Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	76,805	T→A	E165D (GAA→GAT)	sgrR ←	DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	76,805	0	T	A	100.0%	94.4 / NA	37	E165D (GAA→GAT)	sgrR	DNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (23/14);  total (23/14)

TTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGC  >  NC_000913/76678‑76943                                                                                                                                |                                                                                                                                           ttCGTTTTAAAGAGGCGATCACATCGTCGATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCAttt                                                                                                                             <  2:770066/143‑1 (MQ=255) ttCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCAttt                                                                                                                             >  1:39675/1‑143 (MQ=255)     ttttAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGAACTCCCGGAAGCACACAGAAACGCCAGTGAAGCGGAGACATTTGCCGCCAGTGCTGGACGCTGTCTGCATCCAATTCcacactatacg                                                                                                                         >  2:272878/1‑136 (MQ=255)       ttAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCa                                                                                                                       >  2:556473/1‑143 (MQ=255)            gagGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTAt                                                                                                                  <  1:707298/143‑1 (MQ=255)                   tCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCAAGAGGTGGGAGATATCTAAATACAGTTCAACATTTTACTAATTTATGCGCGtt                                                                                                           >  1:74993/1‑143 (MQ=255)                      caTCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGGGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGc                                                                                                        <  1:657846/143‑1 (MQ=255)                            ccATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCAAGTTCCCAATTTTCCTCATTTATGCGCGTTAGCGAACTg                                                                                                  >  1:433740/1‑143 (MQ=255)                              aTTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTCCCAGTGGTGGGCGAAGTCTGCAACAAGTTCACCATTTTCCTCATATATGCGCGTTAGCGAACTGaa                                                                                                >  1:383798/1‑143 (MQ=255)                                   cAGTTCACGACCATGGTGAAAATGGAATCCTGGACGCAAAAAGAAACGCCAGTGAATCGGTGAAATTTGATGCAAGTGGTGGGCGAGGTATGAATCCAGGTCACCATTTTCCTCATTTATGCGCGTTTGCGAACTGAAAAttt                                                                                           >  2:21504/1‑143 (MQ=255)                                       tCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCg                                                                                       >  1:451514/1‑143 (MQ=255)                                         aCGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCggg                                                                                     >  2:704559/1‑143 (MQ=255)                                           gACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCg                                                                                   <  1:704559/143‑1 (MQ=255)                                            aCCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTCGCGCACTGAAGATTTGCCGGGCGa                                                                                  >  2:504557/1‑143 (MQ=255)                                                tgttGAAAATGGCTTCCTGGACGCAAAAAGCATCGCCAGTGAAGCGGTGAAGTTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATg                                                                              <  2:619449/140‑1 (MQ=255)                                                    gAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGACATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGtt                                                                          >  1:416955/1‑143 (MQ=255)                                                    gAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCAATTATGAGCGTTAGCGAACTGAAAATTTGCCGGGCGATATggggt                                                                          >  1:62860/1‑141 (MQ=255)                                                              tCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGgc                                                                >  1:409452/1‑143 (MQ=255)                                                                     cGCAAAAAGATACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTTGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATGTGCCGGGCGATATGGGTTTCGGAACGGCGCAATgc                                                         <  1:661271/143‑1 (MQ=255)                                                                      gCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGCTATGGGTTTCGGAACGGCGCAATgcg                                                        >  2:120193/1‑143 (MQ=255)                                                                        aagaaaaaaCGGCAGTGAAGTAGTTAAATTTGTTTTCAGTGGTGGGCGATGTCTGCATCCATTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGTATGGCGATATGGGTTTCGTAACGGCGCAATGCGCt                                                      <  2:225541/137‑1 (MQ=255)                                                                        aaaaaGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTAGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCCTTTATGCGCGTTAGCGAACTGAAGATTTGCAGGGCGATATGGGTTTCGGAACGGCGCAATccgcg                                                      >  2:201413/1‑142 (MQ=255)                                                                            aGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCa                                                  <  2:39675/143‑1 (MQ=255)                                                                                   ccAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCAATTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTATCGTAACGGCGCAATGAGATGCCAggacgca                                           >  2:104292/1‑138 (MQ=255)                                                                                     aGTGAAGCGTTGACATTTGCTGCCAGGGTTTGGTGATGTCTGCATTCCGTTCCCCTTTTTCCTCATTTATGCGCGTTAGCGAACTGCAGATTTGCCGGGCGATATGGTTTTCGGAACGGCGCAATGCGCTGTCAGGTAGCAGa                                         <  2:699571/143‑1 (MQ=255)                                                                                             ggTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCaa                                 >  1:216674/1‑143 (MQ=255)                                                                                              gTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGATGCCAGGTAGCAGATTACGCAAc                                >  1:481978/1‑143 (MQ=255)                                                                                               tGAAATTTGCTGCCAGGGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACg                               >  1:14100/1‑143 (MQ=255)                                                                                                        ctgttaGTGGTTGGCGATGTCTGCATCCAGTTCCCTATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGAtagt                      <  1:730138/138‑1 (MQ=255)                                                                                                         tgcCAGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGAtagta                     <  2:99376/143‑1 (MQ=255)                                                                                                          gcCAGTGGTGGGCGATTGCTGCATCCAGTTCTCCATTTTCCTCATTTATGCGCGTTTGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGAtagtag                    <  2:62860/143‑1 (MQ=255)                                                                                                             aGTGGTGGGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGc                 >  1:120946/1‑143 (MQ=255)                                                                                                                 gtggGCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCAcgc             >  1:667614/1‑143 (MQ=255)                                                                                                                    ggCGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCa          <  2:703224/143‑1 (MQ=255)                                                                                                                      cGATGTCTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGACAGGTAAAAGAATACGCAACGGACGATAGTAGAGCACGCGAAgg        >  1:783734/1‑143 (MQ=255)                                                                                                                      cGATGTCTGCATCCAGTTCCCCATTCTCCTCATTTATGCGCGTTAGCGAACTGCAGATTTGCCGGGCGATATAGGTTCCGGAACGGCGCAAAGCTCTGCCAGGTAGCAGATTCCGCAACGGAGGATAGCAGAGCACACGCaag        >  2:274891/1‑141 (MQ=255)                                                                                                                            cTGCATCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTgc  <  2:409452/143‑1 (MQ=255)                                                                                                                                |                                                                                                                                           TTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGC  >  NC_000913/76678‑76943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑