| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,159,068 | G→A | 30.8% | M193I (ATG→ATA) | oxyR → | DNA‑binding transcriptional dual regulator OxyR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,159,068 | 0 | G | A | 30.8% | 27.9 / 15.1 | 29 | M193I (ATG→ATA) | oxyR | DNA‑binding transcriptional dual regulator OxyR |
| Reads supporting (aligned to +/- strand): ref base G (7/13); new base A (7/2); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.02e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01 | |||||||||||
GCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGC > NC_000913/4158928‑4159210 | gcgcTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCt < 2:289940/143‑1 (MQ=255) tttctaaGAATTCATTGAAGAGCAGGGGAATGAAGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACt < 1:42498/138‑1 (MQ=255) cagtcattGGAGTGCCGGTGTTTGATGAGCCAATGTTGCTGGCTATCGATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATACTGGAAGATGGTCACTGTTTgcgc < 1:425880/140‑1 (MQ=255) aaGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGACCGATCTGGCAGGGGAAAAACTGCTGATACTGGAAGATGGTCACTATTTGCGCGATCAGGCa > 1:14752/1‑143 (MQ=255) tGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGTTCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGCTTGCGCGATCAGGCAATg > 2:353821/1‑143 (MQ=255) cggggttCGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGTAAGATGGTTATTGTTTGCGCGATCAGGCAATGGGt < 1:90311/139‑1 (MQ=255) cGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAGACTGCTGATACTGGAAGATGGTCACGATGAGCGCGGTCAGGCAATggga > 2:68663/1‑142 (MQ=255) tttGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTg > 2:45605/1‑143 (MQ=255) catgcGCAAAGGTGGCCGGGTATCTAGGAAGATCACCCGCGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATACTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGttt < 1:271955/142‑1 (MQ=255) cgctcggTTGCCGGCTAGCTACGCAGATCACCCGTGGGCGCACCGCGAATGCGTACCGATGGCCGATCCCGCAGGGGAAACACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAg < 2:178513/137‑1 (MQ=255) ccAATGTGGCGGGGTATCGATGAAGATCAGCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGc < 1:394092/143‑1 (MQ=255) cTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGCTGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCggg > 1:115442/1‑142 (MQ=255) gtcgatGAGGACCACCCGTGGCCGAACCGCGAAGGCGTACCGATGGCCGGTCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGa < 2:247796/139‑1 (MQ=255) atctagGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGa < 1:317102/143‑1 (MQ=255) atGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATACTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATaca > 1:42719/1‑143 (MQ=255) gAATATCACGCGTGGGCGAACCGCGAATGCGTACCGATGGCCTTTCTGGCAGTGTAATTTTTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacaca < 1:17761/143‑1 (MQ=255) aaGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGCAAAACTGCTGATACTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacacac > 2:107818/1‑143 (MQ=255) aTCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATACTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTc > 1:394658/1‑143 (MQ=255) tCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTcc > 2:260581/1‑143 (MQ=255) gTGGGCGAAACGCGAATGCGTACCGATGGCCGATCTGGCAGCGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGa < 1:40302/143‑1 (MQ=255) aaCCGCGAATGCTTACCGATGGCCGATCTGGCAGGGCAAAAACTGCTGATGCTGGAGGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCt < 2:212559/143‑1 (MQ=255) aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGCAAAACTGCTGACGCTGGCAGATGGTCACTGTTTGCGCGCTCAGGCAACGGGCGTCTGTCTTCAAGCCGGCGCGGATGAAGACACCCACCTCCGCCCGACCCGCCTg > 2:77006/1‑143 (MQ=255) cgAATGCGTACCGATGGGCGATCTGGCAGGGGAAACAATGCTGATGCGATAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGaaa < 2:457554/143‑1 (MQ=255) cgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGaaa < 1:45605/143‑1 (MQ=255) cgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGGCACTGTTTGCGCGATCAGGCAATGGGTATCTGTTTTGAAGCCGGTGCGGATGAAGATACACACTTCCGCACGACCAGCCGGGaaa > 2:29686/1‑143 (MQ=255) ccGACGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGACGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCaa < 1:397406/143‑1 (MQ=255) gATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCCGCGCTACAtgttgcc > 1:183313/1‑141 (MQ=255) aGGGGAAAAACTGCTGATACTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTa > 1:331106/1‑143 (MQ=255) aaaaaCTGCTGATACTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACGAGCCTGGAAACTATGCGCAACATGGTGGCGGCAAGTAGAggg > 2:125614/1‑143 (MQ=255) gCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAGCCCTTTACTGc > 1:242824/1‑143 (MQ=255) | GCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGC > NC_000913/4158928‑4159210 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |