCGCGAAAACTATGTGCAgcg > 2:587931/1‑141 (MQ=255) cTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGCTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCGAGGTCAGGTGACCGCAGACGTCAGCCAGATg > 2:104391/1‑143 (MQ=255) ccGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATc < 1:530118/143‑1 (MQ=255) gCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCa > 1:398894/1‑143 (MQ=255) cGTTTACCGCTGACGCTGGTTACGCTTGATGACTGGGCGCGTGCCACCCTTCCTGGCGCGGCCAGCGAAAAATATCTGCAGGGCCAGGTGCCAGCCGATGTGAGCCAGATGGCACAAGATCAGCCCCTGCGCGCCGacccgtg > 2:388406/1‑139 (MQ=255) cgaacgcggacgctgcTGACGCTTGATGACTGGGCGCGTGCCACCATTACGGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGa < 1:154049/139‑1 (MQ=255) gcgtgatgaCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGa < 1:414682/140‑1 (MQ=255) gggCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTCCGt > 1:343934/1‑143 (MQ=255) | TTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGT > NC_000913/3041265‑3041534 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |