| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,296,060 | C→T | 27.7% | intergenic (+266/+376) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,296,060 | 0 | C | T | 27.7% | 56.5 / 29.5 | 62 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Reads supporting (aligned to +/- strand): ref base C (22/22); new base T (14/3); total (37/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.02e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
GGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATC > NC_000913/4295921‑4296202 | ggCGTTGATGTCGGATGCGGCATAAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATAAACGcc > 1:434762/1‑143 (MQ=11) tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGCCATCAACGCCTGAt > 1:489235/1‑143 (MQ=17) tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGAt < 1:98297/143‑1 (MQ=32) tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGAt > 1:348219/1‑143 (MQ=32) ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAATGCCTGATGCGACg > 1:409796/1‑143 (MQ=17) cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAATGCCt > 2:47672/1‑133 (MQ=17) cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATCTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAATGCCt < 1:47672/133‑1 (MQ=16) ggaTCCGACATGAGCGCCTGGTGCGAAGCATAACGCGTCTTATCAGGACTACGACAGACAGTGCTATAGCCGTATTTAGAGTTATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCt < 1:118323/141‑1 (MQ=11) cATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAATGCCTGATGCGACGCtt > 1:262657/1‑143 (MQ=17) aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTCCGCCAGACAGCGCGATAGCCTGATTTAGCGTGGTCTTGGAGGTCCGCTAAGGCGTTCACGCCG‑CATCCGACATCAACGCCTGATGCGGCGCTTg > 2:351853/1‑143 (MQ=11) tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTAATTTTGTAGGTCGGCTTAGGCGTTTACGCCG‑CATCCGACATCAATGCCTGATTCTACACTTGc > 1:201149/1‑143 (MQ=11) cGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATACCCTGCTTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAATACACGATACGACGCTTAcca > 1:23310/1‑142 (MQ=11) gACGTCCACGCCTGACGCGACGCACACCGCGTCTTACCAGCCCGACGCCACACAGCGCAATCGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCcgc < 1:170297/143‑1 (MQ=11) gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAATGCCTGATGCGACGCTcgccgc > 1:91807/1‑143 (MQ=21) gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCCCCTTTAGCGTGATCTTCTAGGTCGGATAAGGCCTTTACGCAG‑CATACGACATCAATCCCTGACGCGCCGCTGGccac > 2:427257/1‑141 (MQ=11) cgtCAACGCCTGATGCGACGTTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt < 2:245410/141‑1 (MQ=255) aTCAACGCCTGATGCGACGCTTACCGCGTCTGCTCAGGCCCACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTc < 1:94809/143‑1 (MQ=255) aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc > 1:468670/1‑143 (MQ=25) tCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTCGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAATGCCTGATGTGACGCTTGCCGCGACt > 1:258981/1‑143 (MQ=255) gtagcGCCTTGTGCGGCGCGTAACGGTTCTTATCAGGCCTCCGCCAGAGGGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCt < 2:116394/139‑1 (MQ=11) cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGAGGACCTTC‑ACATCAAGGCCTGATGCGACGCTTGCCGCGTCtt > 2:225966/1‑143 (MQ=11) aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGCCATCAACGCCTGATGCGACGCTTGCCGCGTCttt > 1:454619/1‑142 (MQ=37) atgCCTGATGCGACGCTTAACGGGTCTTATCAGGGCTACGCCAGACGGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt < 2:344393/141‑1 (MQ=255) gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATcc > 2:327705/1‑142 (MQ=25) gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa < 1:351853/143‑1 (MQ=25) cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAATGCCTGATGCCACGCTTGCCGCGTCTTATAAgg > 2:240531/1‑143 (MQ=255) gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc < 2:348219/143‑1 (MQ=38) aTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt > 1:394308/1‑143 (MQ=37) cGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc > 1:477549/1‑141 (MQ=18) aCGCTTTACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGACAAGGCGTTTACGCCG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctt > 2:133948/1‑139 (MQ=255) gCTTAACGCGTCTTATCAGGCCTATGCCAGCCAGCGCATAAGCCTGATATAGCGTGATTTTGTAGGTCGGACACGGCGTTTACGCAG‑CACCGGACATCAACGCGTGATGCGACGCTTACCGCGCCTTATCAGGCCTCCGACAg > 2:431726/1‑143 (MQ=11) ttAACGCGTCTTATCAGGCCTACGCCTGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATACACGCCTGATGCGACGCTTGCCGCGCCTTATCCGGGCTACGTCaacc > 2:247839/1‑140 (MQ=11) tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc < 1:273733/143‑1 (MQ=39) tCAGGGCGACGCGAGACAGCGAAATAGCAAGACTGAGCGTGATTTAGTAGGGCGGATAGGGCGTTTCCGCCG‑CGTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCt < 2:507005/143‑1 (MQ=11) aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGCCTTATCAGGCCTACCCCAGCCGTCGCATAAGCCTgc > 2:387696/1‑142 (MQ=11) cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa < 1:139355/143‑1 (MQ=39) gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCATCGCAACAGCCAGAttgggcga > 2:125210/1‑137 (MQ=11) taCAGCGTCATAGCCTGATTTAGCGCGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtt < 1:509316/142‑1 (MQ=255) gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CACCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtt < 1:390287/143‑1 (MQ=255) cAGCGCAATAGCCTAACTCAGCGTGATGTTGTAGGGCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtttt < 1:121775/143‑1 (MQ=11) aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCtt > 2:365748/1‑95 (MQ=255) aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCtt < 1:365748/95‑1 (MQ=255) ccccAATAGCCAGACTTGGCGTGAGTTTGTATGGCGGATAGGGCGTTTCCGCCG‑CGTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt < 2:52645/140‑1 (MQ=11) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCTCAGACAGCGCTAGATCCTGACTTAGCGTCAttcccgg > 2:210438/1‑138 (MQ=11) gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa > 2:168573/1‑123 (MQ=17) gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa < 1:168573/123‑1 (MQ=40) tGATTTTGTAGGGCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGTCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATg < 1:171336/143‑1 (MQ=255) tAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCATTACCCTGATATAGCGTGACTATCTATGTCAGAACAAGCGCTTCTGCCGCATct > 2:250535/1‑142 (MQ=11) ggTCGGATAAGGCGTTTCCGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGa < 1:180220/143‑1 (MQ=38) ggTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGa < 1:422192/143‑1 (MQ=39) aaGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACg > 1:290472/1‑143 (MQ=38) ggCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCCATAGCCTGATTTAGCGTGATTTTGTGGGTCGGCTACGGCGTTTATGCCGCATCCGACCTCCCCGcc > 1:134978/1‑143 (MQ=11) gTTTACGCCG‑CATCCGACATCAACGACTAATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCACAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGCCATCCACGCCTgc > 1:355877/1‑142 (MQ=11) gccg‑cATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATGAACGCCTGATGCGAc > 1:508850/1‑143 (MQ=25) gccg‑cATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAGCCAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATACGGCGTTACTGCCGCATCCGACATAAACGCCTGATGCGAc > 2:40529/1‑143 (MQ=11) cg‑cATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATTCCACGc > 2:61107/1‑143 (MQ=11) aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATGAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGGCGCttt > 2:377767/1‑142 (MQ=12) tCCGGCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa < 1:451632/143‑1 (MQ=25) tCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCGTCCGACATCAACGACCGATGCCACCCttca > 2:334054/1‑141 (MQ=11) cGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGACTTTCTAGGTCGGATAAGGCGTTTATGCCGCCTCCGACATCAACGCCTGATCCGACGCTTAAcg > 2:239425/1‑143 (MQ=255) tcggaaCGCCGGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGt < 2:327942/139‑1 (MQ=14) cAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAg < 1:87645/91‑1 (MQ=34) cGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATc > 1:91290/1‑143 (MQ=11) | GGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATC > NC_000913/4295921‑4296202 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |