| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,416,622 | C→G | Q626E (CAG→GAG) | pta → | phosphate acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,416,622 | 0 | C | G | 97.4% | 114.3 / NA | 39 | Q626E (CAG→GAG) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (23/15); total (24/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTG > NC_000913/2416484‑2416757 | ttCGGTATCGAACCGCGCGTTGCTATGATAACATACACAAGCGGTTCTCCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTa > 1:172810/1‑143 (MQ=255) aaCCGCGCGTTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCg < 1:193540/143‑1 (MQ=255) ttGCCATGCTCTCCTCCTCCCCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCACCTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCt < 1:20664/143‑1 (MQ=255) cTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGACGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCCCCGGTCCCCTGCAGTCCGCCCCTGCGCTACAGGACGCCGTTGCGCAAt > 2:623899/1‑143 (MQ=255) cTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGaaag > 1:738188/1‑142 (MQ=255) taaccacgTAATTCTTGTGAAGGAAGCGACGTAGAAAAAGTGCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACTCTGCGGTAATGGCTGACGTTGCGAAATCCaa < 1:9609/136‑1 (MQ=255) tCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCACAGCAACAATGTCCTTTCCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCaa < 1:569528/143‑1 (MQ=255) cACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTACTGGCTGCCGTTGCGAAATCCAAAg > 1:468281/1‑143 (MQ=255) aCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAgc > 1:258256/1‑143 (MQ=255) aCTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGaa < 2:544835/143‑1 (MQ=255) ttCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTACTGGCGGACGTGGCGAAAACCACAGCGACGAAct > 2:70221/1‑143 (MQ=255) cAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCCAAGCGCCGAACTCTCCGGtt > 2:448404/1‑143 (MQ=255) cGACGTAGAAAGCGTTCGCAAAGCAACTCGTCTGGCGCAGGCAAAACGTCCTGACCTGATGGTCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAATTCCAAAGCGCCGAACGCTCCGGTTGCAGGTc < 2:737527/143‑1 (MQ=255) gACGGAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTcg < 2:150127/143‑1 (MQ=255) aCGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTcgc < 1:879355/143‑1 (MQ=255) aaaaaGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCACAGCGCCGAACTCTCCGGGTGCAGGTCGCGCTAcc > 2:165948/1‑143 (MQ=255) aaaaGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGGTGCAGGTCGCGCTACCg > 2:143250/1‑143 (MQ=255) aGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTCCCCAAATCCTAAGCGCCGAACTCTCCGCTTGCAGCTCGCGCTACCgtct > 1:755163/1‑141 (MQ=255) ttCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGGAGGAGGCTGCGGAAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTc > 1:664615/1‑143 (MQ=255) gcgAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCCAAGCGCCGAACTCTCCGGGTGCAGGTCGCGCTACCGTATTCATc > 1:490924/1‑143 (MQ=255) cgAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCt > 1:620814/1‑143 (MQ=255) gAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAGAGCGCCGGCCTCTCCGGTTGCCGGTCGCGCTCTCGTGTTCCTCtt > 1:55723/1‑143 (MQ=255) gAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCCGGTCCCGCTACCGTGTCCATCtt > 1:530659/1‑143 (MQ=255) tCGTCTGGCGGAGGAAACACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCCGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATc < 1:244865/143‑1 (MQ=255) cGTCTGGTGCAGGAAAAACGTCCGGACCTGGTGATCGTCGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGGCGTTGCGAAATCCCAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCt < 2:535096/143‑1 (MQ=255) cGTCTGGCGCAGGAAAAACGTCCTGGCCGTATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCt < 2:468281/143‑1 (MQ=255) cTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCGCCGGTTGCAGGTCGGGCTACCGGGTTCGTCTTCCCGGAtgtgca > 1:84939/1‑141 (MQ=255) gcAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAACTCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGGGCTCCCGAGTTCAACCTCCGGGATCTGAACACCg > 2:410951/1‑143 (MQ=255) aGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACGCCGGt > 2:748694/1‑143 (MQ=255) aGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTACCGGATCTGAACACCGGt < 1:448404/143‑1 (MQ=255) gAAAAACGTCCTGCCCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTaa < 1:497487/143‑1 (MQ=255) gAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCCTCTTCCCGGATCTGAACCCCGGTac > 2:447498/1‑142 (MQ=255) ctgaTGATCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGAGCAATCCAAAGCGCCGAACTCTCGGGTTGCCGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGCACACCGGTAGCCGCACCTatgacgg > 1:10820/1‑137 (MQ=255) tgatgaTCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCg < 1:143250/143‑1 (MQ=255) tgatgaTCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGAGCTACCGTGTTGAGCTTCCGGGAGCTTTACACCGGTGACACCAACTACAAAgag > 2:145884/1‑141 (MQ=255) gatgatCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCCTCTTCCCGGATCTGAACACAGGTAACACCCCCTACCAACCgg > 2:603336/1‑143 (MQ=255) tgatCGACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTa > 1:755714/1‑143 (MQ=255) gACGGTCCGCTGGAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCg > 2:855190/1‑143 (MQ=255) tCCGCTGGAGTACGGCGCTTCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGACGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTg < 2:204557/143‑1 (MQ=255) | TTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTG > NC_000913/2416484‑2416757 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |