Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,220	C→T	H366H (CAC→CAT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,220	0	C	T	94.4%	36.6 / ‑5.4	18	H366H (CAC→CAT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (8/9);  minor base G (0/1);  total (8/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAT  >  NC_000913/1196087‑1196295                                                                                                                                      |                                                                            tcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAGGGGTggg                                                                    >  1:176305/1‑143 (MQ=255)  cgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGGCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGa                                                                   <  2:179184/143‑1 (MQ=255)         ggTGCAAACAGCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGc                                                            <  1:393120/143‑1 (MQ=255)           tGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGCGGTGAGGCTGCGCCATATGGGTTGGACTGAAGCgg                                                          >  1:132046/1‑143 (MQ=255)                 cagcGGTGACGAAGGCGCCCTGGTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcc                                                    <  2:73548/140‑2 (MQ=39)                 cagcGGGGACGCATGCGCCCTGTTTGGAGCCGCCCACGGTACTGCGCCGAAATAGGCCGGCCAGGACAAAGTCTTTCCCGGCTCTATTATGCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcc                                                    <  2:283723/140‑2 (MQ=25)                     ggTGACGAATGCGCCCTGTTTGAAGGCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGAGGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcctgat                                                <  2:46254/143‑6 (MQ=38)                            aaggcgcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTGTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                         <  1:542157/140‑1 (MQ=38)                             gggcgcgcTGTTTGAGGCCACCCACGGTACTGCGCCGAAATATGCCGGGCAGGACAAAGTAAATCCTGGCTCTCTTAAGCTCTTCGCGGAGATGATGCTGCGGCAGATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg                                        <  1:24948/141‑1 (MQ=25)                                           aaGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCa                          >  1:529460/1‑143 (MQ=25)                                           aaGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGATGACCTGATTGTTCAAGGTATGGAAggagaa                          >  2:184975/1‑139 (MQ=37)                                                 cccacGGTACTGCGCCGAAATATGCCGATCAGGAGAAGGTAGATACGGGCTCGATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTGGGACTGAGGCGGCTGACCTGATTGTTGAAGGTATGGGAGGGgcaagcaat                    >  1:498369/1‑142 (MQ=255)                                                  ccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatg                   >  2:299150/1‑141 (MQ=255)                                                   cacGGTAGTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc                  <  1:246306/143‑1 (MQ=21)                                                           tGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGCTTGTTGAAGGTGTGGAAGGCGCAATCAGTGCCAAGACCg          >  2:532604/1‑143 (MQ=18)                                                             cgcCGAAATCTGCCGGTCACGACAAAGTAAGTCCTGGCTCGCTTGTTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTTGACTGCAGCGGCTCACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTa        <  2:578208/143‑1 (MQ=17)                                                                cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAAACCGTAAct     >  2:155250/1‑142 (MQ=18)                                                                   catatGCCTGGCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGACGACGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTTATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt  <  1:332473/142‑1 (MQ=14)                                                                                                                                      |                                                                            TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAT  >  NC_000913/1196087‑1196295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑