Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC93.5% 81.0 / ‑4.8 31I204S (ATC→AGC) wcaAputative colanic acid biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base C (11/18);  total (13/18)
Fisher's exact test for biased strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132647‑2132925
                                                                                                                                            |                                                                                                                                          
gtaTCTGGTAGAGAGTAAACAGCTTATATTTTTTTCTGGCGCGGTTTAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTc                                                                                                                                          <  1:352738/141‑1 (MQ=255)
   tCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGGAGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTGTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                       <  1:81573/143‑1 (MQ=255)
   tCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGAGGTTGAATTTGTATTTGTGCATGCGGGAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                       <  1:117303/143‑1 (MQ=255)
        tAGAGGGTAAACAGCTGATATTTTTTGCTTTTGTTGTTGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                  <  1:666370/143‑1 (MQ=255)
         agagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGAGATCTGCAAATCACTGTGATTGATATGCAGGCTCTGCGTCGcc                                                                                                                                 >  1:783921/1‑143 (MQ=255)
            gggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctct                                                                                                                              <  1:659773/143‑1 (MQ=255)
                     gCTGATATTTTTTGTTGGCGCGGTCTATTTTGTCTTTGTTCTTGCTGTAAAAGTGGAAGTACCCGGAGAATGGTGACGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc                                                                                                                     <  1:89324/143‑1 (MQ=255)
                       tggttttttttGCTGGCGCTGTCTTATTTGTCTTTGTGCTTTCGGTAAAAGTGGAAGTACTCGGTGAATTTTTTCGGTGACGAGGTTATCTGCATTTCACCGTGATTGATATGCAGGCTCTGTGTCGCCTCTTCTACTTTCCa                                                                                                                   <  2:14770/138‑1 (MQ=255)
                          tatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACgg                                                                                                                <  1:393724/143‑1 (MQ=255)
                              ttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCAACGTGATTGATATGCAGGCTCTGCGTCACCTCTTCTACTTTCCACGGTTCg                                                                                                            >  2:106574/1‑143 (MQ=255)
                                    tGGCGCGGTCGAATTTGTGTTTGTACTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGAGCTGCGTCGCCTCTTGTACTTTCCACGGTGCGCAGtgc                                                                                                      >  2:285189/1‑141 (MQ=255)
                                           gTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGATGTACCCGGATAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacca                                                                                               <  1:203012/143‑1 (MQ=255)
                                            tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGTCGAGGTGATCTGCATTTCACAGTGATTGATATGAAGACTCTGCGTCGACTCTTCTACTTTCCACGGTTCGCCGGACTccaccac                                                                                              >  1:212918/1‑143 (MQ=255)
                                                aTTTGTCTTTGTGCTTCCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGCGATCTGCATATCACCGTGATTGACACGCACGCTCTGCGTCGCCTCTCCAACTTCCCCCGGTTCACCGTACTccaccaccacc                                                                                          >  2:329788/1‑141 (MQ=255)
                                                   tGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCTGGCTCTGCGTCGCTTCTTCTACTTTCCACGGTTCGCCGTACTTCACCACCATCCGc                                                                                       >  1:546020/1‑143 (MQ=255)
                                                         tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAg                                                                                 <  2:596930/143‑1 (MQ=255)
                                                               tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACAGTTCGCCGCACTCCACCACCATCCGCAAGaaaaagaag                                                                           >  2:470909/1‑136 (MQ=255)
                                                                gCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCCCAGGAAGCTGTCGt                                                                          >  2:224416/1‑143 (MQ=255)
                                                                  ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCAGCGTGATTAATATACAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTaa                                                                        <  1:600077/143‑1 (MQ=255)
                                                                          tGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACACCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGc                                                                >  1:140462/1‑143 (MQ=255)
                                                                            gAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATACAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCTTCCGCAGGAAGATGTCGTAATCCTGTGCCg                                                              <  2:516341/143‑1 (MQ=255)
                                                                                 aCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGGACCCCACCACCATCCGAAGGAAGATGTCGGAAGCCCGTGACGCGTTc                                                         >  2:784492/1‑143 (MQ=255)
                                                                                       agaATTTTTTCGGTGACGAGGTGATCTGCATTTCATCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                   <  1:429923/143‑1 (MQ=255)
                                                                                         aaattgttCTGTGCCGAGGTGATCTACATGTCGGCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCTCTGTTCGCGGTGCTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTGTCAGTTCGGt                                                 <  2:825097/137‑1 (MQ=255)
                                                                                             tgttCGGTGTGGGGGTGTTCTGCATGTCACCGTTATTGTTATGTGGGCTCTGCGTCGCCTCTTCTACTTGCCACGGTGCGCCGTACTCCTCGACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg                                             <  2:689845/141‑1 (MQ=255)
                                                                                               ttCGGTGCCGAGGTGGTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCATGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa                                           <  2:783921/143‑1 (MQ=255)
                                                                                                        gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTATAAGTACGATATCGAACAAACActc                                  >  1:430267/1‑143 (MQ=255)
                                                                                                                  gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTTCACGGTTTGTTGTATTCCATCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGc                        <  1:735107/143‑1 (MQ=255)
                                                                                                                           cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCCCCGGAAGATGTCGTAATCCTGTACCGCTATCAAATCGATAACGAACAAAAACACTTAGAAACGCAAtaacaat               >  1:45988/1‑137 (MQ=255)
                                                                                                                                ttgacATGCAGGCTCTGCTTTGCCTCTTCTACTTTCCACAGTTCGCCGTTCTCCACCACCATCCGTAGGAAGATCGCGAAAGCCTGGGCCGATTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa          <  1:108574/143‑1 (MQ=255)
                                                                                                                                        cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGCTGATGTCTTAATCCTCTACCGCATTCAAATCAGTATCGAACAAAAACTCTTTGAAACGCAATGCCCAGGTAAAAACCTGGt  >  1:749151/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                          
GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132647‑2132925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑