BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,247	A→G	L375M (TTA→CTG)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,247	0	A	G	94.7%	34.4 / NA	20	L375M (TTA→CTG)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (1/0); major base G (11/7); minor base T (0/1); total (12/8)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGA  >  NC_000913/1196114‑1196315

aagtcgcCCTGTTTGAAGACACTTACGGTACTGCGCCGAAATATGCCGCTCAGGACAATGTAAATCCTTTCTCTTTTATTCTCTCCGCTGAGATGCTTCTGCGCCATATGGGTTTGACTGAAGCGGTTGATCTGATTGTTaaa                                                             <  2:465127/139‑1 (MQ=255)

aaTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                                             <  1:727946/143‑1 (MQ=38)

    cgcCCGGTGTGGAGCCACCCACGGTACTGCGGCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTa                                                         <  2:593598/143‑1 (MQ=25)

     ggccTGTGTGCAGCCAACCACGGTACAGCGCCGAAATATGCCGGTCAGGGCAAAGTAAATCCTGGCTCTATAATTCTTTCCGCGGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt                                                        <  2:713344/141‑1 (MQ=21)

     gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAACTGATTGTTAAAGGTAt                                                        >  2:339431/1‑143 (MQ=38)

       ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTACACCATATGAGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATgg                                                      >  1:286419/1‑143 (MQ=37)

        cTGTTTGAAGCCACCCACGGTATTTGCCCTAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTTAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTTATTGTTAAAGGTATGGa                                                     <  2:177607/143‑1 (MQ=25)

           tttGAAGCCACCCACGTTACTGCGCCGAAATATGCCGGTCAGGATAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGAAGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAgg                                                  <  1:675436/143‑1 (MQ=25)

                  ccacccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCCCTGAGATGATGCTGCGCCATATGGGTTGGACTGTATCGGCTGATCTGATTGTTAAAGGTATGAAAGCCGCAATc                                           >  1:113098/1‑143 (MQ=25)

                       cccGGTACTGCGCCGAAATTTGGCGGTTAGTAGAAAGTTAATTCGGGCTCTATTATTCTCTCCGCTGAGATGATGATGCGCCATATGGGTTGTACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc                                      <  2:539175/141‑1 (MQ=17)

                       cacGGTACTGCGCCATAATACGCCGGTCAGGACAAAGTAACTCCTGGCTCTATTATTCGCTCCGCTGATATGATGTTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc                                      <  2:403547/143‑1 (MQ=18)

                                 cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCTAAACGTTTCTAACTTAAGCGGCTGACCTGATTGTTAAAGGAATGGAAGGCGCAATCAATGCCAAGAGCGTa                            >  1:257901/1‑143 (MQ=18)

                                 cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCCTATGGGTTGGACTGAAGCGGCTGACATGAATGATAAAGATATAGAAGGCGCAATCAATGCCAAGACCGTa                            >  1:379915/1‑143 (MQ=21)

                                      aaaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAActta                       >  1:14311/1‑140 (MQ=17)

                                      aaaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATCCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGACGCGGATGACCTGATTGTTAAAAGTATGGAAGGCGCAATAAAAGCCTACACCGTCACCTa                       >  2:488971/1‑143 (MQ=18)

                                           tGCTGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGCTGCTGCGCCATATGGGTTGGCCTGAAGCGGCTGACCTGATCGTTAAAGGTATGGAAGGCGCAAACAATGCCAAGACCGTAACATATGAca                  >  1:531379/1‑142 (MQ=17)

                                           tGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCAGACATAACTGCCTAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACt                  >  2:353225/1‑143 (MQ=18)

                                             ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAAGCGCAATCAATGCCAAGACCGTAACTTATGACTTc                >  1:665817/1‑143 (MQ=17)

                                                     gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt        >  2:227623/1‑143 (MQ=14)

                                                           gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGACATATGGGTTGGAGTGAAGCAGATGACATGATTGATAAAGGTATGGAAGGAGAAATAAATGCAAAAACCGTAACTTATGACTTCGAACGTATgatgga  >  1:229466/1‑143 (MQ=14)

AATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGA  >  NC_000913/1196114‑1196315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`