Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 87,481 C→A T42K (ACA→AAA)  ilvH → acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091387,4810CA97.0% 80.9 / NA 34T42K (ACA→AAA) ilvHacetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (10/22);  total (12/22)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAGAACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCGGGAAA  >  NC_000913/87344‑87612
                                                                                                                                         |                                                                                                                                   
agagaACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACATCATTGATAGCCTGTCCGTTGCGCCAACCGATGATCCGAAATTAt                                                                                                                                >  1:9676/1‑143 (MQ=255)
 cagaTCCTGTGTATGCGCCGGATATTATCAGTCTTACTCGAGAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTCTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATc                                                                                                                               <  2:627359/142‑1 (MQ=255)
           ttATGCGCCGGATGTTGTGAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTGTTCCCAGCGTGGCTACAACATTGATAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGAcc                                                                                                                     <  2:614688/143‑1 (MQ=255)
           ttATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGAcc                                                                                                                     <  2:493294/143‑1 (MQ=255)
              tGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATc                                                                                                                  <  1:884257/143‑1 (MQ=255)
               gcgtCGGATATTATCAGTCTTTCTCGAAAATGAATCAGGCGCGTGATCCCGCGTGATTGTCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATcc                                                                                                                 <  2:701094/143‑1 (MQ=255)
                               gTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCC‑TTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGGGGGCGGTGaa                                                                                                >  1:558182/1‑143 (MQ=255)
                                      tCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTa                                                                                          >  1:136745/1‑143 (MQ=255)
                                      tCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGAATGCCCACCGAGACCGTGGGCCCTGAAAAAGTa                                                                                          >  2:344406/1‑143 (MQ=255)
                                           aaTGAATCAGGCGCGTTATCCGGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGa                                                                                     <  2:757534/143‑1 (MQ=255)
                                                        cgTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGCCACTCCATCCCGTGGGCGGTGCAAATGTACTTGTGCAGATCGAAAAg                                                                        >  1:754787/1‑143 (MQ=255)
                                                         gTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc                                                                       >  1:814538/1‑143 (MQ=255)
                                                             tCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGCCGATGCAGACCGTGGGCGCAGGAAAAGCACCTGAGCAGAGCGCAAAGCAAta                                                                   >  2:514419/1‑142 (MQ=255)
                                                                    tGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCCGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACaaa                                                            <  1:336340/143‑1 (MQ=255)
                                                                          gcccgttttCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTGGCGCCAACCGACGATCCGAAAGTATCGCGTATGACCATCAAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTc                                                      <  2:626359/138‑1 (MQ=255)
                                                                           ccTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGACCCGACATTATCGCGTCTGACCATCCAGACCGTGGGCGATGAAAAAGGACTTGAACAGATAGAAAAGCAAAAACAAAAAATGGACg                                                     >  1:6552/1‑143 (MQ=255)
                                                                                    ccAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGACCGGCAATTACACAAACTGGTCGgtgtactgc                                            >  2:30233/1‑138 (MQ=255)
                                                                                    ccAGAGTGGCTACAACTTGTCATGAATGACCGTTGCGCCATCCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGTCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTTGTCGATGTCTTgc                                            <  2:355265/143‑1 (MQ=255)
                                                                                        cGTGGCCACAACATGGAAAGCCCGACCGTCGCGCCAACCGACGACCCGAAAGTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCgt                                        <  1:344406/143‑1 (MQ=255)
                                                                                        cGTGCCTACAACATTGACAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCgt                                        <  1:783528/143‑1 (MQ=255)
                                                                                          tcgcgacaacaTTAGTAGCGTGACGGTGGGGCCAACGGACGATCAGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCgtga                                      <  1:393213/138‑1 (MQ=255)
                                                                                             cTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGGAATTATCGCGTGTGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGAGCGAAAAGCACATACCCGATCTGGTCGATGTCTTGCGCctgagtc                                   >  1:378421/1‑142 (MQ=255)
                                                                                              tactacATTGAATGCAGGACCGTGGTGCCAACGGACGGTGCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAGTACACAAACTGGTCGATGTCTTGCGCgtgagtga                                  <  2:870680/143‑1 (MQ=255)
                                                                                              tACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCgtgagtga                                  <  2:718557/143‑1 (MQ=255)
                                                                                                 aacaTTGAAAGCCTGACCGGTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGtt                               <  1:735044/143‑1 (MQ=255)
                                                                                                   cagtGACAGCCTGCCCCTGGCGCCAACCGACCATCCGAAAGTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGAAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTgg                             <  1:279338/140‑1 (MQ=255)
                                                                                                        aaaGCCCGACCGTTGCGCCACCCGACGATCCGAAATTATCGCGTATGACCATCCAGCCCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAg                        <  1:314090/143‑1 (MQ=255)
                                                                                                         agtccTGACCGCTGCGCCAGCCGACGATCGGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGACCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAgg                       <  2:904387/140‑1 (MQ=255)
                                                                                                             cTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGgcgc                   <  1:784079/143‑1 (MQ=255)
                                                                                                                   gTTGCGCGCACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGt             <  1:626395/143‑1 (MQ=255)
                                                                                                                   gTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGt             >  1:371006/1‑143 (MQ=255)
                                                                                                                          cAACCGACGATCCGAGATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAACAGCAATTACACAAACTGGTCGATGTCGTGCGCGTGAGTACGTTGGGGCACGGCGCGCAGCTTGAGCgg      >  2:766159/1‑143 (MQ=255)
                                                                                                                           agcccacgaGCCGAAATTGTCGCGTAGGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCggg     <  1:851034/138‑1 (MQ=255)
                                                                                                                              cgacgaTCCGAAATTAGCGCGTATGACCATCCAGACCGTGCGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATCACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCGGGaaa  <  1:443874/143‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                   
AGAGAACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCGGGAAA  >  NC_000913/87344‑87612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑