Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,398	T→G	L29R (CTT→CGT)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,398	0	T	G	100.0%	92.0 / NA	32	L29R (CTT→CGT)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (12/20);  total (12/20)

TTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAG  >  NC_000913/3041265‑3041524                                                                                                                                      |                                                                                                                               ttttACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCAt                                                                                                                       >  1:196436/1‑143 (MQ=255)    tgccATGAGTCATTAATTCTCCGTTAAGCAACGCAGGACTCTATTATGGCTTTTACTCCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCTCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTAc                                                                                                                    <  2:208422/141‑1 (MQ=255)     tccATTAGTCATTAATACTCCGTTAAGCATCTCAGGACTCTATTATGGCTTTTACCCCTTTTCCTCCCCGTCAGCCTACGGGTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACt                                                                                                                   <  2:332754/142‑1 (MQ=255)               aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGACGACTGGGCGCGTGCCACCCTTACTGGCGCGGACa                                                                                                         >  2:774345/1‑143 (MQ=255)                    tACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGaa                                                                                                    <  1:767235/143‑1 (MQ=255)                    gaCGCCGTTAAGCAACTCAGGACTCTACTATGGCTTTAACCATGTTTCCGCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGaa                                                                                                    <  1:287278/142‑1 (MQ=255)                       gCCGTAAAGCAACACAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGaaaaa                                                                                                 <  1:295954/143‑1 (MQ=255)                         cGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTCCCGCTGACGCTGATGCCGCTTGATGACTGGGCGCGTGCCACCATTACCGGCGCGGGCCGCGacacaca                                                                                               >  2:343531/1‑137 (MQ=255)                          gttgcgCATCTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGGTTACCGCTGACGCTGAGGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAatat                                                                                              <  1:345284/138‑1 (MQ=255)                             aaGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGCAAAATATATg                                                                                           >  1:465249/1‑143 (MQ=255)                                        gACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGt                                                                                <  2:110065/143‑1 (MQ=255)                                        gACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGt                                                                                <  1:774801/143‑1 (MQ=255)                                                tgggggttttACGCGTTTGGCTCCGCGTCAGCCGACGGGTTCTGCGCGTTTACGGCTGACGCGGATGACGCTTGATGACTGGGGGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagcag                                                                        <  1:892261/137‑1 (MQ=255)                                                 atgctttttACTCCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTGTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGa                                                                       <  2:209482/138‑1 (MQ=255)                                                        ctACACCTTCTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGc                                                                <  2:374188/142‑1 (MQ=255)                                                          acacCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa                                                              >  1:644569/1‑143 (MQ=255)                                                              cTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCCTTACTGGCGCGGACAGCGGAAACGATATGCAGGGTCAGGTGGCATCAGGTGTCGGCCGGATg                                                          >  1:276746/1‑143 (MQ=255)                                                               ttttCCTCTTCTTCTGCTTACTGCTGCTGCCCGTTTACCGGTGACGCGGAGGACGCTTGATGACGGGGCGCGGGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATgg                                                         <  1:852979/143‑1 (MQ=255)                                                                 tgcctccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCa                                                       <  2:437089/141‑1 (MQ=255)                                                                     tccTCGTCTGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCGTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaag                                                   <  2:347007/143‑1 (MQ=255)                                                                               ccTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGCGACAGCAGATGTCAGCCAAATGGCAGAAAATCAGCACCt                                         >  2:158700/1‑143 (MQ=255)                                                                               ccTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGGCAGGGGACAGCAGATGTCAGCCAGTTGGCAGAAGATCAGccccc                                         >  2:599232/1‑142 (MQ=255)                                                                                  aCGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCGGAAGATCAACACCTGCt                                      >  1:564034/1‑143 (MQ=255)                                                                                   gtccTTCTGCCCCTTTACCGCTGACGCTGATGACGCTTGAGGACTGGGGGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTc                                     <  2:76228/140‑1 (MQ=255)                                                                                       ttCTGCCCGTTTACCGCTGACGCTGATGACGCTTGCTGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATCTGCAGGGTCAGGTGACACCAGAGAGCAGCCAGATGGCCGCAGATCAGCACCTGCTcgaca                                 >  1:76228/1‑140 (MQ=255)                                                                                                    ccGCTGAAGCTGATCACGCTGGATGACTGGGCGCGTGCCACCATTACTGGCGCGGGCAGCGAAAAATATAGCCATGTTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGc                    <  1:674293/143‑1 (MQ=255)                                                                                                        tgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCaaa                <  2:318612/143‑1 (MQ=255)                                                                                                            gctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGAGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGACGCCCATTGCGACGCCAAAGGTa            >  2:47719/1‑143 (MQ=255)                                                                                                              tgatgaTGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCGAAAGGTaga          >  1:3004/1‑141 (MQ=255)                                                                                                                   ccGCTCGACGACGGCGCGCGGGACACCATCACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtgtg     <  1:4230/142‑1 (MQ=255)                                                                                                                     gCTTCACGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGa   <  1:452013/143‑1 (MQ=255)                                                                                                                      cTTGATGACTGGGCGCGTGCCACCATTAGTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAg  <  2:399672/143‑1 (MQ=255)                                                                                                                                      |                                                                                                                               TTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAG  >  NC_000913/3041265‑3041524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑