| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 76,805 | T→A | 100% | E165D (GAA→GAT) | sgrR ← | DNA‑binding transcriptional dual regulator SgrR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 76,805 | 0 | T | A | 98.6% | 49.3 / ‑6.8 | 19 | E165D (GAA→GAT) | sgrR | DNA‑binding transcriptional dual regulator SgrR |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (9/9); minor base G (1/0); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGG > NC_000913/76678‑76937 | ttCGTTTGAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAACCGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCAttt < 1:36468/143‑1 (MQ=255) gcaaaGAGGCCACCACATCGGCCATTACCAGTACACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGACATGTTCTACCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCa < 2:110604/141‑1 (MQ=255) gATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCCGTGAAGCGGTGAACTTTGCTGCCAGCGGTGGGCGCTGTCTGCATCCAGTTCCCCGTCTCCCTCCTTTCTGcacg > 1:27663/1‑143 (MQ=255) tCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATATTCCCCATTTATGCGCTGTAGcta > 2:188194/1‑141 (MQ=255) aGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCGTTTATGCGCGTTAGCGAACTGAAGATTTg > 1:438803/1‑143 (MQ=255) gTTCACGATCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGc < 2:336437/143‑1 (MQ=255) aaaTGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAGCTCAACATTTGCCGGGCGAGATGGGTTTc > 2:233684/1‑143 (MQ=255) aCTCCTTGACGCAAAAAGTAACGCCAGTTAAGCGTTGAAATTTGCTGCCAGTGTTGTGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGTGCGATATGGGTTTCGGAACg < 2:427184/143‑1 (MQ=255) gCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACGGAAGAGTTGCCGGGCGATATGGGTTTCGGAACGGGGCGATgcg > 1:198651/1‑143 (MQ=255) aaaGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCGTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCACTGCGCTGc > 1:194335/1‑143 (MQ=255) gCCAGTGAAGCGGTGAAATTTGCCGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTCTGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGACCGGCGCAAGGCGCTGCCAGGTCGc > 1:291833/1‑143 (MQ=255) cAGTGAAGCGGTGAAATTGGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCTGGGAGATATGGGTTTCGGAAAGGAGAAATGGGCTGCAAGGAAGCAg > 1:323865/1‑143 (MQ=255) gAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTa < 1:306367/143‑1 (MQ=255) aaaTTTGCTGCCGGTGGTGGGCGATGTCGGCATCCAGTCCCCCATTTTCCTCATTTCTGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGa < 2:291075/143‑1 (MQ=255) aTTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTGGCAGATTACGCAACGGACg > 1:170751/1‑143 (MQ=255) tggtggGCGATGTCTGCATCCAGGTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCAc < 1:472724/143‑1 (MQ=255) ggtggGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGACAGGTAGCAGATTACGCAACGGACGACCGTGGAGcgcg > 1:88403/1‑143 (MQ=255) gggatagcTGCATCCTGTCCCCCTTTTTCCTCATGTATGCGCGTTAGCTAACTGAAGATTTGCCGCGCGATATGGTTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACAGACGATAGTAGAGCACGCGCAg < 2:380575/136‑1 (MQ=255) cGATGTCTGCATCCAGTTCCTCATTTTCCTTTTTTATGCGGGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAgg < 1:83941/143‑1 (MQ=255) | TTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGG > NC_000913/76678‑76937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |