| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,011 | C→T | 19.4% | R71C (CGT→TGT) | thrB → | homoserine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,011 | 0 | C | T | 19.4% | 19.8 / 4.7 | 23 | R71C (CGT→TGT) | thrB | homoserine kinase |
| Reads supporting (aligned to +/- strand): ref base C (6/12); new base T (2/2); total (9/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.91e-02 | |||||||||||
GGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCC > NC_000913/2869‑3150 | ggTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATACGCTGCCGTCAGAACCACGGCCACATATCGTTTCTCCGTGCTTGTAGc > 1:338912/1‑143 (MQ=255) acacCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGCAAATATCGTTTATCAGTGCTGGGAGCGtat > 2:383504/1‑141 (MQ=255) acCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGttttt < 1:187788/143‑1 (MQ=255) cTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGGTAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGGGGTTTTGc > 1:454642/1‑143 (MQ=255) ttGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAg > 1:227597/1‑143 (MQ=255) gATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCCGAGACATTCAGTCTCAACAACCGCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGCACACATCGTTTATCAGTGCTGGGACCCTTCTCGCCCGGa > 2:424475/1‑143 (MQ=255) ctttGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGCCATGCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGt < 2:419594/141‑1 (MQ=255) ggAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAAt > 2:224202/1‑143 (MQ=255) tgtgGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAAGCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAg < 1:116244/143‑1 (MQ=255) cggcAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTGGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGaa > 2:206547/1‑143 (MQ=255) ggcAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTCAGAAAATTCCAGTGGCGATGACCCTGGaga > 2:204173/1‑141 (MQ=255) caacCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCgg < 2:227597/143‑1 (MQ=255) gcGGACTCTGTGCCGAGAAGCTGCCGTCTGGCGTGCGGGAAAATATCGTTTATCAGTGCTGGGAGCGTGTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTa < 2:353121/142‑1 (MQ=255) tttGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGTGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGAAGGGTTCTGGGTTAGGCTCCAg > 2:430861/1‑143 (MQ=255) tttGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGTGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCACACCGTATCGGTCTTAGGCTCCAg > 2:430985/1‑143 (MQ=255) gCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCGGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCgg < 1:305312/143‑1 (MQ=255) cTGCCGTCAGATCCACGGGAAAAGATCGGTTATCAGGGCGGGGAGCGTGGTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCggt < 1:31480/143‑1 (MQ=255) cTGCCCTCAGACCCACGGGACAAGAGCGTTTGTCAGCGGTGGGAGCTTTTTTGCCAGGAGCTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGATACGAAATGGTCTAGCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCggt < 2:209715/143‑1 (MQ=255) aaaTATCGCTTATCACTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAAGTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGa < 1:96095/143‑1 (MQ=255) gtggacAGTGGCGGGAGCGTTTGTGCGAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGAtgaatgaa < 1:204173/138‑1 (MQ=255) cattttagggAGCGGTTTTGCCATGAAGTGGGGAAGCATATTCCAGTGGCGATGGCTGTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTTCAGTGCTTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTg < 2:447153/136‑1 (MQ=255) cAGTGCTGGGAGTGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTg < 1:430985/143‑1 (MQ=255) cAGTGCTGGGAGTGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTg < 1:430861/143‑1 (MQ=255) gAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGcc < 1:224202/143‑1 (MQ=255) | GGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCC > NC_000913/2869‑3150 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |