| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 76,805 | T→A | 100% | E165D (GAA→GAT) | sgrR ← | DNA‑binding transcriptional dual regulator SgrR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 76,805 | 0 | T | A | 100.0% | 52.4 / NA | 20 | E165D (GAA→GAT) | sgrR | DNA‑binding transcriptional dual regulator SgrR |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (11/9); total (11/9) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TATTGATTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAG > NC_000913/76672‑76936 | tATTGATTCGTTTTAAGGAGGCGATCACATCGTCCATTTCCACTTCACGACCATGGTGAAACTGGACTCCTGCACGCACAACGAACCGCCAGCCAAGCGGTGAAAGTTGCTGCCAGTCGCGGCCGATGTCGGCATCCAGTccc > 2:349030/1‑143 (MQ=255) tCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGGTGTCTGAATCCAGTTCCCCAtttc > 2:32695/1‑142 (MQ=255) ttttAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGACATTAGATGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCt > 1:214266/1‑143 (MQ=255) gttAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGATACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTc < 2:443141/142‑1 (MQ=255) aaGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGCTCCCCATTTTCCTCAttt > 1:259744/1‑143 (MQ=255) gCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGATGCCAGTGGTGGGCGATGTCTGCATCCAGTACCCCATATTCCGCAGTTATgcg > 2:123709/1‑143 (MQ=255) tCACGACCATGGTGAAAATGGACTCCTGTACGCTTGAAGAGATGGCAGTGGAGCGGTGAAATGTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCg > 1:473067/1‑143 (MQ=255) tCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAAGTGAAGATTGGCCg > 1:249085/1‑143 (MQ=255) tttaCCATGGTGAAAATGGGCGCATGGACGCAAAAAGAAACGCGAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCggg < 1:351193/140‑1 (MQ=255) aaaaTGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGCACTGAAGATTTGCCGGGCGCTATGGGttt > 1:225485/1‑143 (MQ=255) ggAAACGCCAGTGACGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCa < 1:123836/142‑1 (MQ=255) aCGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTa < 1:251905/143‑1 (MQ=255) tggaGCGTTGAAATTTGCTGCCATTGTTGGGCGATGTCTGCATCGAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGAtt < 2:466374/140‑1 (MQ=255) tGAAGCTGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGGAGATGTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGAtt < 2:468396/143‑1 (MQ=255) tGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACg < 1:265635/143‑1 (MQ=255) aaaTGTGCTGCCAGTGGTGGGCGATGTCTGCAGCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGACCTGAAGAGTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGa < 1:457072/143‑1 (MQ=255) aTTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACg > 1:459695/1‑143 (MQ=255) tttGCTGCCAGTGGTGGGCGATGTCTGCATCCATTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGa < 1:289327/143‑1 (MQ=255) gcCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCAATTTCCTCATTCATGCGCGTTAGCGAACTGAAGATTTGCCCGGCGATATGGGGTTCGGACCGGCGCCATGCGTTGCCAGGTAGCAGATTACGCCGCGGACGCtagcac > 1:420210/1‑140 (MQ=255) gCGATGTCTGCATCCAGTTCCCCATTTTCCGCATTTATGCGCGCTAGCGAACTGAAGATTTGCCGAGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGAGTACGCAACGGCGGAGAGTACAGCCCGCGGAg > 2:126001/1‑143 (MQ=255) | TATTGATTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAG > NC_000913/76672‑76936 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |