New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 417180340 (1.510)5 (0.190) 5/260 NT 10.3% intergenic (+47/‑254) rrfB/murB 5S ribosomal RNA/UDP‑N‑acetylenolpyruvoylglucosamine reductase
?NC_000913 = 4213162 48 (1.840)intergenic (+3/‑72) rrfE/yjaA 5S ribosomal RNA/stress response protein

GGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTG  >  NC_000913/4213021‑4213300
                                                                                                                                             |                                                                                                                                          
ggTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAa                                                                                                                                           >  1:340434‑M2/1‑142 (MQ=255)
 ctcTGAGAAAACAGAATTCGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAat                                                                                                                                          >  1:372777‑M2/2‑141 (MQ=255)
  tCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCAGCTGGCAGCGGGTGGGCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAAtt                                                                                                                                         >  1:490506/1‑143 (MQ=11)
  tCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCAACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAata                                                                                                                                         <  1:142876‑M2/143‑4 (MQ=255)
      aTAAAACAGAATTGTCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGaa                                                                                                                                     <  2:321643/143‑1 (MQ=18)
          aaCAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTCGGGAACTGCCAGGCATCAAatacaacgcaa                                                                                                                                 <  2:485142‑M2/143‑12 (MQ=255)
             aGAATTTCCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAAcccc                                                                                                                              <  1:174987/143‑1 (MQ=255)
              gattttGCCGGGCGGCAGTAGCGCGGTGGTCCCACCTGGCCCCAGGCCGAACCCAGAAGTGAAACGCCGTAGGGCCGATGGTAGTGCGGGGGCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCg                                                                                                                             <  2:153818/140‑1 (MQ=14)
              caagttGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCg                                                                                                                             <  2:40258/139‑1 (MQ=255)
                 cttGCCTCGCGGCAGTAGCGCGGCGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTc                                                                                                                          <  1:34320/142‑1 (MQ=255)
                    gCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGGAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGCTCCAt                                                                                                                       >  1:110443/1‑143 (MQ=255)
                      cTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGGAACGCCGTAGCGCCGGTGGTAGTGTGGGGTCTCCCCATGCGAGAGTCGGGAACTGCCAGGCATCAAAGTAGAAAGACCGCGGTCTATaa                                                                                                                     >  2:298123/1‑143 (MQ=11)
                      cTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGCGGGGTCGCCCCATGCGAGAGTAGGGAAGTGCCAGGCGTCCAATTAGACAAAGCCCGGTCCAGaa                                                                                                                     >  2:163403/1‑143 (MQ=14)
                         gcggcAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGGAGTGAAACGCCGTCGCGCCGATGGCAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGCCCATAAGGc                                                                                                                  >  2:97208/1‑143 (MQ=255)
                              aGTAGCGCGGTGGCCCCACCTGCCCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCACATGCGAGAGTAGGGAACTGCCAGACATCAAataaaacaaaaggcccagtcttccgactgag                                                                                                             >  2:61140‑M2/1‑112 (MQ=255)
                                 aGCGCGGTGGGCGCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaaggcccagtctttcgactgagcct                                                                                                          >  2:303519‑M2/1‑109 (MQ=255)
                                   cgcgGTGGTCCCACCTGACCCCATGCCGACCTCAGACGTGAAACGCCGTATCGCCGATGGTAGTGTCGGGTCTCCCCATGCGAGAGAAGGGAACTGCCAGCCATCAAATTAGAAAAACCCCGGTCCATAAGGCCgggggtatt                                                                                                        >  2:159013/1‑138 (MQ=17)
                                   cgcgGTGGGCCGACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTGGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaaggcccagtctttcgactgagccttt                                                                                                        >  2:166768‑M2/1‑107 (MQ=255)
                                    gggttggtGCCACCTGAGCCCTTGCGGAACTGCGAAGTGAAACGGCGTAGCGTCGATGGTAGTGTGGGGGCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCACATTAGAAAAACCCCGGTCCATAAGGCCGGGGtttttt                                                                                                       <  1:215456/139‑1 (MQ=18)
                                     cgGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTg                                                                                                      <  1:83548/143‑1 (MQ=255)
                                      ggtggtCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGACGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGc                                                                                                     <  2:328094/143‑1 (MQ=255)
                                       gtgggCCCACCTGACCCCATGCCGAACTCAGGAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCa                                                                                                    <  1:12895/143‑1 (MQ=255)
                                           tCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAACAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATc                                                                                                >  2:267052/1‑143 (MQ=255)
                                            gcgacctGGCCCCAGGCCGGACTCAGGAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCAAGAGTAGGGAACTGCCAGACATCAAataaaacaaaaggcccagtcttccgactgagccttttgttttatt                                                                                               >  2:136154‑M2/4‑98 (MQ=255)
                                            cccACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGCAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCACAAGGCCGGGGGTTGTTCCATACCa                                                                                               >  2:484631/1‑143 (MQ=17)
                                               aCCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGCTGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGCAACTGCCAGGCATCAAATTAGAAACACCCCGGTCCATAAGGCCGGGGTCTTTTGCATATCAatt                                                                                            >  2:68974/1‑143 (MQ=255)
                                                ccTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAAGTAGAAAAACCCCGGTCCATAAGGCCGGGGGGTTTTGCATGTCAatta                                                                                           >  1:10998/1‑143 (MQ=255)
                                                   gACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTAttt                                                                                        <  1:269592/143‑1 (MQ=255)
                                                    aCCGCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTg                                                                                       <  2:212930/143‑1 (MQ=255)
                                                        cATGCCGCACTCAGAAGTGAACCGCCGTAGCGCCGGTGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAATCCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCatg                                                                                   <  1:497748/143‑1 (MQ=255)
                                                         aTGCCGAACGCAGAAGTGAAACGCCGCAGCGCCGATGGGAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCatga                                                                                  <  1:252223/143‑1 (MQ=255)
                                                                       aGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGACAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCa                                                                    <  1:110216/143‑1 (MQ=255)
                                                                       aGTGAAACGCCGGAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCa                                                                    <  2:65381/143‑1 (MQ=255)
                                                                        gTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCAt                                                                   >  1:232699/1‑143 (MQ=255)
                                                                            aaCGCCGTAGCGGCGAGGGTAGTGTGGGGTCTCCCCGTGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCa                                                               <  1:71629/143‑1 (MQ=255)
                                                                                ccGTAGCGCCGATGGTAGTGTTGGGCCCCCCCATGCCAGAGTAGGGAACTGCCAGGCATCAAATTAGAAACACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTc                                                           <  1:172008/143‑1 (MQ=37)
                                                                                   tAGCGGCGTGGGTAGTGTGGGGGCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGt                                                        <  1:232114/143‑1 (MQ=255)
                                                                                     gcgcCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGtat                                                      >  1:231148/1‑143 (MQ=255)
                                                                                                    tgGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTcgtcgt                                       >  2:56463/1‑143 (MQ=255)
                                                                                                            ccccATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTATGCATTGGGATGGTTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAAt                               <  1:325136/143‑1 (MQ=38)
                                                                                                             cccATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAGACCGCTGTCCATAAGGACGGGGGTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTGATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAAtt                              >  2:423159/1‑143 (MQ=39)
                                                                                                             cccATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAAtt                              >  1:152822/1‑143 (MQ=255)
                                                                                                              ccATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGACGGGAGTCTCATGTCAGTCCTGTATATCCAAATTCGCCGTAATCACATTa                             >  1:191771/1‑143 (MQ=39)
                                                                                                                  gCGAGAGGAGGGACCTGCCAGGCATCAGATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGt                         <  1:508780/143‑1 (MQ=255)
                                                                                                                    gagaGTAGGGAACTGCCAGGCATCGAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCTTCGTAATCAAATTACTGTTc                       <  2:158394/143‑1 (MQ=255)
                                                                                                                    gagaGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTc                       <  1:314479/143‑1 (MQ=255)
                                                                                                                        gtggggAACAGGCAGGCATCAAATTAGAAAAACCCCTGTCCATAAGGCCGGGGTTTTTTGCATATGAGTTGTTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGa                   <  2:166756/140‑1 (MQ=255)
                                                                                                                             ggaCTGCGCGGCATCAAGTTAGAGAAGCCCCGGGCCATAAGGCCGGGGTTTTTTGCATATCAATGATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTg              <  2:268721/141‑1 (MQ=255)
                                                                                                                               gcTGCCAGGTATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGaa            <  2:118514/142‑1 (MQ=255)
                                                                                                                               aCTGCCAGGCATCTAATTGGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGaa            <  2:160720/143‑1 (MQ=255)
                                                                                                                               aCTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGaa            <  1:36017/143‑1 (MQ=255)
                                                                                                                                gtGCTAGGCATCAAATTAGAAAAACCTCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGGCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTTGCGATCTTGaaa           <  2:264287/142‑1 (MQ=255)
                                                                                                                                cTGGCAGGCATCAACTTAGAAAAACCGCGGTCCATAAGGGCGGGGTTGTGTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGGATATCCAAATGCGTCGTAATCAAATTACTGTTCGCGATCTTGaaa           <  2:13103/143‑1 (MQ=255)
                                                                                                                                cTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGGAATCAAATTACTGTTCGCGATCTTGaaa           >  2:295818/1‑143 (MQ=255)
                                                                                                                                cTGCCAGGCAGCACATTAGCAAACCCCCTCTTGTTTAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGaaa           <  1:138273/143‑1 (MQ=255)
                                                                                                                                  gCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAGGc         >  2:76997/1‑143 (MQ=255)
                                                                                                                                     agacATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCaaa      <  1:398084/140‑1 (MQ=255)
                                                                                                                                     aGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCaaa      >  1:445826/1‑143 (MQ=255)
                                                                                                                                         aTCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTg  >  1:432099/1‑143 (MQ=255)
                                                                                                                                             |                                                                                                                                          
GGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTG  >  NC_000913/4213021‑4213300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.