| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,140,352 | G→C | 9.9% | R150G (CGC→GGC) | hybE ← | hydrogenase 2‑specific chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,140,352 | 0 | G | C | 9.9% | 93.5 / 2.9 | 37 | R150G (CGC→GGC) | hybE | hydrogenase 2‑specific chaperone |
| Reads supporting (aligned to +/- strand): ref base G (16/17); new base C (2/2); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTG > NC_000913/3140217‑3140490 | gcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCACAGAGACAACTCATGCCTTCTCGCCACCCCTGCGCCCACACAGCAG‑GGCCCCACGcccc > 1:402009/1‑142 (MQ=255)gcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGGCGCCCt > 1:228283/1‑143 (MQ=255) actggccAGCCACACGGCGGTGACGCGCTTAACGTCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATGTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGTCGCGCCCTGct < 2:99662/138‑2 (MQ=255) aaTTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCGCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGTCGCGCCCTGct < 2:99696/143‑2 (MQ=255) cacGGCGGTGACGCGCTTATCTTCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATcc < 1:247813/143‑1 (MQ=255) aCGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGa > 2:327034/1‑143 (MQ=255) aCGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGa < 1:382527/143‑1 (MQ=255) cGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCCGCGAAAg > 2:44023/1‑143 (MQ=255) tgatcctccTGCCGTTGGATAATTTCAACGTCGGTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCa < 2:73077/140‑1 (MQ=255) ccGCCTGCCGTTGGATAAATTCAACGGCGCACTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCg > 2:63725/1‑143 (MQ=255) gCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACGGGCAGCGAAAGGAACATTCGTGCGCa > 2:380451/1‑143 (MQ=255) gATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGCGCGCAGTCATcc > 2:138805/1‑143 (MQ=255) actttCACCGCCGCCCTGGCAAAGAGACCACTCCTGCATTTTCGCCACTCCTGCGCCCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTc < 1:425883/141‑1 (MQ=255) aaTCTCAACGGCGCTCGGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTc < 2:6876/143‑1 (MQ=255) ttCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGCTCATTCGTGCGCAGTCATCAGACAgg > 1:91684/1‑143 (MQ=255) gcgcTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACGAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGc > 1:495406/1‑143 (MQ=255) gcgcTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGc > 1:497463/1‑143 (MQ=255) tctGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGGCGGCCctc > 2:469604/1‑143 (MQ=255) ctGGCAAAGAGACAACTGATGCATGTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGTCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCctct < 2:430609/143‑1 (MQ=255) ctGGCAAACAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGACCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGGCctct < 2:430626/143‑1 (MQ=255) gCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCa < 1:367727/143‑1 (MQ=255) cgagaacaCTCAGGTATTTTTGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCg < 2:15875/136‑1 (MQ=255) aCTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCGCTTCAATCGACATGCt > 1:410127/1‑143 (MQ=255) tCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCtgt > 1:338317/1‑143 (MQ=255) cATGCATCTGCGCCGCGCCTGCGACCAACCACCAGAGCCGCGATGTCGT‑C‑GTGTGGTACAGCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCtgtg < 2:501606/143‑1 (MQ=255) ctCGCCACTCCTGCGACCAACCAGCAG‑GGCGCGACGCCCTGC‑GGGGGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgcg < 1:431052/142‑1 (MQ=255) ctcccttCCTGCGACCACACAGCAG‑GTCGCGTCGCCCTGC‑TTGTGGTACATACGGATTGGTGACTGGCGTCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgcggc < 2:212608/137‑1 (MQ=255) cggCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAg < 1:410327/141‑1 (MQ=255) cTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAg < 1:359522/143‑1 (MQ=255) tGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGc < 1:327034/143‑1 (MQ=255) gACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGaa > 2:38735/1‑143 (MQ=255) cAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAAccg > 2:450110/1‑141 (MQ=255) cacacgcag‑GGCGTCGCGCCCTGCTGCTTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTCCAATCGACATGCTGTGCGCAAGCGGCGCCATCAGCGAACAg > 1:195515/6‑143 (MQ=255) aaCAGCGG‑GGCGCGACGCCCTGC‑GTGTGGTACATCGGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTGCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGga < 2:463052/143‑1 (MQ=255) ggCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAGAGCGGCGACATCAGCGAACAGGAGAGATAtt > 2:15268/1‑143 (MQ=255) ggCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGACCAGGAGAGCTAtt > 2:461538/1‑143 (MQ=255) gcgcGGCGCCCGGC‑GTGTGGTACATCCGGATTCGTGGCTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTg < 1:132550/143‑1 (MQ=255) | GCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAG‑GGCGCGACGCCCTGC‑GTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTG > NC_000913/3140217‑3140490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |