| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,176,209 | C→A | 100% | D209Y (GAT→TAT) | cpdA ← | cAMP phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,176,209 | 0 | C | A | 99.6% | 67.0 / ‑7.2 | 26 | D209Y (GAT→TAT) | cpdA | cAMP phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (16/9); minor base G (0/1); total (16/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATG > NC_000913/3176077‑3176341 | cgcgaTCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCGGCGTAAAGTTCCGACAGCGCCGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCtga < 1:239933/139‑1 (MQ=255) cgcATCCGCATGTAGCGCGCGCTGACGCCAGCCGGGCGCGCGGGTATGCAGCGTAACGTCGTAACAGTGCGGCTTAAGCTGCACACAGGTCGACGTCGTTGCCAGTAGGCGGCGACCATTCCAGTCGAGATACAGCTCCtgat < 2:334696/141‑1 (MQ=255) cATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGCCGAGATACAGCTCCTGATGAATATGa < 1:223009/143‑1 (MQ=255) ggaaatCGAGTGTACGCCAGCCGGGGGCGATGGTATACAGGGTATAGCGGGAGCAGTGCGGCTTAAACTGCCCCAAGGACGACGGCGTTGCCAGCAGGCGGGGACCATTCCAGTCGAGAGGCAGCTACTGATGAATATGACCg < 2:468327/138‑1 (MQ=255) gTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCACCTGACGACTATGACCg > 2:246893/1‑143 (MQ=255) ccGAGAGTAGGCCAGCCGGGCGCGATGGGATCCAGCGTAAAGTTGGAACAGTGCGGCTGAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAg < 1:150591/142‑1 (MQ=255) agagTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCaa > 1:139283/1‑143 (MQ=255) gCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGCGATACACCTCCTGTTTAATATAACCGAACACCAACTActta > 1:22333/1‑142 (MQ=255) gCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGGCGACGGCGTCGCCAGCAGGCGGCGACCACTCCAGTCGAGCTACAGCTCCCGATGCATATGACCGCACAGGAGGTAGCTGACgc > 2:303040/1‑142 (MQ=255) gggTATCCAGAGTAAAGGTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATGCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTc < 1:259315/142‑1 (MQ=255) cGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATCTGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACgg > 2:338494/1‑143 (MQ=255) cGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGAGTTGCCAGCAGGCGGCGACCAGTCCAGCCGAGATACAGCTCCTGATCGATATGACCGCACAGCCAGTAATTGACGTGCGAGAACTTCGccggagcga > 2:381671/1‑136 (MQ=255) tAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATGCCAGTCGAGATACAGCCCCTGATGAATATGACCGCACAGGAAGTATTTGACGTACGGAAAATTCGGCAGCACAGTa > 2:402499/1‑143 (MQ=255) tAAAGTTGGAACAGTGCGGCTTAAACGGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTa < 1:432345/143‑1 (MQ=255) caGTTGGAACAGTGCCGCTTAACCTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCACTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATc < 1:270998/142‑1 (MQ=255) aaGTGGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATc < 1:140149/143‑1 (MQ=255) ttGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGGTACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCCCGGCAGCCAg > 2:164553/1‑143 (MQ=255) cGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTCTCCAGTTCGCCCGCGt > 1:281495/1‑143 (MQ=255) acaGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGCCATGCAGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCTAACTGTGtt > 2:136399/1‑143 (MQ=255) cGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGa < 1:472222/143‑1 (MQ=255) gTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCACGTATTTGACGTGCGGAAACTTCGCCAGCTCGGTATACAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACt > 1:392252/1‑143 (MQ=255) gCCAGCAGGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACa > 1:460764/1‑143 (MQ=255) cagcagGCGGCGACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGCTACGCAGACTGTGTTGCTCGAGCCAACTACAAc > 1:134363/1‑143 (MQ=255) ggcgACCATTCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGGGCAAACTACACCCCGCAggg > 1:277539/1‑142 (MQ=255) ttCCAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGgatg > 1:455816/1‑143 (MQ=255) cAGTCGAGATACAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCCACTACAACCCGCAGGTAGCGgatgatg > 1:5969/1‑143 (MQ=255) | TGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATG > NC_000913/3176077‑3176341 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |