| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,085 | Δ1 bp | 100% | coding (2069/2664 nt) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,085 | 0 | T | . | 100.0% | 115.5 / NA | 27 | coding (2069/2664 nt) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (19/8); total (19/8) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGC > NC_000913/124958‑125209 | aTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTGCGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGGGAAAAACAAGAGAAGGTTTATTACTACA‑CACTACGCTGAACGac > 2:104391/1‑142 (MQ=255) tCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCCGGAGCGTATGTACGGTGAAAACCAAGGGAACGTCTACTCCTACA‑CACTACGCTGGATGAACACTAc > 1:34426/1‑143 (MQ=255) tATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCCTCCTGCATGACGGTCTGGCGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGCAAACTACCACATGcc > 1:157006/1‑143 (MQ=255) tATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CAGTACGCTGAACGAAAACTACACCATGcc > 1:337431/1‑143 (MQ=255) tCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGACCGTTTCCTACTACA‑CTCTACGCTGAACGAAAACTACCACATGCCgg < 1:347437/143‑1 (MQ=255) ccGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACAAGCCGGcc > 2:235781/1‑142 (MQ=255) ccGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCGGAACGAAAACTACCACATGCCGGCa > 1:298873/1‑143 (MQ=255) tGTATCTCTTCCGACCCGGCTTACGCTTACGCAGTTGCGGTCATCATGCATGACGGTCTGGAGCGTATGTGCGGTGAAAACCAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCg < 2:127345/143‑1 (MQ=255) tatcctgtACGTCCCGGCTTCCGCTTACGAATTTGCTGTCATCATTCATGACGGGCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGa < 2:75745/136‑1 (MQ=255) tATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAACAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAACGCCGGa > 2:384935/1‑143 (MQ=255) ttacgcttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATTCCGGAAGGTGCTGAGGAAGGTAt < 1:78180/143‑1 (MQ=255) ttacgcttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGGTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTag > 1:66715/1‑142 (MQ=255) tGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAACGGTACCTACaa > 2:40523/1‑143 (MQ=255) tGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTAcca > 1:207817/1‑141 (MQ=255) tGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACac > 1:371819/1‑142 (MQ=255) tGTCATCATGCATGACGGTCTGGAGCGTATGTACGGAGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACAAGCAGGCAATGCAGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTaaaa > 2:400063/1‑143 (MQ=255) catgaggcatgACGGTCTGGAGCCTATATACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACt < 2:227305/137‑1 (MQ=255) tcatGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAAAGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAAGTATCACCAAACTCg > 2:150334/1‑143 (MQ=255) catgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGGCGGAAGGTGCTGAGGAAGGGATCGGGAAAGGTATATACAAACCGGAAACt > 1:425408/1‑143 (MQ=255) aCGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAACGGTATCTACAAACTCGAAACTATTg > 2:441769/1‑143 (MQ=255) ggTCTGGAGCGGATGTACGGGCAAAAACGAGGGAACGTTTACTAGTACA‑CACTACGCTGAACGAAAACTACCACGTGCCTGCAATGCTGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGaa < 2:373922/143‑1 (MQ=255) cTGGAGCGGATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTACCTACAAACTCGAAACTATTGAAGGt < 1:323029/143‑1 (MQ=255) ggAGCGTAGGTACGGTGAAAAACAAGAGAACGTTTACGACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAg < 2:129196/143‑1 (MQ=255) cGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCaaa > 1:241684/1‑143 (MQ=255) aCGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGAAGACAGTGCCGGTAGGTGCTGAGGAAGGTATCCGTACAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAg > 2:309081/1‑143 (MQ=255) aaCAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAgctgc > 2:84054/1‑143 (MQ=255) gagaACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGCAAGGTATCCGTAAAGGTATCTACAAACTCGAACCTATTGAAGGCAGCAAAGGTAAAAGTCCGCAGCCCGGc > 2:467252/1‑143 (MQ=255) | ATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGC > NC_000913/124958‑125209 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |