Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,293,032	IS1 (–) +8 bp	intergenic (‑110/‑488)	hns ← / → tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	257908 =	NA (NA)	36 (0.820)	31/284	0.4	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 1293039	0 (0.000)					intergenic (‑117/‑488)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
*	?	NC_000913	1293032 =	0 (0.000)	42 (0.960)	35/284	0.2	100%	intergenic (‑110/‑495)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
	?	NC_000913	= 1979270	NA (NA)					noncoding (1/768 nt)	IS1	repeat region

CCTACGATAATCTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1293171‑1293032 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCG  <  NC_000913/1979270‑1979130                                                                                                                                                                                                                                                                                           CCTACGTTAATCTCCCTATTAAATTTTTCTTTTATCATTTAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGGAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGT                                                                                                                                            <  1:318760/143‑1     CGATGATCTCTCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATG                                                                                                                                        <  2:15191/143‑1            CTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAA                                                                                                                                 <  2:533346/143‑1             TCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATCTGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGAGATGCTGCCAAC                                                                                                                                >  1:310160/1‑143                CCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCAAACTTA                                                                                                                             >  1:450860/1‑143                    AAAATCTGGCACGAATCAGGAATTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATCCTGCCAACTTACTGA                                                                                                                         <  2:698932/143‑1                      AATGTGATATGAATCAGGAAGTTTTAATCTCACGTGTTGCGAAATCATCGGTGTAAATAGGGTTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATT                                                                                                                       <  2:119048/143‑1                          TGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGT                                                                                                                   >  2:799945/1‑143                           GACATGATTCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTG                                                                                                                  <  2:618955/143‑1                                       GAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTATGGCAAATCCTATCAAAAGATACTTCTTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTT                                                                                                      >  2:49125/1‑143                                         AGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTT                                                                                                    <  1:620178/143‑1                                         AGTTGTAACCTCACGTGCTGCGAAATCATTGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTT                                                                                                    <  2:305549/143‑1                                            TTTCACCTCACGTGTTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCTTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGCTGTTTTTGA                                                                                                 <  2:633995/143‑1                                            TTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGTGCTATATGTCGCGTCTTTTCTGGATAATTTTATGAAAAGATATTTATTGTCGGCACAAGGTGATGCTGCCACCTTACTGATTTAGTGTATGATGGTGTTTTTGA                                                                                                 <  1:419858/143‑1                                             TTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAG                                                                                                <  1:77219/143‑1                                             TTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTAAGAGTATAATGGTGTATTTGAG                                                                                                >  1:652144/1‑143                                                accgctCGTGCTGCGAAAGTATCGGTGTAAATAGGGCTAGATGCCGCGTCTTTGCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTG                                                                                             <  2:18467/137‑1                                                 CCTCACGTGCTACCAAATCAGCGTTTTAAAGTGTCTTATATGCCGTGTCTTTTCTGTCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGC                                                                                            <  2:421910/143‑1                                                  CTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTCTGAAAAGCTCTTTATTGGCGGCACAAGGTGATGCTACCAACTTACTGAATAAGAGTATGATGGTGTTTTTGAGGTGCT                                                                                           >  1:318636/1‑143                                                   TCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTC                                                                                          <  1:461269/143‑1                                                          cgGCGAAACCATCGGTGTAAATATGGCTATATGCAGGGTCTGTTCTTGCTAATTTTATGAAAAGAGATTGATTGGCTGCACAAGGTGATGCTGCCAACTTACTGATCTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGC                                                                                   <  2:233414/141‑1                                                                    ATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTCATTTTATGAAACGATATTTATTGGCGTCACAAGGTGATGCTGCCAACTTACTAAAATAGTATAAAAAGATGTTTTTGAGGTGATCCAGTGGCTAATGTTTCT                                                                         >  1:374394/1‑143                                                                      cgcTGTATATAGGGCTATATGCCGCGTCTTTTCTGGCGAACTGGATGAAATGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTAT                                                                       <  2:806667/140‑1                                                                      CGGTGTAAATAGGGCCATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTAT                                                                       <  2:752661/143‑1                                                                       GGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATC                                                                      <  1:799945/143‑1                                                                        GTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCA                                                                     <  1:415418/143‑1                                                                          GTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGC                                                                   >  1:412573/1‑143                                                                           TAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGACGGTGTTTTTGAGGTGCTCCAGTGGCTCCTGTTTCTCTGAGCT                                                                  >  2:76254/1‑143                                                                                  GGCTATATGCCGCGTCTTTTCTGACTAATTTTATGAAAAGATATTTATTTGCGGCACAAGGTGATGCTGCAAACTTACTGAATTAGTATAAGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTC                                                           >  1:107896/1‑143                                                                                      attTGCCTCGTGTTTTCTTGCTAATTTTATGAAAAGATATTTATTGGCTGGACAAGGTGATGTTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGT                                                       <  2:232197/140‑1                                                                                          GCCGCGTCTTTTCTGGCTACTTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAG                                                   <  2:379736/143‑1                                                                                           CCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCCCCTTACTGTTTTAGTGTATGATGGTGTTTTTGAGGTACTACAGTGAATTCTGTTTCTATCAGCTGTCTCTCTGGTTCAGC                                                  >  1:41441/1‑143                                                                                                        GGCTAATTTTATGATAAGTTATTTATTGGCGGCACAAGGTGATGCTGCCAACGTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTG                                     <  1:807749/143‑1                                                                                                        GGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTG                                     <  1:433621/143‑1                                                                                                            AATTTTATGAAAAGATATTTATTGGCGGCATAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGAGGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGC                                 <  1:744665/143‑1                                                                                                                  ATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAATTTACTTATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACG                           <  1:354604/143‑1                                                                                                                   TGAGAAGATATTTATTGGCGGCACAAGGTGATGCTGCCACCTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGt                          <  2:334829/143‑2                                                                                                                      AAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGCGCGTAACGGCAA                       >  2:602670/1‑143                                                                                                                            ATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCAC                 >  1:309785/1‑143                                                                                                                               TATTGGTTGCACTAGGTGATGCTGCCAACTTACTTATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTTTCTTCTGTTTCTATCAGCTGTCCCTCCTGTTTAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGC              <  2:58662/143‑1                                                                                                                                    GCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGATTCTATCAGCAGAACCACCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCGAAGGCACCGCCGGAC         >  1:392087/1‑143                                                                                                                                           AAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCATCTGTCCCTCCTGTTCAGCTACTGACTGGGTTGTGCGTACCGGCAAACGCACCCCCGCACCTCAGCG  >  1:781774/1‑143                                                                                                                                                                                                                                                                                           CCTACGATAATCTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1293171‑1293032 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCG  <  NC_000913/1979270‑1979130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.