Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,247	A→G	L375M (TTA→CTG)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,247	0	A	G	90.9%	14.9 / NA	11	L375M (TTA→CTG)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (6/4);  minor base T (0/1);  total (6/5)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

AATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAT  >  NC_000913/1196114‑1196295                                                                                                                                      |                                                 aaggcgcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTGTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                         <  1:542157/140‑1 (MQ=38)  gggcgcgcTGTTTGAGGCCACCCACGGTACTGCGCCGAAATATGCCGGGCAGGACAAAGTAAATCCTGGCTCTCTTAAGCTCTTCGCGGAGATGATGCTGCGGCAGATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg                                        <  1:24948/141‑1 (MQ=25)                aaGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCa                          >  1:529460/1‑143 (MQ=25)                aaGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGATGACCTGATTGTTCAAGGTATGGAAggagaa                          >  2:184975/1‑139 (MQ=37)                      cccacGGTACTGCGCCGAAATATGCCGATCAGGAGAAGGTAGATACGGGCTCGATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTGGGACTGAGGCGGCTGACCTGATTGTTGAAGGTATGGGAGGGgcaagcaat                    >  1:498369/1‑142 (MQ=255)                       ccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatg                   >  2:299150/1‑141 (MQ=255)                        cacGGTAGTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc                  <  1:246306/143‑1 (MQ=21)                                tGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGCTTGTTGAAGGTGTGGAAGGCGCAATCAGTGCCAAGACCg          >  2:532604/1‑143 (MQ=18)                                  cgcCGAAATCTGCCGGTCACGACAAAGTAAGTCCTGGCTCGCTTGTTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTTGACTGCAGCGGCTCACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTa        <  2:578208/143‑1 (MQ=17)                                     cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAAACCGTAAct     >  2:155250/1‑142 (MQ=18)                                        catatGCCTGGCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGACGACGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTTATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt  <  1:332473/142‑1 (MQ=14)                                                                                                                                      |                                                 AATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAT  >  NC_000913/1196114‑1196295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑