| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | 100% | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 98.1% | 25.7 / ‑6.8 | 14 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (6/7); total (7/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTG‑CATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAC > NC_000913/2132658‑2132920 | agGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTG‑CATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctc < 1:268752/143‑1 (MQ=255) gTAATCAGCTGATTTTTTTTGTTGGCGTGGGCGAAGAGGTCGGAGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTG‑CATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttc < 1:15139/143‑1 (MQ=255) aGCTGACATTTTTTGCTGGCGCGGCCGAATATGTCTTTCTGCTTGCGCTATTCTTGGACGCACCCCGCTTACTTTTTCGGTGTCGAGGTGATCTGACCTTCTATCAT‑TTTGTTATGCAGGCTCTGCGTCGCCTCTTCTACttt < 2:461175/143‑1 (MQ=255) cTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTG‑CATTTCACCGTGATTGATATGCAGGCGCTGCGTCGCACCTTCTACTTTcc > 2:253868/1‑143 (MQ=255) ggTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTG‑CATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCCCTGCTACTCTGCACGGTTCGCCCTACttgcac > 2:250056/1‑138 (MQ=255) ttGGGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTA‑CATTTGACGATAGTTGTTATGCAGGCTCTGCGTCGCCTGTTCTACTTTCCACGGTTCGGCGTACTGCACCACCATCCGCAGGaa > 1:66286/1‑143 (MQ=255) cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTG‑CATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGt > 1:188375/1‑143 (MQ=255) tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTG‑CATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTATACTTTCCACAGTTCGCCGTACTCAACCACCATCCGCAGGAAGATGTCGTAATc > 1:289807/1‑143 (MQ=255) gggcttttttCGGTGACGTGGTGATCTG‑CATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg < 2:156912/139‑1 (MQ=255) agaATTTTTTCGGTGACGATGTGATCTG‑TATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTGCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg < 1:60759/143‑1 (MQ=255) gACGAGGTGATCTG‑CATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACGATCCGGAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca < 1:89289/143‑1 (MQ=255) gATCTG‑CATTTCACCGTGATTAATATGCAGGCTCTGCGTCGCATCTTCTACTTTCCACGGTTCGCCGTCCTTCACCACCATCCGCAGGAAGAACTACTAATCATCTACCGCTCGCAGTCCGGTATCGAACAAacaccccttaa > 2:52779/1‑136 (MQ=255) cGTGATTGTTATGCAGGCTCTGCGTCGCCTCTTCTACTGTCCACGGTTCGCCGTTCTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg < 2:262311/143‑1 (MQ=255) atatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGAACGATGACGTAATCCTGTGCCGCTTTCCGTTCGGTATCGAACAAACCCTCTCTGACACCCCATACCCAGCTaaccac > 2:341389/1‑139 (MQ=255) | AGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTG‑CATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAC > NC_000913/2132658‑2132920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |