Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,472,434 G→A 8.0% R34* (CGA→TGA)  pyrL ← pyrBI operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,472,4340GA 8.0% 123.7 / 2.9 51R34* (CGA→TGA) pyrLpyrBI operon leader peptide
Reads supporting (aligned to +/- strand):  ref base G (22/23);  new base A (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.09e-01

TTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGA  >  NC_000913/4472295‑4472576
                                                                                                                                           |                                                                                                                                              
gtggcagtCAGTTGCGCCGCTGACGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGAtt                                                                                                                                             <  1:375786/136‑1 (MQ=255)
   gCTTTCAGTATCCCCGCTGTCGCCAGCACCCGATTCAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCTTGGGC‑AAAAAAAGCCCCTCGATTGAgg                                                                                                                                         <  1:445475/143‑1 (MQ=255)
     tttCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGCggg                                                                                                                                        >  2:447113/1‑143 (MQ=255)
      ttCGGTGTCGCCGCTGGCGCCAGCACCAGATTAAGGTCAGCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCCCGATTGAgggg                                                                                                                                       <  1:435673/143‑1 (MQ=255)
        cAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCt                                                                                                                                     >  1:341598/1‑143 (MQ=255)
             tCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGaa                                                                                                                                >  1:60247/1‑143 (MQ=255)
               gccgcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCAATCGAGGGGCtttttttt                                                                                                                              <  1:460774/143‑8 (MQ=255)
               gccgcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCAATCGAGGGGCtttttttt                                                                                                                              <  1:361366/143‑8 (MQ=255)
                ccgcGGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATgg                                                                                                                             <  1:112448/143‑1 (MQ=255)
                    tttCGGCAGCACGAGATGGAGGTCATCGCGACTAAGGTCGGTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGa                                                                                                                         <  1:114080/141‑1 (MQ=255)
                      tCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAACGAGCCCCTCGTTTGAGGGGCTGGGAATGGGTGAtg                                                                                                                       >  1:242495/1‑142 (MQ=255)
                        gCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCaa                                                                                                                     <  1:275300/143‑1 (MQ=255)
                        gCCACCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCaa                                                                                                                     <  1:164010/143‑1 (MQ=255)
                          cAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACg                                                                                                                   >  1:452161/1‑143 (MQ=255)
                               ccAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCTCAAAACAGCCCCTCGATTGAAGAACAGGAAATGGGTGATCAACGGgaag                                                                                                              >  1:5195/1‑143 (MQ=255)
                               ccAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCGTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGgaag                                                                                                              >  1:368336/1‑143 (MQ=255)
                                 aGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGgaagaa                                                                                                            >  1:204480/1‑143 (MQ=255)
                                    ttAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGAGATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGaaaaa                                                                                                         <  1:274773/143‑1 (MQ=255)
                                      aaGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACg                                                                                                       <  1:227385/143‑1 (MQ=255)
                                           cATCGCGACTAAAGGCGTTTATGGAAATGATATGTTTATGATATAGCGGATTAGACATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAgg                                                                                                  <  2:163025/143‑1 (MQ=255)
                                              cgcgACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGACTGGGTGATCAACGGGAAGAAAAACGGCAGGCCa                                                                                               >  2:455137/1‑143 (MQ=255)
                                               gcgACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGAATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGACTGAGGGGCTGGGCATGGATGATAAAAGGGAAGAAAAAAGGAAGGCCAg                                                                                              >  2:384348/1‑143 (MQ=255)
                                                        gTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCttttt                                                                                     >  1:451876/1‑143 (MQ=255)
                                                          cGTTTATGGAAATGATATGTTTATGATATAGCGGATTAGCCATCTTTTAGCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTc                                                                                   <  1:261169/143‑1 (MQ=255)
                                                                   aaaTCATTTGTTTCTGATATAGCGGATCATCTATCTTTTATTTCCTGACGCCTTGGCAAAAAAAAGCCCCTCGTTTGAGGGGCTCGGAATGGGTGACCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGt                                                                          <  2:369370/143‑1 (MQ=255)
                                                                             tttCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGACCAACGGGAAGAAAAACGGCAGGCCCGCGTCTTTTTTCACACGCGGCAAGACaaaac                                                                >  2:303373/1‑142 (MQ=255)
                                                                                cTGATATAGCGGATTAGCCATCTTTTATCGCCGGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTc                                                             <  1:243837/143‑1 (MQ=255)
                                                                                 tGATATAGCCGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCg                                                            <  1:204366/143‑1 (MQ=255)
                                                                                   atatAGAGGAAGAGTCACCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGaa                                                          <  2:161265/143‑1 (MQ=255)
                                                                                         cGGATTAGCCATCTTTTATCTCCTGACGCCTGGGTAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACt                                                    >  2:56797/1‑143 (MQ=255)
                                                                                            aTTATCTATCTTATATCTCCGGAAGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAGGAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGaa                                                 <  1:100343/143‑1 (MQ=255)
                                                                                               aacgaTCTTTTATCTCCTCACGCATCGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCa                                              <  1:402533/139‑1 (MQ=255)
                                                                                               aGCCATCTTTGATCAGTCGAGGCGTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCa                                              <  1:62318/143‑1 (MQ=255)
                                                                                                 ccATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCCGACGCGGTAAGACAAACAGTCGAACACACTGAACCATa                                            >  2:112272/1‑143 (MQ=255)
                                                                                                 ccATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATa                                            >  2:398844/1‑143 (MQ=255)
                                                                                                 ccATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATa                                            >  2:362571/1‑143 (MQ=255)
                                                                                                      ttttATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACAtcc                                       >  1:99254/1‑143 (MQ=255)
                                                                                                        gtATCTCATGACGCCTGTGCAAAAAGAAGCCCGTCGATTGGGTGGCTGGGAATGTGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACctcctc                                     <  2:392434/142‑1 (MQ=255)
                                                                                                                          gCAAAACAAAGCCCCTCTGTTGCGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCa                   <  2:427320/143‑1 (MQ=255)
                                                                                                                          gCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCa                   <  1:56797/143‑1 (MQ=255)
                                                                                                                          gCAAAAAAAAGCCCCTCGATTGAGGGGATGGGAAGGGGAGAACAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAACTTGTCCGGCa                   >  2:258933/1‑143 (MQ=255)
                                                                                                                            cattcccaGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCGCGGCAAATTGTCCGGCAtt                 >  2:9065/8‑143 (MQ=255)
                                                                                                                            aagaacaaGCCCCTTTATTGAGGGGCTGGGAATGGGTGATCAAGTTAAAGAAAAACGGCAGTCCAGCTGCTGTCTTAACCCGATGTAATACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCAtt                 <  2:78645/137‑1 (MQ=255)
                                                                                                                              aaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGCTCAACGGAAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATATCGAACACACTGAACCATACATCCTCCCGGCAAATTATCTGGCATtaa               >  2:33401/1‑142 (MQ=255)
                                                                                                                              aaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATCCATCCTCCCGGCACGTTGTCCGGCATtat               >  1:193685/1‑143 (MQ=255)
                                                                                                                                 ccaGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGCCCGGCATTATACt            >  2:236672/3‑143 (MQ=255)
                                                                                                                                   aGCCCGTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCa          <  2:412421/143‑1 (MQ=255)
                                                                                                                                     ccccTCGATTGAGGGGCTGGGAGTGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATCATACTCATc        <  2:452161/143‑1 (MQ=255)
                                                                                                                                      cccTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGACAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCg       >  2:170240/1‑143 (MQ=255)
                                                                                                                                       ccTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACACAGTGTAGAACACACCGAACCATACATCCTCCCGGCAAATTGTCCGGCATTCTACTCATCGt      >  1:342836/1‑143 (MQ=255)
                                                                                                                                         tCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCa    <  2:204480/143‑1 (MQ=255)
                                                                                                                                           gATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCACATTGTCCGGCATTATACTCATCGTCAGa  >  1:219248/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                              
TTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGA  >  NC_000913/4472295‑4472576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑