Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,472,437 T→C 9.3% N33D (AAT→GAT)  pyrL ← pyrBI operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,472,4370TC 9.3% 118.4 / 3.6 52N33D (AAT→GAT) pyrLpyrBI operon leader peptide
Reads supporting (aligned to +/- strand):  ref base T (23/24);  new base C (3/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-02

TTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAA  >  NC_000913/4472295‑4472577
                                                                                                                                              |                                                                                                                                            
gtggcagtCAGTTGCGCCGCTGACGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGAtt                                                                                                                                              <  1:375786/136‑1 (MQ=255)
   gCTTTCAGTATCCCCGCTGTCGCCAGCACCCGATTCAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCTTGGGC‑AAAAAAAGCCCCTCGATTGAgg                                                                                                                                          <  1:445475/143‑1 (MQ=255)
     tttCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGCggg                                                                                                                                         >  2:447113/1‑143 (MQ=255)
      ttCGGTGTCGCCGCTGGCGCCAGCACCAGATTAAGGTCAGCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCCCGATTGAgggg                                                                                                                                        <  1:435673/143‑1 (MQ=255)
        cAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCt                                                                                                                                      >  1:341598/1‑143 (MQ=255)
             tCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGaa                                                                                                                                 >  1:60247/1‑143 (MQ=255)
               gccgcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCAATCGAGGGGCtttttttt                                                                                                                               <  1:460774/143‑8 (MQ=255)
               gccgcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCAATCGAGGGGCtttttttt                                                                                                                               <  1:361366/143‑8 (MQ=255)
                ccgcGGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATgg                                                                                                                              <  1:112448/143‑1 (MQ=255)
                    tttCGGCAGCACGAGATGGAGGTCATCGCGACTAAGGTCGGTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGa                                                                                                                          <  1:114080/141‑1 (MQ=255)
                      tCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAACGAGCCCCTCGTTTGAGGGGCTGGGAATGGGTGAtg                                                                                                                        >  1:242495/1‑142 (MQ=255)
                        gCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCaa                                                                                                                      <  1:275300/143‑1 (MQ=255)
                        gCCACCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCaa                                                                                                                      <  1:164010/143‑1 (MQ=255)
                          cAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACg                                                                                                                    >  1:452161/1‑143 (MQ=255)
                               ccAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCTCAAAACAGCCCCTCGATTGAAGAACAGGAAATGGGTGATCAACGGgaag                                                                                                               >  1:5195/1‑143 (MQ=255)
                               ccAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCGTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGgaag                                                                                                               >  1:368336/1‑143 (MQ=255)
                                 aGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGgaagaa                                                                                                             >  1:204480/1‑143 (MQ=255)
                                    ttAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGAGATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGaaaaa                                                                                                          <  1:274773/143‑1 (MQ=255)
                                      aaGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACg                                                                                                        <  1:227385/143‑1 (MQ=255)
                                           cATCGCGACTAAAGGCGTTTATGGAAATGATATGTTTATGATATAGCGGATTAGACATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAgg                                                                                                   <  2:163025/143‑1 (MQ=255)
                                              cgcgACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGACTGGGTGATCAACGGGAAGAAAAACGGCAGGCCa                                                                                                >  2:455137/1‑143 (MQ=255)
                                               gcgACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGAATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGACTGAGGGGCTGGGCATGGATGATAAAAGGGAAGAAAAAAGGAAGGCCAg                                                                                               >  2:384348/1‑143 (MQ=255)
                                                        gTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCttttt                                                                                      >  1:451876/1‑143 (MQ=255)
                                                          cGTTTATGGAAATGATATGTTTATGATATAGCGGATTAGCCATCTTTTAGCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTc                                                                                    <  1:261169/143‑1 (MQ=255)
                                                                   aaaTCATTTGTTTCTGATATAGCGGATCATCTATCTTTTATTTCCTGACGCCTTGGCAAAAAAAAGCCCCTCGTTTGAGGGGCTCGGAATGGGTGACCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGt                                                                           <  2:369370/143‑1 (MQ=255)
                                                                             tttCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGACCAACGGGAAGAAAAACGGCAGGCCCGCGTCTTTTTTCACACGCGGCAAGACaaaac                                                                 >  2:303373/1‑142 (MQ=255)
                                                                                cTGATATAGCGGATTAGCCATCTTTTATCGCCGGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTc                                                              <  1:243837/143‑1 (MQ=255)
                                                                                 tGATATAGCCGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCg                                                             <  1:204366/143‑1 (MQ=255)
                                                                                   atatAGAGGAAGAGTCACCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGaa                                                           <  2:161265/143‑1 (MQ=255)
                                                                                         cGGATTAGCCATCTTTTATCTCCTGACGCCTGGGTAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACt                                                     >  2:56797/1‑143 (MQ=255)
                                                                                            aTTATCTATCTTATATCTCCGGAAGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAGGAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGaa                                                  <  1:100343/143‑1 (MQ=255)
                                                                                               aacgaTCTTTTATCTCCTCACGCATCGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCa                                               <  1:402533/139‑1 (MQ=255)
                                                                                               aGCCATCTTTGATCAGTCGAGGCGTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCa                                               <  1:62318/143‑1 (MQ=255)
                                                                                                 ccATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCCGACGCGGTAAGACAAACAGTCGAACACACTGAACCATa                                             >  2:112272/1‑143 (MQ=255)
                                                                                                 ccATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATa                                             >  2:398844/1‑143 (MQ=255)
                                                                                                 ccATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATa                                             >  2:362571/1‑143 (MQ=255)
                                                                                                      ttttATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACAtcc                                        >  1:99254/1‑143 (MQ=255)
                                                                                                        gtATCTCATGACGCCTGTGCAAAAAGAAGCCCGTCGATTGGGTGGCTGGGAATGTGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACctcctc                                      <  2:392434/142‑1 (MQ=255)
                                                                                                                          gCAAAACAAAGCCCCTCTGTTGCGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCa                    <  2:427320/143‑1 (MQ=255)
                                                                                                                          gCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCa                    <  1:56797/143‑1 (MQ=255)
                                                                                                                          gCAAAAAAAAGCCCCTCGATTGAGGGGATGGGAAGGGGAGAACAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAACTTGTCCGGCa                    >  2:258933/1‑143 (MQ=255)
                                                                                                                            cattcccaGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCGCGGCAAATTGTCCGGCAtt                  >  2:9065/8‑143 (MQ=255)
                                                                                                                            aagaacaaGCCCCTTTATTGAGGGGCTGGGAATGGGTGATCAAGTTAAAGAAAAACGGCAGTCCAGCTGCTGTCTTAACCCGATGTAATACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCAtt                  <  2:78645/137‑1 (MQ=255)
                                                                                                                              aaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGCTCAACGGAAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATATCGAACACACTGAACCATACATCCTCCCGGCAAATTATCTGGCATtaa                >  2:33401/1‑142 (MQ=255)
                                                                                                                              aaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATCCATCCTCCCGGCACGTTGTCCGGCATtat                >  1:193685/1‑143 (MQ=255)
                                                                                                                                 ccaGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGCCCGGCATTATACt             >  2:236672/3‑143 (MQ=255)
                                                                                                                                   aGCCCGTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCa           <  2:412421/143‑1 (MQ=255)
                                                                                                                                     ccccTCGATTGAGGGGCTGGGAGTGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATCATACTCATc         <  2:452161/143‑1 (MQ=255)
                                                                                                                                      cccTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGACAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCg        >  2:170240/1‑143 (MQ=255)
                                                                                                                                       ccTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACACAGTGTAGAACACACCGAACCATACATCCTCCCGGCAAATTGTCCGGCATTCTACTCATCGt       >  1:342836/1‑143 (MQ=255)
                                                                                                                                         tCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCa     <  2:204480/143‑1 (MQ=255)
                                                                                                                                           gATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCACATTGTCCGGCATTATACTCATCGTCAGa   >  1:219248/1‑143 (MQ=255)
                                                                                                                                            aTTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGaa  >  1:395540/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                            
TTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAA  >  NC_000913/4472295‑4472577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑