| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 87,481 | C→A | T42K (ACA→AAA) | ilvH → | acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 87,481 | 0 | C | A | 95.8% | 61.0 / NA | 25 | T42K (ACA→AAA) | ilvH | acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (8/15); total (8/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCGGGAAATCATGCT > NC_000913/87341‑87619 | cGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACAGTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAAt < 1:357914/143‑1 (MQ=255) agagaACCTGATTATGCGGCGGATATGACCAGTCTTACTCGAAAATGAATCGCGCGCGTTATCCCGCGTGACTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGGTGCGCCCACCGACGATCCGAAATTAt < 2:498346/143‑1 (MQ=255) gagaACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATc < 1:325531/143‑1 (MQ=255) cTGATCATGCGCCGGATCTTATCAGGCTTACTCGAAAATGAATCAGGCGCCTTCTCCCGCGTGCTTGGCCTTTCTCCCCACCCTCGCTACAACATCGAACCCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTAt < 1:275750/143‑1 (MQ=255) attatCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTCTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTgg < 2:115988/143‑1 (MQ=255) gggcgcGAAAATGAAGCAGGCGCGTTATCCCGCGTGAGTGGCCTGTTGCCCCAGCGCGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGaaaa < 1:154826/138‑1 (MQ=255) tCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGACCAAGTa > 2:104320/1‑143 (MQ=255) aaaaTGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACtt > 1:230949/1‑143 (MQ=255) aTCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCGTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACCTGAGCAGa > 2:106044/1‑143 (MQ=255) cgcggCTCGTCATCCCGCGTACCTGGCCTTTTTTCCCAGCGTAGCTACAACATTGCCACCCTGACCGTCGCGCCACCCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGAt < 1:443692/140‑1 (MQ=255) gTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGGCCATCCAGACCGTGGGCGATGAAAAAGGACGTGAGCAGAGCGAAAAgg > 1:571357/1‑142 (MQ=255) gTTATCACGCGTGACGGGCCTTTTGTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATTCGAAATTATCGCGGGTGACCATACAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc < 2:517802/143‑1 (MQ=255) ttttttCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGAt > 1:494023/1‑143 (MQ=255) cGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGAGCCGTATTTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGcgcgc > 1:558309/1‑142 (MQ=255) ttgcgacaataTTGAAAGCTTGAGCTTTGCGCCAACCGACGATCCCAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAACCTGGTCGATGTCTTGCGCgtga < 2:84150/138‑1 (MQ=255) tGGCTACAACATTGAGAGCCTGAGCGTTGCGGCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCgtga < 1:262956/143‑1 (MQ=255) acaTTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTg < 2:230949/143‑1 (MQ=255) caTTGAAAGCCTGACCGTTGCGCCAACCGGCGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAGACTGGTCGATGTCTTGCGCGTGAGTGAGTTgg < 2:268760/143‑1 (MQ=255) ttGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTgggg > 1:557484/1‑143 (MQ=255) gAAAGCCTGCCCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCa < 2:282464/143‑1 (MQ=255) gacgCCGGACCGTTGGGCCAACCGGCGATCCGAAATTATCGCGTTTGACCGTCCAGTCCGTGGGCGATGAAAAAGTACTTGAGCAGCTTGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAg < 2:294105/140‑1 (MQ=255) aGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAggg > 1:224146/1‑143 (MQ=255) ccAACCGACGAGCAGACATGTTCCCTGCTCACCATCCAGACCGGGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAGTACACAAACTGGGCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCg < 2:379568/143‑1 (MQ=255) cAACCGACGAGCCGATAGTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCgg < 2:227853/143‑1 (MQ=255) ccGAAAGTATCGCGTAGGACCATCCAGACCGGGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGCGATTGGGCAGGGCGCGCATGTTGAGCGGGAAATCATGCt < 1:402426/143‑1 (MQ=255) | CGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCGGGAAATCATGCT > NC_000913/87341‑87619 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |