| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,416,622 | C→G | 100% | Q626E (CAG→GAG) | pta → | phosphate acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,416,622 | 0 | C | G | 100.0% | 50.1 / NA | 19 | Q626E (CAG→GAG) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (13/6); total (13/6) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTG > NC_000913/2416488‑2416757 | gTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGAAGAGACGTCGAAAAAGTTCGCGAAGCAACTCGCCTGGCGCAGGAAAAACGTCAAGACCCGATGATCGACGGTCCGCTGGAGTccgac > 2:225448/1‑143 (MQ=255) cgTTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATgg > 1:103273/1‑143 (MQ=255) ttGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCCGTAATGGCt > 2:314254/1‑143 (MQ=255) tctcCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCCGGAGTACGACGCCGCGGTAATGGCTGACGTTGCg > 1:306748/1‑143 (MQ=255) tACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCCGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCGGCGGTAAAGGCCCACGTTGCGacggc > 2:171020/1‑139 (MQ=255) tCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCaa < 2:103273/143‑1 (MQ=255) tAGCGACGTAGAAAAAGTTCGCGAAGCAGCTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAg < 1:127135/143‑1 (MQ=255) cGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAggac > 2:394099/1‑141 (MQ=255) gTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGGGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCgcgc > 1:423566/1‑143 (MQ=255) gTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCgcgc < 1:47220/143‑1 (MQ=255) gcgAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATc < 1:447989/143‑1 (MQ=255) gAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGATCATCtt > 1:244349/1‑143 (MQ=255) gAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGAAATCGATAACTTTTCTAAATCCAAAGCGCCGAACTCTCCGGTTGCAGCTCGCGCGACCGCGTTCATcgt > 2:310171/1‑141 (MQ=255) gcgcAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCAACTTCCCGGACCTGGaccc > 2:222802/1‑141 (MQ=255) cAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCTAACACTCCTTACTGTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGGTCTGAACACCgg > 1:237658/1‑143 (MQ=255) gTCCTGACCAGATGCTTGACGGTCCGCTGGAGTGCGGCGCTGCGGTAATGGCTGACGTTGCGAAATGCAAAGCGCCAGACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCTGATCTGAACACCGGTAACaccacc < 1:51283/143‑1 (MQ=255) tCCTGACCTGATGATCGACGGTCAGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGCACTCTCCGGTTGCAGGTCGCGCTACCGTCTTCATCTTCCCGGATCTGACCACCGGTAACaccaccc > 2:512172/1‑142 (MQ=255) acctgaTGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACGCCGGTAACACGACCTAaaaa > 1:425941/1‑143 (MQ=255) tCCGCGGGAGTACGACGCTGGGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTCCCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTg < 2:237213/143‑1 (MQ=255) | GTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTG > NC_000913/2416488‑2416757 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |