| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 87,481 | C→A | 100% | T42K (ACA→AAA) | ilvH → | acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 87,481 | 0 | C | A | 95.9% | 33.2 / ‑6.2 | 15 | T42K (ACA→AAA) | ilvH | acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (3/11); minor base G (1/0); total (4/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GAGAACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCGGGAAATCATGCTG > NC_000913/87345‑87620 | gactaCCTGATTATGCGCCGGATATTATCAGTCTTTCTCGAAGCTGAATCAGGCGCGTTATCCCGCGTTATTGGCCTTGTTTCCCAGCGTGGCTACCACATTGAAAGCCTGACCGTTGCGCCTACCGACGATCCGAAATTATc < 2:42595/139‑1 (MQ=255) gaACCTGAGTAGGGGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATcgc < 2:275986/143‑1 (MQ=255) aaCCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCATTTTTTCCCAGCGTGGCTCCAACAATGAAAGCCTGACCGTTGGACCCACCGAGGGTCCGAGATTAGcgcg > 2:357037/1‑143 (MQ=255) ttATGCGCCGGTTATTAGCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTTAAAGCCTGACCGTCGCGCCAACCGACGATCCGAAATTATCGCGTATGAcc < 2:286551/143‑1 (MQ=255) ggacGGATAGTATCGGTCTTACGGGCATGTGAATCTGGCGCGTTTTGCCGGGTGATTGGCCTTTGTTCTCAGCGTGGCTAGAACATTGAAAGCATGTCCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCa < 2:245735/140‑1 (MQ=255) tattatCAGTCCTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTg < 1:126545/143‑1 (MQ=255) gtcctccTCGAAAACGAACCAGGCGCGTTATCCCGTGCATTATTCGTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGa < 2:226838/137‑1 (MQ=255) aaaaTGTAGCAGGCGCGTTATTCCGCGTGATTGGCCTTTTGTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACtt < 2:14080/143‑1 (MQ=255) cAGGCGCGTGATCCCGCGTGCTGGGCCTCGCTGTCCGCCGTCGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATc < 1:487915/143‑1 (MQ=255) gATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTCGCGCCAACCGACGATCCGAAATTATCGCGTATGACCAGCCAGACCGTGTGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAAc < 2:518517/143‑1 (MQ=255) tACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGCATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAGCTGGTCGATGTCTGGCGCgtgagtga > 1:195130/1‑143 (MQ=255) aacaTTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTGTGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGAGTGAGTGAGtt > 2:232709/1‑143 (MQ=255) aacatcacgcCTACCTACGATCCAAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCa < 1:188130/136‑1 (MQ=255) ttGAGCCAGCCGACGATCCGAAATTAGCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGtt < 1:475447/143‑1 (MQ=255) cGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCGGGAAATCATGCTg > 1:160619/1‑143 (MQ=255) | GAGAACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCGGGAAATCATGCTG > NC_000913/87345‑87620 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |