Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 76,805 T→A E165D (GAA→GAT)  sgrR ← DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091376,8050TA97.7% 124.8 / NA 43E165D (GAA→GAT) sgrRDNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (24/18);  minor base C (1/0);  total (25/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGTATTGATTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCC  >  NC_000913/76667‑76944
                                                                                                                                          |                                                                                                                                           
cAGCTTATTGATTCGTTTTAAAGAGGCGATCACCTCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACGCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCa                                                                                                                                         <  2:807891/143‑1 (MQ=255)
 ggCGGATTGATCCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAg                                                                                                                                        <  2:703210/142‑1 (MQ=255)
              gTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCTCCATTTTcc                                                                                                                           >  1:447599/1‑143 (MQ=255)
               ttttAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCCGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGCTGTCTGCATCCAGTTCCCCATTTTCCt                                                                                                                          >  2:787442/1‑143 (MQ=255)
                      gagGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGCCTCCTGGACGCCAAAAGATACGCCCGTGTAGCGGTTAAATTTGCTGGAAGTGGTGGGCGATTTCTGAATCCAATTCACCATTTTCCTCCttttt                                                                                                                   >  1:231867/1‑141 (MQ=255)
                      gagGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCGGTGAAGCGGTGAAGTTTGCTGCCCGTGGTGGGCGGGGTCTGCATCCAGTTCCCCATTTTCCTCCTTTAt                                                                                                                   >  1:443500/1‑143 (MQ=255)
                       agGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATg                                                                                                                  >  1:173721/1‑143 (MQ=255)
                           gATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGcgcg                                                                                                              <  2:673861/143‑1 (MQ=255)
                             tCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGGAAAAAGAAACGCCAGTGAAGCGGTGACATTTGCTGCCAGCGGGGGGCGATGTCTGCATCCAGTTCCCCATCTTCCTACGCTATGCGCGtt                                                                                                            >  1:179807/1‑143 (MQ=255)
                                caTCGTCCATTTCTGGTTCGCGACCGTGGTGAGAGTGGACTCCTGGACGCAAAAAGAAACGGCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGc                                                                                                         <  2:910678/143‑1 (MQ=255)
                                  tCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGACATTTGCTGCCAGAGGACGGCGATGTCTGAATCCAGTTCCCCATCCTCCTCATCTCTGCGTGTTAGCGa                                                                                                       >  2:28769/1‑143 (MQ=255)
                                   cGTGCATTTCCAGTTCACGACCATGGTGAGAATGGGCTCCTGGACGCAAAAAGAAACGCCAGTGACGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGaa                                                                                                      <  2:661817/143‑1 (MQ=255)
                                   cGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTGCCTCATTTATGCGCGTTAGCGaa                                                                                                      >  2:170455/1‑143 (MQ=255)
                                      ccATTTGCAGTTCACGACCAGGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTg                                                                                                   <  2:440816/143‑1 (MQ=255)
                                         tttCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAg                                                                                                >  1:51694/1‑143 (MQ=255)
                                             cAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGGGAACCGAAGCttt                                                                                            >  1:142188/1‑143 (MQ=255)
                                             cAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGACGAttc                                                                                            >  2:362673/1‑142 (MQ=255)
                                              aGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGACATTTACTGCCAGTGGTGGGCGATGTCTGCCTCCAGTTCCCCATTTTCCTCACTTATGGCCCGTAGCGCACTGACGATCTg                                                                                           >  2:455930/1‑143 (MQ=255)
                                                           ggggAAAATGGACTGATGGGCGCAAGAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATgg                                                                              <  2:448063/140‑1 (MQ=255)
                                                           ggTGCAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATgg                                                                              <  1:88519/143‑1 (MQ=255)
                                                            cgcgcaaTGGCCTCCTGGGCGCACAAAGAACCGCCAGTGCAGCGGTGAAATGTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATggg                                                                             <  2:179807/138‑1 (MQ=255)
                                                                        tCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGgc                                                                 >  1:60867/1‑143 (MQ=255)
                                                                         ccTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGgcg                                                                <  1:181872/143‑1 (MQ=255)
                                                                          cTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCACATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGAGCGATAAGGGTTTCGACACGgcgc                                                               >  2:506908/1‑143 (MQ=255)
                                                                              aCGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGGAGATTGGCCGGGCGATAGGGGTTTCGGAACGGCGCAATg                                                           >  2:282286/1‑143 (MQ=255)
                                                                                  aaaaaGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTATCGGAACGGCGCAATGCGCt                                                       >  2:242033/1‑143 (MQ=255)
                                                                                   aaaaGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTg                                                      >  1:779982/1‑143 (MQ=255)
                                                                                       gAAACGCCAGTGAAGCGGTGCAATTTGCTTCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTGAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAg                                                  <  1:115624/143‑1 (MQ=255)
                                                                                        aaaCGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAgg                                                 <  1:345451/143‑1 (MQ=255)
                                                                                          aCGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCAGCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTa                                               <  1:646183/143‑1 (MQ=255)
                                                                                                gTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGAt                                         >  2:889326/1‑143 (MQ=255)
                                                                                                     gCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGc                                    <  2:241015/143‑1 (MQ=255)
                                                                                                       ggTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATGACGCaa                                  >  2:716794/1‑143 (MQ=255)
                                                                                                        gTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTAATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATGACGCaag                                 >  1:267269/1‑142 (MQ=255)
                                                                                                            aaTTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGAc                             >  2:685674/1‑143 (MQ=255)
                                                                                                                     ccAGTGGCGGCCGCTGCCTCCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTCAGCGAACTGAAGATTTGCCGGGCGACATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTaga                    <  1:54545/143‑1 (MQ=255)
                                                                                                                       aGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCACCGGACGATAGTACAGc                  >  1:635220/1‑143 (MQ=255)
                                                                                                                           atggGCGATGTCCGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTATCGAACTGAAGATTTGCCTGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCAcgc              <  2:423689/142‑1 (MQ=255)
                                                                                                                              ggCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTGGAGCACGCGCa           >  1:885483/1‑143 (MQ=255)
                                                                                                                                cGAGGTCTGCAGCCAGTTCGCCATTTTCCTCGCTTATGCGCGGTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAgg         <  1:333291/143‑1 (MQ=255)
                                                                                                                                    gTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGAtgt     <  1:834239/143‑1 (MQ=255)
                                                                                                                                     tCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGCCGATAGTAGGGCACGCGCAGGAtgtg    >  2:506821/1‑143 (MQ=255)
                                                                                                                                       tGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCCACGGACGATAGTAGAGCACGCGCAGGATGTgcc  >  1:875870/1‑143 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CAGCGTATTGATTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCC  >  NC_000913/76667‑76944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑