Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,041,398	T→G	100%	L29R (CTT→CGT)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,398	0	T	G	100.0%	81.7 / NA	27	L29R (CTT→CGT)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (14/13);  total (14/13)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACG  >  NC_000913/3041258‑3041533                                                                                                                                             |                                                                                                                                        aTAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTg                                                                                                                                       >  2:399663/1‑143 (MQ=255)                    tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGa                                                                                                                    <  1:380048/143‑1 (MQ=255)                                  ttAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCGCCCCGTCAGCCTACGGCTTCTGCCCGTTTCCCGCTGACGGTGATGACGCTTGATGACTAGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAatata                                                                                                      <  2:211591/143‑1 (MQ=255)                                                                   acCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATCTGCAGGGTCAGGTGACAGCAGATGTCAGCCAGa                                                                     >  1:332070/1‑143 (MQ=255)                                                                   acCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCGGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGTAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCgaga                                                                     >  1:143699/1‑143 (MQ=255)                                                                        ttCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCa                                                                <  1:399663/143‑1 (MQ=255)                                                                        gtcctccCCGGCAGCCTACGGCTCCTGCCCGTTTGCCGCTGACGCTGATGACGCTTGATGACGGGGCGCGTGCGACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCa                                                                <  1:33056/142‑1 (MQ=255)                                                                         tcctccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGCCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCGGGTGACAGCAGATGTCAGCCAGATCGctg                                                               >  1:232230/1‑141 (MQ=255)                                                                            tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACCGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaag                                                            >  1:111539/1‑143 (MQ=255)                                                                            tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGCCAGCAGATGTCAGCCCGGTGCCagaag                                                            >  1:14248/1‑143 (MQ=255)                                                                              cccGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGAGGTCAGCCAGATGGccagagcc                                                          >  2:24359/1‑136 (MQ=255)                                                                              cccGTCAGCCTACGGCTTCTGCCCGTTTACCACTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGAt                                                          <  1:190672/143‑1 (MQ=255)                                                                                 gTCAGCCTACGGCTTCTGCCCGTTGAGCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATAGGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg                                                       <  2:40296/143‑1 (MQ=255)                                                                                   cAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa                                                     <  2:147783/143‑1 (MQ=255)                                                                                           ggCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcg                                             <  1:133021/143‑1 (MQ=255)                                                                                                    cggTTTGCCGCTGAGGGTGGTGGCGCTTGATGACTGGGCGCGGGGCAGCAGTACTGGCGCGGACAGCGAAGAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCAtt                                    <  2:126062/141‑1 (MQ=255)                                                                                                         tACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCCCCTGCTCGCCGCCCATTGCGAc                               >  1:175351/1‑143 (MQ=255)                                                                                                          aCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCCCGCCGCCCATGGCGACg                              >  2:186871/1‑143 (MQ=255)                                                                                                              ctgacactgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCaa                          <  1:445040/143‑1 (MQ=255)                                                                                                               tgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGAT‑AGCACCTGCTCGCCGCCCATTGCGACGCCACAg                        >  1:34864/1‑143 (MQ=255)                                                                                                                gacgcgggTGACGCTTGCTGACTGGGCGCGTGCCACCATTACTGGCGAGGACAGCGAAAAAGATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGAGGCCAAAg                        <  2:374940/143‑1 (MQ=255)                                                                                                                        tgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATCGCGACGCCAAAGATAAACtg                >  2:490373/1‑143 (MQ=255)                                                                                                                         gaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtgt               <  1:294973/143‑1 (MQ=255)                                                                                                                          aCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAACCATGTCTGCACGTTCAGGTGACAACAGATATCAGCAAGATGACAGAAGATCAACACCTGATCGCCGACCATTGCGACGCCAAAGGTAAAAggtg              >  1:311420/1‑143 (MQ=255)                                                                                                                             ctcgatgaCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAg           <  1:121160/140‑1 (MQ=255)                                                                                                                                  tgaCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATGTATGCAGGGTCAGGTGGCAGCAGATGGCAGCCAGATGGCAGAAGATCAGCCCCTGCGCGCCGCCCATTGCGACGCCACAGGTACAATGTGGAGCaagt      >  1:33620/1‑141 (MQ=255)                                                                                                                                      tGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACg  >  1:393549/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                        ATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACG  >  NC_000913/3041258‑3041533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑