| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 87,481 | C→A | 100% | T42K (ACA→AAA) | ilvH → | acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 87,481 | 0 | C | A | 100.0% | 28.4 / NA | 12 | T42K (ACA→AAA) | ilvH | acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (7/5); total (7/5) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGA > NC_000913/87357‑87604 | aTGCGCTGGATATTATCAGTCTTACTCGAAAATTAATCAGGCGCGTTATCCCGCGTGATTTGTC‑TTTTTCTTAGTGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACGGACGATCCGAAATTACCGCGTATGACCATc < 1:430403/143‑1 (MQ=255) cgctattatCAGTCGTACTCCAACATGAATCACGCGCGTTATCCCGCGTGATTGGCCTTTGTTCCCAGCGTGGCTTCAACATTGAAAGCGTGCCCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGAcc < 2:414773/140‑1 (MQ=255) ttACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGCAATTATCGCGTCTGACCATCCCGACGGTGGGCGCTGaaac > 1:164858/1‑142 (MQ=255) aCTCGATAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGGCGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAg < 2:277249/143‑1 (MQ=255) aaaaTGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATAGCGTATGAACATCCAGAACGTGGGCGATGAAAAAGTACtt > 2:153367/1‑143 (MQ=255) cgcgTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTaca > 1:430027/1‑143 (MQ=255) gTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGAGGTCTTGCGCgtg > 1:463102/1‑143 (MQ=255) tGGCTACAACAGTGAGAGCCTGACCTGTGCGCCAACCGACGCTCCGAAATTATCGCGTATGACCATCCAGACCGTGGGTGATGAAAAAGTACTTGTGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCgtga < 2:451086/143‑1 (MQ=255) tACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCgtgagtga > 2:218803/1‑143 (MQ=255) caacaTTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTgagtgagt > 1:102039/1‑143 (MQ=255) tGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAACCTGGTCGATGTCTGGCGCGTGAGTGGGTTGGGGc > 1:207046/1‑143 (MQ=255) gcgcCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGa < 1:316752/143‑1 (MQ=255) | ATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGA > NC_000913/87357‑87604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |