| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 125,085 | Δ1 bp | coding (2069/2664 nt) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,085 | 0 | T | . | 93.5% | 111.0 / 5.9 | 33 | coding (2069/2664 nt) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base . (16/13); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTT > NC_000913/124964‑125216 | tCGGTGACTATCCAGAACTGTATCTTTTAAGACTCGGCTTACGTTTATGAAGTTGCTGTTATCATGTATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTAc < 1:21871/143‑1 (MQ=255) cGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCggaca > 2:267358/1‑140 (MQ=255) cGAACTGTATCTCTTACGACCCGGCTTACGCATACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGCACGTTTACTACTACA‑CACTACGCTGAACGCAAAATAACAGAAGCAGGCaa > 2:322713/1‑143 (MQ=255) aaCTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCTTGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATg < 1:459504/143‑1 (MQ=255) cTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGCACGTATGTACGGTGAAAAACAAGAGAACGCTTACTACTACA‑CAATACGCTGAACGGCCGCTACCAAATGCCCGCGACGcc > 2:49058/1‑143 (MQ=255) tATCTCTTACGACCCGGCTTACTTTTACTAAGTTGCTGTCATCATGCATGACGGTCTGGAGAGTATGTACGGTGAAAAACAAGAGAACGTTGACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGa < 1:801952/143‑1 (MQ=255) tATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGa > 1:651471/1‑143 (MQ=255) aTCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGATCGTATGTACTTTTATTTTCTAGTTAACGTTTACTACTACA‑CACGACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGaa < 1:666795/143‑1 (MQ=255) tctcTTATTACCTGGCTTACGCTGAAGAAGTGGCTGTCAGCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAg < 1:320221/143‑1 (MQ=255) cccGGCTTACGCTTACGAAGTTGCTGTCATCATTCATTACGTTCTTTATTTTATGTACGGTGAAAAACAAGAAAACGTGAACTACAACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGa < 1:26998/143‑1 (MQ=255) gTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGCTCAACAACAAGAGTACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTGAAGGTATc < 2:484505/143‑1 (MQ=255) tGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAACGCAGGAAGGCGCCGAGGGAGGCGTCCGTGAAGGTATCcacac > 2:366152/1‑142 (MQ=255) gTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGAGCAGAGAAAAGTATAAGTAAAGGCAACTACaaa > 1:725238/1‑143 (MQ=255) tcatGCCTGTCGGTCTGGAGCGTATGTACTGTGACATACAAGAGAACGTTGACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCg < 1:567873/143‑1 (MQ=255) tgcatgGCGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTAC‑GCGCTCTGCTGTACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGaaa < 2:236877/143‑1 (MQ=255) tgcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTAC‑TCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGaaa < 1:483033/143‑1 (MQ=255) tgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTTAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTAt < 1:267358/143‑1 (MQ=255) gTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGCAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAg < 1:416013/143‑1 (MQ=255) gTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAg < 2:651471/143‑1 (MQ=255) cGTATTTACGGTGAAATACAAGAGATTGTTTACTTCT‑TTTTTTTTCTCTGAACTAAAACTACCACGAGCAGGCAATGCCGGAAGGTGCGGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCaaa < 1:55036/143‑1 (MQ=255) cGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAACACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGAATCCGTAAAGGTATCTACAAACACGAAACTATTGAAGGTAGCAAAGGTAAAGt > 1:92087/1‑143 (MQ=255) ggTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACACGAAACGATGGAAGGTAGCAAAGGAAAAGtt > 2:245215/1‑143 (MQ=255) ggTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGATGAGAAAGGTATCCGGAAAGGTATCTACAAACTCGAAACTACTGAAGGTAGCAAAAGCAAAGtt > 2:164670/1‑143 (MQ=255) tGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAGACTATTGAAGGACGCAAAGGTAAAGtcaa > 2:331205/1‑140 (MQ=255) aaaaaCAAGAGAACGTTTACTACTACA‑CACTACGCTGCACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATAGAAGGTAGCAAAGGTAAAGTTAAgc > 1:470359/1‑143 (MQ=255) aaaaaCAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGCATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAgc > 1:589834/1‑143 (MQ=255) aaaaaCAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCAAATGCCGGCAATGCCGGAAGGTCCTGAGGAAGATATCCGTAAAGGTATCTACCAACTCGAAACTCTTGCAGGTAGCAAACGGAAAGTTCAgc > 2:800092/1‑143 (MQ=255) aaaaCAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGCGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACAATAGAAGGTAGCAAAGGAAAAGTTAAgct > 1:669342/1‑143 (MQ=255) agagAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCTGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCgg < 2:357735/143‑1 (MQ=255) cGTTTACTACTACA‑CACTACGATGAACGAAAACTACAACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAAATATTGAAGGTAGCACAAGTACAGTTCAGCTGCTCGACTACgg > 2:373839/1‑143 (MQ=255) gTTTCCTACTAC‑CCACTCAGTTGAGCGAAAACTACCACCGGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGt < 2:176019/143‑1 (MQ=255) tttACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGCAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACCAACTCGAAACTAGCGAAGGTAGCAAAGGTAAAGTTCACCTGCTCGGCTCCGGtt > 2:533908/1‑143 (MQ=255) gttACTAATACA‑CATTACGTAGAAAGAAAACTAACACAGGCCGGCAAGGGCGGAAGGGGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGtt < 1:534470/142‑1 (MQ=255) | TCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTT > NC_000913/124964‑125216 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |