Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,662,023 T→C I37V (ATT→GTT)  iscR ← DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,662,0230TC97.8% 121.3 / NA 47I37V (ATT→GTT) iscRDNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (23/22);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGC  >  NC_000913/2661884‑2662160
                                                                                                                                           |                                                                                                                                         
aCGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATCACCACCGCCTGGTCCACGTACGCTGGAATCCATACCATTTTGACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTcc                                                                                                                                        <  1:275152/143‑1 (MQ=255)
    cgcTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTCTTACGCCGACGGCAAAACAGTTGCTCCCGCTAAGCACGGGACACTcccccc                                                                                                                                    >  2:381384/1‑140 (MQ=255)
         aTTACTGCGTGAACGGCTATGGTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTc                                                                                                                               <  1:778171/143‑1 (MQ=255)
          ttccTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCCCGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCg                                                                                                                              <  1:32843/140‑1 (MQ=255)
              ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCGCTGACGTTCGGaaa                                                                                                                          >  1:727134/1‑143 (MQ=255)
                  ccAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATc                                                                                                                      >  1:197696/1‑143 (MQ=255)
                   cAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGCCACCAGACCATTTTTACGCAGACGGGAAAACAGATGCTCCAGATAAGCAAGGGAAACTACCTCACGTCAGGAAATATCa                                                                                                                     >  1:358013/1‑143 (MQ=255)
                   cAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCa                                                                                                                     <  1:645816/143‑1 (MQ=255)
                    aaCGGCGCTGCTGCTGGCATCTCTGCCTAACTGATAACCCCCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAg                                                                                                                    <  1:839004/143‑1 (MQ=255)
                    aaCGGCGATGCTGCTTGCATCTTTGCCTATCTGATAACCACCGCGTGGTCCTCGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAg                                                                                                                    <  1:756382/143‑1 (MQ=255)
                        gCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAGCTCCCTGACGTTCGGAAATATCAGCCaa                                                                                                                >  1:16650/1‑143 (MQ=255)
                          gATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGACACTCCCTGACGTTCGGAAATATCCGCCAACg                                                                                                              >  1:398396/1‑143 (MQ=255)
                                gctgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACCg                                                                                                        <  1:721727/143‑1 (MQ=255)
                                    gCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACGGGGcc                                                                                                    >  2:526606/1‑143 (MQ=255)
                                         tttGCGTAACCGATAACCACTGCCTGGTTTACGTACGGTAGAAACTAGTACATATTTACACAGATGCGAAAGCAGTTGTTCCAGATAAGCAAGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCtt                                                                                               <  2:58892/143‑1 (MQ=255)
                                            gCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCag                                                                                            >  1:351941/1‑143 (MQ=255)
                                             ccTAAGAGATAATCACCGCCTGCTCCACGTACGCGGGAAACCAGACCAGTTTTACGCAGACGGGAAAACAGTTGTGCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCaga                                                                                           <  2:427595/143‑1 (MQ=255)
                                             ccTAACGGAGAACCACCGCCTGGTCCAGGTGCGCTGGAAGCCGGACCATTTTGACGCAGACGGGAAAACAGTTGGTCCAGACAAGAAAGGGAAGCTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCaga                                                                                           <  2:151204/143‑1 (MQ=255)
                                              cTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGCTGCTCCAGATAAGAAAGGGAAACTCCCTGACGGTCGGAATCTTCTACGAACGGTACCGGGGCCGCtgccgcg                                                                                          >  2:8792/1‑137 (MQ=255)
                                               tAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGt                                                                                         >  1:46674/1‑143 (MQ=255)
                                                  catctcaccaccGCCTGGTCCCCGGACGCTGGAAACCAGACCATTGTTCCGCCGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAACCTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGa                                                                                      <  2:850904/137‑1 (MQ=255)
                                                   aGATAACCACCGCCTGGTCCGCGTACGCGGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGGTCGAGATAAGAAAGGGAAAGTCCCTGGGGTGCGGGAATATCAGGCACCGGTACCGGGCCCGCTTGACAGGTGAg                                                                                     >  1:734793/1‑143 (MQ=255)
                                                   aGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCAAACGGTACCAGGCCCGCTTCAGAGTTGAg                                                                                     >  1:721781/1‑143 (MQ=255)
                                                         ccaccGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATCTCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAAc                                                                               >  1:703869/1‑143 (MQ=255)
                                                         ccaccGCCTGGTCCACGTACGCCCGAAACCAGACACACGAGACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACTGTACCGGGCCCGCTTCAGAGTTGAGCGCAAc                                                                               <  1:75886/143‑1 (MQ=255)
                                                          caccGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAACTATCAGTGATCGGGAGCGGGCGCGCTTCAGAGTGGGGCGCAACg                                                                              >  1:166438/1‑143 (MQ=255)
                                                          caccGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACg                                                                              >  1:798661/1‑143 (MQ=255)
                                                                 tGGTGCGCGTACGCTCGAGACCGGACCATTTTTACGCCGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACGCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCa                                                                       <  2:408656/143‑1 (MQ=255)
                                                                    cccATGTATGCTGGAAGCCACACCAGCTTCAGACAGACGGGAAATCAGTTGTTCCATATAAGAAAGGGAAGCTCCCTGACGTTCGGAAATATCAGCCAATGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTg                                                                    <  2:680475/142‑1 (MQ=255)
                                                                     ccACGTACGCTAGAAACCAGACCATTTTTACGCAGACGGGAAAACAGATGTTCCAGATAAGAAAGGGAAACTCCCTGACGGTGGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAAAGTTGAGCGCAACACCACGCAATGc                                                                   >  2:519700/1‑143 (MQ=255)
                                                                           aCGCTGGAAACCAGATCATGTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTATAGAGTTGAGCGCAACGTCAAGCATTGCGGTCAc                                                             <  1:384668/143‑1 (MQ=255)
                                                                               tGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCa                                                         >  2:270945/1‑143 (MQ=255)
                                                                                    aCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATGGCGGTCACGGCATAgcg                                                    >  1:470383/1‑143 (MQ=255)
                                                                                      cAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCAAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCCAGCATTGCGGTCACGGCAGCGCGcc                                                  >  2:733981/1‑143 (MQ=255)
                                                                                         aCCAGTTTTACGCAGACGCGAAAACAGTTGTGCCAGATAAGAAAGGGAAACGCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCtt                                               <  1:187557/143‑1 (MQ=255)
                                                                                             tgttgaCGCGGACGGGAAAGCAGTGGTTCCAGATAAGAAAGGGAACCGCGCGGACGTTCGGAAATATCAGGCAACGGTACCGGGGCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGa                                           <  2:612670/138‑1 (MQ=255)
                                                                                                        aCGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTTCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCa                                <  1:171284/143‑1 (MQ=255)
                                                                                                             aaaaCAGCTGTTCCAGAAAAACAGGAGGGATGCGGTGACGTTCTGTAGTGTCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCt                           <  1:565038/143‑1 (MQ=255)
                                                                                                                    ttgttCCAGATAAGAAATGGAAACTCCCTGACGTTCGAAAATAGCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGTGGTCACGGCATAGCGCCCTGTAGATGTCAGGCTCATGTCTTACTTCa                    <  2:793251/143‑1 (MQ=255)
                                                                                                                    ttgttCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTCCTTCa                    >  2:330142/1‑143 (MQ=255)
                                                                                                                      gttCCAGATAAGAAAGGGAAGCTCACTGACGTTCGGAACTATCAGCCCACGGTCCCGGGCCCGCCTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCGTTAGATGTCAGTCTCATGTCTTACTTCAcc                  <  2:16650/143‑1 (MQ=255)
                                                                                                                       ttCCAGATAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCt                 <  2:703869/143‑1 (MQ=255)
                                                                                                                             aTAAGAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAAcg           >  1:501252/1‑142 (MQ=255)
                                                                                                                                aGAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTccc        >  2:248011/1‑141 (MQ=255)
                                                                                                                                 gAAAGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGcc       <  1:747077/143‑1 (MQ=255)
                                                                                                                                  aaaGGGAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTCCTTCACCTCAAACTCGccg      >  1:328693/1‑142 (MQ=255)
                                                                                                                                      ggAAACTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGCCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTCCTTCACCTCAACCTCGGCCCCGc  >  2:617840/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                         
ACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGC  >  NC_000913/2661884‑2662160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑