| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,398 | T→G | 100% | L29R (CTT→CGT) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,398 | 0 | T | G | 95.0% | 54.9 / ‑3.6 | 20 | L29R (CTT→CGT) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (13/6); minor base A (0/1); total (13/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.91e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAA > NC_000913/3041270‑3041527 | ccATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACATCTTTTCCTTCCCTTTAGCCTTCGGCTTCTGACAGGTGACCGCTGAAGCTGAGGACGCTTGATGACTGGGCGCGTGCCACCATTACTg < 1:256468/143‑1 (MQ=255) tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCGTTACTGGCGCGGa > 2:237260/1‑143 (MQ=255) tCATTAAGACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGa < 1:363346/143‑1 (MQ=255) aCTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGCCAGCGAAAAATATATGCAggg > 1:297256/1‑143 (MQ=255) ggACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAgg < 1:226798/143‑1 (MQ=255) tctATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCCTTACTGGCGCGGACAGCGAAAAATATATGCAGGGGCAGGGGAc > 1:418443/1‑143 (MQ=255) tattatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAGTATATGCCGGGTCGGGTGAcac > 1:464968/1‑142 (MQ=255) tattatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGCGCCACCATTACTGGCGCGGACAGCGAACAATATATGCAGGGTCAGGGGAcag > 2:295679/1‑143 (MQ=255) ttttACACCTGTGCCTCCCCGTCAGCCTACGGCTTCTGCCCGTGTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCa < 1:405051/143‑1 (MQ=255) ttACACCTTTCCCGCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGc < 1:220083/143‑1 (MQ=255) cccGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGGAACATATATGCAGGGGCAGGTGACAGGAGATGTCACCCAGATAGCagaagag > 2:16806/1‑142 (MQ=255) tGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCATGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGccc < 1:288097/143‑1 (MQ=255) gCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCa > 2:363988/1‑143 (MQ=255) cgctgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTAGTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGcc > 1:130518/1‑143 (MQ=255) gctgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGCCAGCCGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCACGCCGCCCATTGCGACGCCa > 1:11230/1‑143 (MQ=255) gctgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGCGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACACCa > 1:236171/1‑143 (MQ=255) cggatgaCGCTTGACGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa < 1:79400/141‑1 (MQ=255) tgatgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCCAACGTaaa > 2:446390/1‑143 (MQ=255) ttGATGACTGGGCGCGTGCCACCCTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAACATGTGGAGc > 1:327802/1‑143 (MQ=255) gatgaCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGAAGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGCAAAATGTGGAGCaa > 2:163005/1‑143 (MQ=255) | CCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAA > NC_000913/3041270‑3041527 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |