| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 710,595 | G→T | 34.0% | P18T (CCT→ACT) | fur ← | DNA‑binding transcriptional dual regulator Fur |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 710,595 | 0 | G | T | 34.0% | 13.5 / 14.2 | 24 | P18T (CCT→ACT) | fur | DNA‑binding transcriptional dual regulator Fur |
| Reads supporting (aligned to +/- strand): ref base G (8/8); new base T (4/4); total (12/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.53e-01 | |||||||||||
AAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCC > NC_000913/710461‑710737 | aaaCTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGGTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTa < 2:406592/143‑1 (MQ=255) aaCTGTTTCAGTACGCGCTATTCCGTAGCCAGACCCATTTCTTCACCCATATCGATCAGACGTTTGTGTAAATCTTCCGCACTGACGTGTTGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTAc < 1:71591/143‑1 (MQ=255) aCTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACt < 1:360344/143‑1 (MQ=255) gTTCAGTACGCGCTACCCCGCTTGTAGATCAATTTCGTC‑CCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGCACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAg < 2:385635/143‑1 (MQ=255) gTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATACATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGCAGCCCCTCCAGGACTTTTCAACGAGTACGCGTTACTTTCa > 2:511836/1‑143 (MQ=255) ttCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAg > 1:312034/1‑143 (MQ=255) ggaGCGAGACCAGTTTCGTCACCCGTATCGATCGGACGTTTGGATAAGTCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACGTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGc < 1:36187/141‑1 (MQ=255) cAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCCGCTCTCTTTAGGGCGGTATGGTTa > 1:173118/1‑143 (MQ=255) cAATTTCTTCACCCATATCGATAAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGttg > 1:99415/1‑142 (MQ=255) tttCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCCGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCa > 2:122665/1‑143 (MQ=255) tcACCCATATCGATAAGACGTTTGTATAAATCTTCCGTACTGACGTGATGGTTGTCAGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTAATTTCAGGCGAGCTTTCTTTAGGGGGGTATTGTGATCAGTCAt > 2:318371/1‑143 (MQ=255) aTCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTATACGAGGAATCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTc > 2:33798/1‑143 (MQ=255) gTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTGTGGGGCGGTATTGTTATCAAACAGGCGGGAGCTGTCCTGTTAc > 1:69113/1‑143 (MQ=255) gTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTGTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTCTTGTTATCAGTCATGCGGGATCTGTCCTGttcc > 1:168608/1‑141 (MQ=255) taAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCgg < 1:474568/143‑1 (MQ=255) cACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCAtt < 1:133344/143‑1 (MQ=255) gcTGTCGTGATGGTTGTACGGCTCCTGAAGAACTTCCAGGATTTTTGAACGAGGAAGCGTTACTTTCAGGCCAGCTTTGTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTa < 2:171386/142‑1 (MQ=255) aCGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTagaag < 1:208056/143‑1 (MQ=255) gTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGACTCTGGCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGaa > 1:490004/1‑143 (MQ=255) tGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTg > 1:360940/1‑143 (MQ=255) aagaaCTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTgag < 1:414171/143‑1 (MQ=255) tCCAGGATTTTTCTACGAGGAAGCGTTACTTTCAGTGCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGc < 2:300206/143‑1 (MQ=255) tCCAGGATTTTTAAACGAAGAAGCGCTACTTTCAGGCCAGCGTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGc < 2:43119/143‑1 (MQ=255) cAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATACTGCGt > 1:489915/1‑143 (MQ=255) gAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGcc < 1:326618/143‑1 (MQ=255) | AAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCC > NC_000913/710461‑710737 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |