| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,426,592 | T→A | 7.1% | intergenic (+35/+36) | rplI → / ← yjfZ | 50S ribosomal subunit protein L9/protein YjfZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,426,592 | 0 | T | A | 7.1% | 150.7 / 3.9 | 59 | intergenic (+35/+36) | rplI/yjfZ | 50S ribosomal subunit protein L9/protein YjfZ |
| Reads supporting (aligned to +/- strand): ref base T (36/15); new base A (2/2); total (39/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.93e-01 | |||||||||||
TCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCT > NC_000913/4426455‑4426728 | tCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTc > 2:344578/1‑143 (MQ=255)tCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAACGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAACAACGCCGACCATTCGTc > 2:367899/1‑143 (MQ=255) tGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTACAAACGCCGACCAGTGGTCgg > 2:28619/1‑143 (MQ=255) cGAACGGCGTTGTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCAATGGTCGGCGt < 2:80954/143‑1 (MQ=255) cGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCAATGGTCGGCGt < 2:343084/143‑1 (MQ=255) aaCGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGttt < 1:356290/143‑1 (MQ=255) tGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCtgt > 1:256625/1‑141 (MQ=255) aCCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCCGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTc > 1:515437/1‑143 (MQ=255) cACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGt > 1:505171/1‑143 (MQ=255) cACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTGATTCAGCGAGACGTAACAACGCCGACCATGGGTGGGCGTTTTGCTTTCTATTCTTCgc > 1:357623/1‑142 (MQ=255) cGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCGTTCTATTTTACGTCAGGTa > 1:204324/1‑143 (MQ=255) gAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAgggag > 1:336736/1‑140 (MQ=255) gAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTAAAACAGAATGGGCAGGAAGGCCGAACATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTAt > 1:377030/1‑143 (MQ=255) acacGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTa < 1:426396/143‑1 (MQ=255) acGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGGTCGTAAACGTAGTAGCTGAATAATTCGTTATTCCACGAGACGTAAAAACGCCGACCATAGGTCGGCGCTTTGCATTCTATTTTTCGTCAGGTATTaat > 2:418128/1‑141 (MQ=255) acGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCGATTTTTCGTCAGGTATGAGt > 1:174937/1‑143 (MQ=255) aggTGAGCTGCCCAGTTCACCGCGAAGTACTCGAGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTc < 1:224293/141‑1 (MQ=255) gTGAGCATGCAGGTTCACAGCGAAGTATGCGCGAAAGTGAGCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGc < 2:324246/143‑1 (MQ=255) tgtgCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCa < 1:453115/140‑1 (MQ=255) aGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGATTTCTATTTTTCGTCAGGTATTAGTTTCGCAAg > 2:49821/1‑143 (MQ=255) cTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTa > 1:23130/1‑143 (MQ=255) ttCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCGGGTAGTAGTTTCGCCAGTAg > 2:409573/1‑143 (MQ=255) ccAGGTTCACCGTCAAGTATTCGCGAATGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTGAACGGGACGTAACAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGAt < 2:450359/143‑1 (MQ=255) ggTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGAAATAGCTGAATAATTAGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTATTGCTTTCTATTTTTAGTCAGATATTAGTTTCGCACGTAGATCCa > 2:36732/1‑143 (MQ=255) cacaGCGAAGTACTCGCGCAAGTGATCGTAAACGTAGTAGCTGAATAGTTCGTTATTCAACGAGACGTAAAAACGCCGACCATCGGTCGGCGTTATGCGATCTATCAGTCGGCAGGGATTAGTCACGCAAGTCTATCCacaca > 2:328625/1‑139 (MQ=255) aGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTGTTTTTCGTCAGGTATTAGTTTCGCGAGTAGATCCATTCACCa > 2:383942/1‑143 (MQ=255) ttCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATGCTCTtgt > 1:348480/1‑143 (MQ=255) gcgcgAAAGTGATCGTAGAGTAAGAAGCTGAATAATTATTTATTCAAGGAGACGTAATAACGCCGACCAGTGGTCGGCGTCTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTtgtg < 2:129225/142‑1 (MQ=255) ggTGACCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATaa < 2:465435/142‑1 (MQ=255) aGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAACCGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATaa < 1:197629/143‑1 (MQ=255) aGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATaa < 1:443883/143‑1 (MQ=255) gATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCCCCATTCTCTTGCGTCCATaacgc > 2:240398/1‑140 (MQ=255) gATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTATCTTGTGTCCCTAAGTc > 1:11262/1‑143 (MQ=255) aTCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAAGAGATCCATTGACCACTCTCGCGTGTCCATacgcca > 2:469180/1‑138 (MQ=255) tCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATCAGGCAg > 2:24198/1‑143 (MQ=255) tAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCACGTAGATCCATTCACCATTCTCTTGTGTCCATCAGTCAGGAt > 1:451473/1‑143 (MQ=255) cGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGtt > 1:203676/1‑143 (MQ=255) cGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTATTGCTTTCTATTTTTCGTCAGGTATTAGTTTAGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATACGTCAGGATGGtg > 2:122846/1‑142 (MQ=255) tagtagCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATAGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGCCAGGATGGTTTg > 2:360237/1‑143 (MQ=255) gCTGAATAGTTCGTTATCCAACGAGACGTAAAAACTCCGACCACTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCTTTGTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGc < 2:341857/143‑1 (MQ=255) gAATAATTGTTTATTGAAGTAGTGGTAAAAACGCTGTCTATTTGTCGGTGTTTTGCTTTCTATTTTTGGTCAGGTATTAGTTTCGTAAGTAGATCCATTCACCATTCTCTTGTGTTCATAAGTAAGGATGGTTTGCTGGCGtt < 2:420205/143‑1 (MQ=255) gAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGtt > 1:243390/1‑143 (MQ=255) aatgatTCGTTATTCAACGAGACGTAAAAACGCGGACCATTGGTCGGCGTTGTGCTTGCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGGTGGTTTGCTGGCGGTa > 2:257647/1‑143 (MQ=255) aataatTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGGTTGCTGGCGTTa > 1:386019/1‑143 (MQ=255) taatTCGTTATTCAACGAGACGTAAAAACGCCGTCCATTGGTCGTCGTTTTGCTTTCTTTTTTTTGTGGTCGCAGAGAAACACATGTCGGCTGACTCAGCTTTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGa < 1:516192/143‑1 (MQ=255) taacTCGTTAGTCAACGAGACTTAAAACCGCCGACCAGTGGCCGGCGTTTTGCTTTCTATTTTTCGTCAGGCATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGa < 1:498765/143‑1 (MQ=255) ttCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGatac < 1:163041/143‑1 (MQ=255) ttCGTTATTCAACGAGACGTAAAAACGCCGACCAATGGGCGCCCTATTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGTCGTTGGgtac > 1:241724/1‑143 (MQ=255) ggtgaTTCAACGCGACGTAAACACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGCTTCGCAAGTAGATCCATTCACCATTCTCTGGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGatacat < 2:445401/139‑1 (MQ=255) gTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGatacata > 1:54691/1‑143 (MQ=255) ttCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAg > 1:310496/1‑143 (MQ=255) aaCGAGGCGTAAACACGCCGACCATCGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAgcgc < 1:93782/143‑1 (MQ=255) gaCGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGAGCAATTCACCATTCTCTTGTGTCCATAAGTAAGGATGGTTTGCTGGCGTCAGCTACATAAGAGAGTAACTg > 2:17159/1‑143 (MQ=255) aCGTAAAAACGCCGACCATTTGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGa < 1:353904/143‑1 (MQ=255) aaaaCGCCGACCAATGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGtct > 1:262729/1‑143 (MQ=255) aaaCGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCCTAGGTCAGGATGCTTTGCTGGCGATAGATACATACGAGCGCAACTGATGtctc > 2:203814/1‑143 (MQ=255) aaCGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGGCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCg > 1:355192/1‑143 (MQ=255) gCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGCTACATACGAGCGCCACTGCTGTCTCGCTc > 1:134936/1‑143 (MQ=255) cGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCCCCATTCTCTTGTGTCCATCATCCAGGATGGTTTGCTGGCGTTCGATACATACGAGCGCAACTGATGTGCCGCTCCt > 2:59916/1‑143 (MQ=255) | TCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCT > NC_000913/4426455‑4426728 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |