| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,560,455 | +G | pseudogene (151/758 nt) | glpR ← | DNA‑binding transcriptional repressor GlpR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 88.9% | 70.0 / 3.0 | 27 | pseudogene (151/758 nt) | glpR | DNA‑binding transcriptional repressor GlpR |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base G (10/14); total (12/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGT > NC_000913/3560317‑3560591 | aTAAACAGCGTCGAGCCATTGGGTATTTGCTCCGCCACTTAGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGTCGCACCGCCATGA‑G‑GG‑cgcaa > 2:283874/1‑139 (MQ=255) tAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTACGATTCAGTCTGGGACCCCCTGCGTCCGTGCCACGGCGTCTTACCCCAACGGGAAGCCCGCGCAGCACCGGCATCA‑T‑CG‑cgccgg > 2:768886/1‑138 (MQ=255) gAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCTGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGGTAACCGGACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTg < 1:326019/143‑1 (MQ=255) gAGCCATGGGGTGTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTg < 2:634864/143‑1 (MQ=255) aGCCACGGGGGAGTTGCTCCGCCATTTTGCGGGCGATGTGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTGAACCGATCTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGc < 1:115704/143‑1 (MQ=255) gCCATTGGGGATTTTCTCCGCCACTTTGCGGGCGATGCGATCTTTTTCTTCGGTCTGGGTGGCCATGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCt < 1:273625/143‑1 (MQ=255) ttGGGGATTTGCTCTGCCTCTGTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTTGCTTTGTGATCGTGCCACGGCGTGTGAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGc < 1:753947/143‑1 (MQ=255) ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGGAAGGCAGCGCAGCACCGACAAGA‑A‑GGAG‑CAAGAACAGGTTTTGATcaac > 1:616595/1‑140 (MQ=255) gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTGTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATAA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAg > 1:525089/1‑143 (MQ=255) gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTATTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAACGCAGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTGTTGCTCCGCCAg > 1:525081/1‑143 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCTTTTTTTTTTTTTTTGATGGCCTAGCGATCGTGCCACGGCGTGTTAATTGATCTGGGAGGCAGCGCCGCACCGTCCTGACT‑GC‑G‑CAGGATCAGTCTGTGGTCGGCCAGTTCAtttgc > 2:181397/1‑140 (MQ=255) ccACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCCCCAGCTCAGTGAGGTcg > 2:751876/1‑143 (MQ=255) cgttGGGGGCGTTGCGCTGTCTTTGCTCGCTCTGGGTGGTCTTGCGAGCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCTGCACCGCCATGA‑T‑GG‑CGCAGGATCATGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg < 2:669136/141‑1 (MQ=255) tttGCGGGGCATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAGCTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc < 1:322159/143‑1 (MQ=255) tctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCGACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCAGCAAGCTCATTGAAGTCGCAGAGAATAATCTGAGGGCt > 1:526434/1‑143 (MQ=255) cttcGGTCTCGGTGGCCTTGCGATCGTGACACGGCGTGTTAACCGAACAGGAAAGCAGCGCCGCACCCCCATGA‑T‑GG‑CGCAGGATCAGGTGTTGCTCCGCCAGCTCATTGAGGGCGCGGCGAATAGGCTGCGGCCTGACGgac > 2:511751/1‑142 (MQ=255) gTCTGGGTGGCCTGGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg < 1:570843/143‑1 (MQ=255) tGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCGCCGGCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc < 1:275814/143‑1 (MQ=255) cccTTCCGTTCGTTCCACGGCTCGGCAACCGAACTTGAAGGCAGCGCCGCACCGCCACGA‑T‑GG‑CGCAGGATCAGGTTTTGCCCCGCCAGCTCATTGAGGTCGCGGCGTATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg < 2:113947/142‑1 (MQ=255) gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGGCTGCAGGCTGACGGAGAAAAGCTCTACCCGCCCTTCGGTAc > 1:267320/1‑143 (MQ=255) cACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTTCGCCAACTCATTGAGGTCGCGGCGAATAGTCTAAGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTgtc > 1:510625/1‑141 (MQ=255) aCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCCGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACa > 1:774676/1‑143 (MQ=255) gtgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTAGCGGCGAATAGTCTGCGGGATGAACGTAATGCACTCTAACAGCTCTTCGGTACTGACATAAca > 2:161504/1‑142 (MQ=255) gtTAACCGAACTGGAAGGCAGTGCTGCATCGTTATGA‑T‑GT‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAGAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct < 1:534694/143‑1 (MQ=255) ttAACCGTACTGGAAGGCAGCGCCGCACCGCTATGA‑T‑GG‑CGCAGGATCAGGTTTGGCTCCGCGAGCTCAGTGAGGTCGCGGCGAATAGTCGGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg < 1:315354/143‑1 (MQ=255) ttAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg < 1:315347/143‑1 (MQ=255) agcttgAAGGCAGCGCCGGACCGGCATGA‑TGGG‑T‑GTGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCGCTACCAGCTCTTCGGTACTGACATAACGCTGCTGTTTa < 2:45583/138‑1 (MQ=255) gcgcCGCTCCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGata < 2:683400/143‑1 (MQ=255) ga‑t‑gG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGt > 1:577696/1‑143 (MQ=255) | ATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGT > NC_000913/3560317‑3560591 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |